Objective:To explore the effects of 1,25-dihydroxyvitamin D3 on renal expression of TGF-?1 and HGF in type 1 diabetic rats.Methods:30 Sprague-Dawley rats were randomized into 3 groups:normal control rats(group NC),diabetic rats(group DM ) and diabetic rats treated with 1,25-dihydroxyvitamin D3(group DC).Kidney-to-body weight ratio(KW/BW),mean glomerular volume(MGV),fractional mesangial area(FMA),urinary albumin excretion rate(UAER)and serum creatinine(Scr)were measured after 12 weeks of treatment,while transforming growth factor-betal(TGF-?1) expression in kidney cortex were observed by immunohistochemistry.The expression levels of TGF-?1 mRNA and HGF mRNA were detected by reverse transcription and polymerase chain reaction(RT-PCR) at the end of the experiment.Results:UAER,Scr,MGV,FMA and KW/BW ratio were significantly higher in the diabetic rats than those in the normal control rats(P

Objective To study the clinical characteristics of abnormal lipid metabolism in different types of elderly people with hypertension.Methods The clinical data,collected from the elderly people who underwent annual physical examination,the characteristics of dyslipidemia and abnormal lipid metabolism in different types of elderly people with hypertension were analyzed retrospectively. Results The prevalence of dyslipidemia was 69. 63％, in which the prevalence of hypercholesteremia and hypertriglyceridemia was 23. 65％, and 26. 71％ respectively. The prevalence of hypercholesteremia accompanied hypertriglyceridemia( mixed group) was 19. 27％, and normal group was 30. 37％. Hypertriglyceridemia group was easier to be accompanied with hypertension than normal group and HTC group(P ＜0. 05). The hypertriglyceridemia had higher risk of cardiovascular and cerebrovascular diseases than norral group and HTC group( P ＜ 0. 05 ). Conclusion The elderly people had high prevalence of dyslipidemia and metabolic syndrome,and with high prevalence of hypertension, which led to higher risk of cardiovascular and cerebrovascular diseases. The hypertriglyceridemia had more strong impact on cardiovascular and cerebrovascular diseases than hypercholesteremia.

Long non-coding RNA (lncRNA) is a group of functional RNA molecules, which is more than 200 nu-cleotides in length, lacks ability of encoding protein and participates in all aspects of gene regulation. The current studies have indicated that some lncRNAs take part in regulating the process of breast cancer tumorigenesis and progression. This article reviewed the abnormal expression of lncRNA in breast cancer, its relationship to breast cancer growth, apoptosis, invasion and metastasis, and its function in early diagnosis, drug resistance and prognosis of breast cancer. This review was expected to offer an insight into a new target for the prediction, diagnosis and individual treatment of breast cancer.

Objective Compared with surgical operation,to investigate the clinical efficacy and advantages of interventional treatment in blunt liver traum.Methods 32 patients from July 2010 to October 2012,who were diagnosed as blunt liver trauma,received super selective hepatic artery embolization in Chinese People's Liberation Army in 98th Hospital.Another 27 ones received operation at the same time in Peolies's Liberation Army 98th (AAST grade Ⅱ to Ⅳ).Retrospective analysis of related cases was presented.The results were analyzed by t test.Results All of 32 patients that received super selective hepatic artery embolization were stanched bleeding successfully.All patients were followed up for 3 to 18 months,the mean follow-up time was 10 months,with no severe complications occurred or no lost cases.Conclusions Compared with surgical operation,interventional treatment had advantage that included eliable hemostasis,less operation time,minimally invasive and less hospitalization time.Interventional treatment had good effects on blunt liver trauma which were AAST grade Ⅱ to Ⅲ.

AIM: To study the effect of herbal compound poria prescription (CPP) on proliferation and differentiation in primary rat preadipocytes, and to explore the mechanism of this process. METHODS: Primary cultured rat preadipocyte proliferation was determined by cell counting and MTT spectrophotometry. Cell differentiation was determined by Oil Red O staining, and the expression of PPAR? mRNA was determined by RT-PCR. RESULTS: CPP inhibited the proliferation and differentiation of primary rat preadipocytes in a dose-dependent manner, and it also inhibited the expression of PPAR? mRNA. CONCLUSION: CPP inhibits the proliferation of primary rat preadipocytes and inhibits the preadipocyte differentiation by suppressing the expression of PPAR? mRNA.

