Certification ; Laboratories

BACKGROUND: In hospital laboratory using Microscan, the search for an isolate or the analysis for antimicrobial susceptibility rates were obtained by the Data Management System (DMS) software. However, it is hard to convert DMS database to other file formats in addition to some limitation in using the database. We applied BacLink 2 and WHONET 5.1 softwares to convert and analyse DMS database for the utilization of the isolate profiles and the antimicrobial resistance rates. METHODS: Specimen and microbial data were printed as 'Short report form', an ASCII text file, from Microscan DMS. BacLink 2 software was used to convert the printed file to dBASE format file. Statistical analyses were performed using WHONET 5.1 software. RESULTS: Data of isolates were obtained as 'Short report form' in one month intervals. This file could be converted to other database file using BacLink 2 software. The antimicrobial resistant profiles were obtained, and the susceptibility, intermediate resistant, and resistant rates for each isolates could be analyzed. CONCLUSIONS: In this study, BacLink 2 and WHONET 5.1 software were successfully applied for the conversion of the database. Analysis of isolate profiles and antimicrobial resistant rates could be performed in other personal computer systems. The database management by BacLink 2 and WHONET 5.1 software could be applicable for the convenient statistical analysis in microbiology laboratories using Microscan.
Laboratories, Hospital ; Microcomputers

In West Germany the Giessen-test (G.T) is one widespread personality test, specially in the stress – related disorders. Giessen-test itself consists of 40 items which is grouped into 6 main scales. 183 healthy students are studied by this test. In comparison the result of G.T in medical students of Ha Noi and in Bulgarian young people, there are significant differences in 4 scales of this test (scale 2; 3; 5; 6). However, the difference in 2 scales (scale 1; scale 4) is uncertain. The difference between male and female students is found insignificantly in our research according to G.T scales. In order to have the result of exact standardization, we must research this test on the population larger with different ages and professions.
Students, Medical ; standards ; laboratories

In 2002, DHF viro-serological surveillance was started in 19 Southern provinces of Vietnam. 247 DEN virus strains (consist of 27 DEN-1, 99 DEN-2, 9 DEN-3 and 112 DEN-4) were isolated from 3318 blood samples. The predominant serotypes were DEN-4 (45.3%) and DEN-2 (40.1%). DEN-2 virus was more positively correlated with severe cases in comparison with other serotypes. However, high rates of virus isolation were obtained from DHF grade I-II patients (49%), and then from DF, viral infection and fever of unknown origin cases (40.1%). These should be main target subjects for the active surveillance of DHF. In addition, MAC-ELISA was done on 7498 DHF serum samples taken in Southern Vietnam with the average positive rate of 44.37%
Dengue ; epidemiology ; Laboratories

This report described a program undertaken with the collaboration of the CDC (Colorado, USA) known as "Active, laboratory-based surveillance for dengue infection in Bien Hoa city in 2002". Results of this study are as follows: The predominant serotype which circulated in Bien Hoa city in 2002 was Dengue-4. The number of children Dengue ; epidemiology ; laboratories

One method to determine the content of chitosan present in Pokysan cream has been developed. Chitosan was separated from Pokysan by dissolving the cream in acidic solution, extracted by chemical mixtures of I and II, which were prepared by laboratory of polymers in medico – pharmacy, and then collecting the precipitate of chitosan in the alkaline medium. This precipitate was then qualified by Lugol reagent and IR spectra. Collected chitosan precipitate was also qualified and quantified. These results proved that chitosan has been included in Pokysan cream and our method is confident
Chitosan ; Evaluation Studies ; Laboratories

The Biomedical Laboratory Center of Hanoi Medical University was established in January 17th 1997 and comprises 4 small labors: the functional tests; biochemical; immunology and genetic. According to its functions and tasks, the labor has human resources with the high technical and scientific levels. This resource originated from the faculties of Hanoi Medical University. This is an activity pattern which is suitable and convenient for staffs and students in the university.
Technology Assessment, Biomedical ; Laboratories

The survey covered 11 cities/provinces with 179 servey copies distributed to staffs involved in microbiological testing during 2003-2004. Results showed that tested microbiological samples of water and food were 790±817 and 699± 552 (x±SD)/year, respectively, with 4-5 testing indicators applying for MPN7-9 tubes. The number of samples for testing microorganism in the environment was still limited. In five years of 2000-2004, epidemics occurred in some provinces like typhoid, cholera and dengue fever. Those were tested for causes making up 88% and the rate of success was high. Some causes for epidemics (typhoid, cholera, dengue fever and pacillary dysentery) can be identified at provincial centres of preventive health.
Preventive Medicine ; Laboratories

Clinical and laboratory findings was investigated on 46 patients who had diagnosis of lupus glomerulonephritis type IV. The obtained results showed that clinical features are severe with 45.7% patients had hypertension, 34.8% patients had acute nephritis syndrome and 56.5% had nephrotic syndrome. Especially, there were 95.7% patients have renal insufficiency. Among them, 26.1% had a severe renal failure (blood creatinine level >300mmol/I). But after 1 month of treatment, 100% of renal insufficiency patients have blood creatinine level < 300mmol/l and 72.8% of renal failure patients who had creatinine level > 300mmol/l had improvement in renal function
Lupus Nephritis, Laboratories, Diagnosis

Vnukovo-32 a cell culture rabies is used to study the vaccine strain technology of inactivated culture rabies vaccine production in primary hamster kidney cells. The master and working seeds meet the criteria of rabies vaccine strain titer (5.5 log LD 50 - 7.1 log LD 50/ml) according to WHO standard. During the study, we have used the procedure for inactivated, non concentrated rabies vaccine production of Russia and other countries procedures as references. The result was we constructed the procedure for inactivated, concentrated cell culture rabies vaccine production at laboratory scale.
Rabies Vaccines ; Cells, Laboratories