Certification ; Laboratories

BACKGROUND: In hospital laboratory using Microscan, the search for an isolate or the analysis for antimicrobial susceptibility rates were obtained by the Data Management System (DMS) software. However, it is hard to convert DMS database to other file formats in addition to some limitation in using the database. We applied BacLink 2 and WHONET 5.1 softwares to convert and analyse DMS database for the utilization of the isolate profiles and the antimicrobial resistance rates. METHODS: Specimen and microbial data were printed as 'Short report form', an ASCII text file, from Microscan DMS. BacLink 2 software was used to convert the printed file to dBASE format file. Statistical analyses were performed using WHONET 5.1 software. RESULTS: Data of isolates were obtained as 'Short report form' in one month intervals. This file could be converted to other database file using BacLink 2 software. The antimicrobial resistant profiles were obtained, and the susceptibility, intermediate resistant, and resistant rates for each isolates could be analyzed. CONCLUSIONS: In this study, BacLink 2 and WHONET 5.1 software were successfully applied for the conversion of the database. Analysis of isolate profiles and antimicrobial resistant rates could be performed in other personal computer systems. The database management by BacLink 2 and WHONET 5.1 software could be applicable for the convenient statistical analysis in microbiology laboratories using Microscan.
Laboratories, Hospital ; Microcomputers

In West Germany the Giessen-test (G.T) is one widespread personality test, specially in the stress – related disorders. Giessen-test itself consists of 40 items which is grouped into 6 main scales. 183 healthy students are studied by this test. In comparison the result of G.T in medical students of Ha Noi and in Bulgarian young people, there are significant differences in 4 scales of this test (scale 2; 3; 5; 6). However, the difference in 2 scales (scale 1; scale 4) is uncertain. The difference between male and female students is found insignificantly in our research according to G.T scales. In order to have the result of exact standardization, we must research this test on the population larger with different ages and professions.
Students, Medical ; standards ; laboratories

In 2002, DHF viro-serological surveillance was started in 19 Southern provinces of Vietnam. 247 DEN virus strains (consist of 27 DEN-1, 99 DEN-2, 9 DEN-3 and 112 DEN-4) were isolated from 3318 blood samples. The predominant serotypes were DEN-4 (45.3%) and DEN-2 (40.1%). DEN-2 virus was more positively correlated with severe cases in comparison with other serotypes. However, high rates of virus isolation were obtained from DHF grade I-II patients (49%), and then from DF, viral infection and fever of unknown origin cases (40.1%). These should be main target subjects for the active surveillance of DHF. In addition, MAC-ELISA was done on 7498 DHF serum samples taken in Southern Vietnam with the average positive rate of 44.37%
Dengue ; epidemiology ; Laboratories

This report described a program undertaken with the collaboration of the CDC (Colorado, USA) known as "Active, laboratory-based surveillance for dengue infection in Bien Hoa city in 2002". Results of this study are as follows: The predominant serotype which circulated in Bien Hoa city in 2002 was Dengue-4. The number of children Dengue ; epidemiology ; laboratories

One method to determine the content of chitosan present in Pokysan cream has been developed. Chitosan was separated from Pokysan by dissolving the cream in acidic solution, extracted by chemical mixtures of I and II, which were prepared by laboratory of polymers in medico – pharmacy, and then collecting the precipitate of chitosan in the alkaline medium. This precipitate was then qualified by Lugol reagent and IR spectra. Collected chitosan precipitate was also qualified and quantified. These results proved that chitosan has been included in Pokysan cream and our method is confident
Chitosan ; Evaluation Studies ; Laboratories

Experiments were caried out on An.minimus F98 in laboratory conditions of 22+/- 1°C, 25+/- 1°C and 28 +/- 1°C, 70-80% RH. The time required for egg stage and egg hatching rate were negatively correlated with temperature. At 22 +/- 1°C, egg hatching rate was 84.1 %, the duration of the egg stage was 3-4 days. At 25+/-1°C and 28 +/-1oC, egg hatching rate was 77.3% and 65.5%, respectively, the time of the egg stage were 2-3 days. At higher temperature, the duration of larvae stage was shorter than at the lower temperature. 25°C was optimum temperature for larvae with 38% of larvae developed to adults. 1 day after molting, mosquitoes started feeding at all tested temperatures. At 22°C, blood feeding rate higher than at the higher temperatures. Number of eggs and egg hatching rate of mosquitoes that fed human blood were higher than mosquitoes fed on mice
Anopheles ; Temperature ; Blood ; Laboratories

The evaluation trial was conducted from August 2004 to March 2005 in the laboratory of the National Institute of Malariolgy, Parasitology and Entomology, Ha Noi. Bioassays with An.dirus and Ae.aegypti (reared mosquitoes) were made according to the WHO method for evaluation of susceptibility and residual effects of the insecticide on the painted and nylon wall surface. Ale lose sprayed at the dose of 30mg ai/m2 were found to produce residual effect for 6-7 months on the painted wall surfaces (mosquito mortalities: 51.1%-67.8%) and for 4-5 months on nylon wall surface (mosquito mortality: 55.6%-67.8%).
Malaria ; Pyrethrins ; Laboratories

Through pH determination, acide- color quantification, extraction time and stabilization time of solvent extracting layer, optimal parameters were determined to quantify loperamide. The method could have high sensitive and selectivity with precise and repetitive results; its is appropriate for laboratories at low level of technique equipments
Loperamide ; Pharmaceutical Preparations ; Laboratories

The eggs of Anopheles dirus originated from China and currently colonized at the National Institute of Malariology, Parasitology and Entomology kept at 24 - 26°C on wet cotton for 20 - 25 days gave hatching rate approximately of 40%. The suitable food for Anopheles dirus larvae consisted of 6g bread powder, 2g green bean powder and 0.5mg vitamin B 1. The appropriate density for rearing An. dirus larvae is 0.4 larva per square centimetre of water surface
Malaria ; Anopheles ; Laboratories