Objective To construct the eukaryotic expression vector of Notch1intracellular domain,p3XFLAG?CMV7?NICD1,so as to prepare for the further research and exploration of effect of Notch1 on promoting epithelial?mesenchymal transition of human lens epithelial cells. Methods The cDNA fragment was reversely transcribed by RT?PCR from total RNA extracted from the SRA01/04 cells and was encoded with the specific am?plification?targeted NICD1was obtained from the SRA01/04 cells,then the cDNA fragment was inserted into p3XFLAG?CMV7 to transcribe Esche?richia coli DH5α. And the recombinant plasmid was extracted after bacterial screening by LB plating medium and confirmed by the restriction endo?nuclease digestion and DNA sequencing. Results The target gene obtained had the same molecular size as predicted. It was indicated that recom?bined p3XFLAG?CMV7 plasmid contained correct recombinant human Notch1 sequences and p3XFLAG?CMV7?NICD1 was constructed success?fully. The western blotting showed protein NICD1 expressed in SRA01/04 cells transfected with p3XFLAG?CMV7?NICD1. Conclusion The suc?cessful construction of p3XFLAG?CMV7?NICD1 will provide a foundation for a further study studies in on the effect relationship of Notch signaling pathway and in posterior capsular opacification(PCO)after cataract extraction.

BACKGROUND:Bronchial asthma is considered general y to have an association with Th2 immune response disease, but there is no ideal treatment. Bone marrow mesenchymal stem cel s as a kind of adult stem cel s not only have the multipotent differentiation and proliferation capacity, but also have low immunogenicity and immunoregulation ability. OBJECTIVE:To investigate the effect of culture supernatant of bone marrow mesenchymal stem cel s on lung inflammation of bronchial asthma mice. METHODS:Twenty experimental mice were randomly divided into control and experimental groups, 10 mice in each group. At 0 and 14 days, intraperitoneal injection of ovalbumin induced sensitization in mice, and at 24-26 days, aerosolized ovalbumin solution was used for excitation. From the 24th day, in the experimental group, bone marrow mesenchymal stem cel supernatant was intraperitoneal y injected at 2 hours before excitation;meanwhile, normal saline was injected in the control group. At the last of excitation, the mice were sacrificed under anesthesia to take serum samples, bronchoalveolar lavage fluid and lung tissues. RESULTS AND CONCLUSION:(1) Mice in the control group appeared to have abnormal lung tissue structure, and there were a large amount of eosinophils and monocytes in the submucosa and muscularis. However, lung inflammation was relieved in the experimental group after bone marrow mesenchymal stem cel treatment. (2) The levels of interleukin-17 in the bronchoalveolar lavage fluid and serum were significantly lower in the experimental group than the control group (P<0.05), but there was no difference in the levels of interleukin-4 between the two groups (P>0.05). These findings indicate that the intraperitoneal injection of bone marrow mesenchymal stem cel s can ease lung inflammation and reduce levels of inflammatory markers in the bronchoalveolar lavage fluid and serum of bronchial asthma mice.

Objective To investigate the effect of electroacupuncture on cervical surface electromyographic signals and explore the mechanism of its action on cervical muscle fatigue in patients with cervical spondylosis.Method All enrolled patients with cervical spondylosis received electroacupuncture treatment. Surface electromyography was performed in the patients before and after the first and one course of electroacupuncture treatment. An evaluation was made using a short-form McGill Pain Questionnaire before and after one course of electroacupuncture treatment. Pre- and post-treatment surface electromyographic RMS values and questionnaire scores were statistically analyzed and compared within the group.Result There were statistically significant differences in cervical erector spinae and upper trapezius RMS values during head lateral flexion and backward extension between before and after the first or one course of treatment (P<0.05). There were statistically significant differences in the questionnaire sub-scores between before and after one course of treatment (P<0.05).Conclusion Electroacupuncture can markedly reduce cervical muscle fatigue in patients with cervical spondylosis. Surface electromyography as a noninvasive and objective evaluation method has a broad application prospect.

Objective To understand the issues in the conversion of private medical institutions and explore the possible options.Methods Case study and in-depth interview were employed in 6 converted medical institutions.Results The reasons for conversion were diversified,and the process was not standardized.The attitudes towards how to deal with the net assets differed so much among the executives.The legislation and supervision by the government were still under-developed.Conclusion The process for the conversion should be standardized and the ownership of the net assets should be clearly defined.The legislation and supervision on private medical institutions should be enhanced.

Objective: T o further investigate pathogenesis about vulvovaginal candi diasis (VVC). Methods: Human normal vaginal epithelial cells in vitro were cultured by tissue cultur e and keratinocyto serum-free medium. Passaging epithelial cells were coculture d with candida albicans medium in separate wells for 0, 3, 6, 12, 24, and 48 h. C ontr ol epithelial cells were cultured alone in separate wells. For examination o f cytokines and chemokines, at each time point, the coculture supernatant and th e control culture were collected. ELISA was applied to detect the levels of HBD -1, HBD-2 and SP-A protein expression in the vaginal epithelial cells (HBD-1 :F= 62.784,P=0.001; HBD-2: F=5127.984, P=0.000; F=542.210, P=0 .000). Also, we colle cted the vaginal doughing water of VVC patients and healthy women, and detected the levels of SP-A, then analysed the data. Results: In the tra il of human vagin al epithelial cells, in contrast to the control group, the medium group induced the epithelial cells to secrete more HBD-1, HBD-2 and SP-A at h48, which was co nsidered to be significant (P