Objective To study the etiology of surgical infection and antibiotic resistance of infective bacteria,so as to provide evidence for clinical treatment.Method The composition and antibiotic resistance in surgical specimens of pathogenic bacteria from January 2008 to December 2010 was analyzed retrospectively.Results A total of 321 pathogens isolated,Gram-negative bacteria 201,accounting for 62.6％,Gram-positive cocci 114,accounting for 35.5％,Fungus 6,accounting for 1.9％.Escherichia coli 116(36.1％),Klebsiella pneumoniae 24(7.5％),Staphylococcus aureus 34(10.6％),Pseudomonas aeruginosa 18(5.6％),Enterococcus faecalis 16(5.0％).Staphylococcus species of Staphylococci methicillinresistant Staphylococcus atureus(MRS),Coagulase-negative staphylococci MRS and ultra wide-spectrum beta-lactamases(ESBLs)detection rate was 52.9％(18/34),83.7％(36/43)and 35.0％(49/140),respectively.The resistance rates of Gram-negative bacilli to piperacillin/tazobactam,ceftazidime,amikacin,imipenem and meropenem were below 20％,Gram-positive cocci,staphylococci to new compound sulfamethoxazole,minocycline,rifampin,vancomycin,teicoplanin and fusidic acid resistance rates were less than 40％,various pathogens had varies resistance.Conclusions Specimens of surgical infection pathogens widely distribute in different drug-resistant bacteria,some strains of multi-drug resistant infection,causing clinical failure of empirical therapy,clinical laboratory susceptibility results should be based on the rational use of antibiotics,to avoid the overuse of antibiotics.

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

To establish a new method for the determination of chondroitin sulfate. The method was based on the interaction of sodium chondroitin sulfate with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to yield a violet product, 6-mercapto-3-substituted-s-triazolo-\[4,3-b\]-s-tetrzaine derivatives. Absorbance at 540 nm obeyed Beer's Law within the concentration range from 0.1 to 1.0 g?L~(-1)and the results were precise and accurate. The method was operationally simple and had a good precision and sensitivity.

Objective To study the influences of chitosan on swimming time, changes of blood glucose and lactic acid in mice. Methods The test groups were given chitosan by oral administration,and eighteen days later, the swimming time,blood glucose and lactic acid were determined. Results The swimming time of test groups was obviously longer than that of the control group.After thirty-minutes swimming, the elimination of blood lactic acid and the recover of blood glucose were distinctly faster than those of the control group. Conclusion Chitosan had anti-fatigue effect on mice.

Objective To explore the efficacy and protective mechanism of calpain, a calcium activated neutral proteinase as a vaccine candidate molecule. Methods Anti calpain sera were prepared by immunized BALB/c mice with recombinant calpain. Anti calpain sera, schistosomula of Schistosoma japonicum , and mouse eosinophils were incubated at 37 ?C , 5% CO 2 for 48 hours, and the adherence of eosinophils to schistosomula and its schistosomulacidal efficacy were observed. Results Mice immunized with recombinant calpain produced a high level IgG antibodies specific to the antigen immunized. Immunoblotting analysis showed murine anti recombinant calpain sera bound specifically to recombinant calpain. Ninety six percent of schistosomula were surrounded by cells when incubated with mouse eosinophils, and a significant number of dead schistosomula was observed at 48 hours when incubated with mouse serum and eosinophils as compared with control serum and eosinophils ( P

Objective We discussed the optimal method for intestinal preparation before colonoscopy examination in order to increase the success rate of intestinal examination and reduce the incidence of adverse effect. Methods We divided 120 patients who were to undergo colonoscopy examination into group A, B and C with 40 cases in each group. Group A received oral magensium sulfate 100ml and 6000ml water after that on the morning of the examination. Group B received oral magensium sulfate 50ml and 2000ml in the evening before the examination, oral magensium sulfate 50ml and 400ml water on the morning of the examination. Group C was given oral magensium sulfate 100ml and 4000～5000 water at the same time. Group A and B took part in appropriate activity and were given health education. The effect of intestinal preparation was compared between the three groups. Results The cleaning degree of group B was better than those of the other two groups (P<0.05). The adverse effect of group B was less than that of group C (P<0.01). Conclusions Oral intake of magensium sulfate in the evening before and on the morning of the examination for intestinal preparation could increase the cleaning degree of intestine, facilitate the observation of disease part and lessen adverse effect. It gave satisfying examination results and made patients satisfied.

Objective To evaluate the laparoscopic hysterectomy surgery continuous infusion of low-dose sufentanil combined with propofol and remifentanil anesthesia postoperative hyperalgesia influence.Methods Patients were collected from June 2013 to June 2015 in our hospital undergoing laparoscopic hysterectomy 60 cases.According to the principles of randomized, divided into sufentanil group (SF group) and 30 cases of normal saline group (NS group) 30 cases.Two intraoperative remifentanil 0.1-0.2 μg/(kg? min) and propofol 4-8 mg/(kg? h) to maintain anesthesia.After the operation began, SF group and NS group were sufentanil and saline.Record calls eyes, recovery of spontaneous breathing, extubation time;after waking 5 min, 30 min, 12 h, 24 h, 48 h pain (visual analogue scales, VAS) score; before induction (T0), after induction (T1), after intubation (T2), surgery (T3), extubation (T4), after extubation 10 min (T5) heart rate (HR) and mean arterial pressure (MAP); excessive sedation, respiratory depression, intraoperative awareness, restless when awake, intraoperative after nausea/vomiting, tramadol and so on.Results SF group and NS group recovery of spontaneous breathing, eye opening and extubation call time was not statistically significant;after SF group wake 5 min, 30 min and 4 h VAS scores lower than the NS group (P <0.05); in T4, T5 point comparison, SF group MAP and HR lower than the NS group (P <0.05); SF group restless when awake, pain (VAS score≥3), postoperative use of tramadol was lower than NS group (P<0.05).Conclusion Intraoperative continuous infusion of low-dose sufentanil improve laparoscopic hysterectomy in patients under anesthesia using propofol combined with remifentanil hyperalgesia, with good prospects for clinical application of anesthesia.

Objective To investigate the efficacy of intensity-modulated radiation therapy(IMRT)and the prognosis factors in advanced cervical cancer.Methods A total of 218 cases of cervical cancer patients treated in Affiliated Tumor Hospital,Guangxi Medical University,between July 2007 and July 2012,were divided into IMRT group and routine radiotherapy group (conventional group,94 vs 124 cases).To compare the short-term effects of two groups by Chi-square test,and the incidence rate of 1,3,5-year survival by rank sum test ; to compare the acute and chronic toxicity of the two groups by rank sum test.And to analysis prognostic factors by log-rank test and Cox proportional hazard model and multiple linear regression analysis.Results (1) The effective rate in IMRT group was 95.7％ (90/94),which in the conventional group was 86.3％ (107/124; P ＜0.01).The 1,3,5-year overall survival rates in IMRT group were not significant difference than those in conventional group (87.6％ vs 90.1％,73.4％ vs 81.5％,and 72.3 ％ vs 75.7％ ;all P ＞ 0.05).The rate of acute gastrointestinal toxicity was 41.5 ％ (39/94) in IMRT group,vs 42.7％ (53/124) in conventional group(P ＜ 0.01); and the incidence of acute hematopoietic toxicity in IMRT group was 76.6％ (72/94),which was 78.2％ (97/124) in conventional group (P ＜0.01) ; the incidence of acute genitourinary toxicity was 12.8％ (12/94) in IMRT and 8.1％ (10/124) in conventional group(P =0.248).The late gastrointestinal,genitourinary and hematopoietic toxicities were 18.1％ (17/94),16.0％ (15/94) and 25.5％ (24/94),which were lower than those in conventional group 91.9％ (114/124),47.6％ (59/124) and 56.4％ (70/124),respectively (all P ＜0.01).(2) Univariate analysis showed that the International Federation of Gynecology and Obstetrics (FIGO) stage,histological grade and tumor size were closely associated with 5 years disease-free survival rates and overall survival rates (all P ＜ 0.05).Multivariate analysis showed that FIGO stage and histological grade were closely associated with 5 year overall survival rates (all P ＜ 0.01).FIGO stage and histological grade were independent factors to patients' 5-year overall survival rates in cervical cancer (P ＜0.01).Conclusion IMRT would be to reduce the acute and chronic toxicity,and to improve the quality of patients' life,but did not improve the 5 years survival rate in advanced cervical carcinoma.

Objective To observe the effect of Dahuangzhechongwan on protein excretion, serum cholesterol, TXB_2 and 6-keto-PGF_(1?) in rats with adriamycin-induced nephrosclerosis. Methods The rats received twice-intravenous injections of adriamycin after one kidney was removed, and were fed with hyperlipide food to establish an animal model for diffusion mesangioproliferation following with focal segmental glomerulosclerosis. The rats were randomly divided into shame-operated group, adriamycin nephrosclerosis group, Dahuangzhechongwan group, benazepril group. Urinary protein excretion, blood fat, TXB_2 and 6-keto-PGF_(1?) were observed. Result Proteinuria extract was decreased, the plasma cholesterol was significantly lower, the level of TXB_2 was cut down and 6-Ket-PGF_(1?) was increased by Dahuangzhechongwan. Conclusion Dahuangzhechongwan has the role of simutianeous application of elimination and tonification, promoting the circulation of Qi and blood, eliminating blood stasis, improving the hemodynamics. Dahuangzhechongwan has the renal protection on rat with adriamycin-induced nephrosclerosis, the mechanism may be relate with adjusting the balance of TXB_2 and 6-keto-PGF_(1?).