Objective To investigate the implications of gene expression pattern of pancreatic endocrine differentiation from mouse embryonic stem cells (mES) in vitro.Methods Mouse ES cell line ES W9.5 was cultured and differentiated into pancreatic endoerine progenitor cells by using the stepwise differentiation strategy in vitro,then stage specific genes expression were tested by RT-PCR and immnunoeytochemistry.The Illumina Mouse Ref-8 vl.1 Expression BeadChips were used to compare the changes in the transcriptome of mES-derived differentiated cells on day4,8,15,20,22,25 after differentiation to undifferentiated mES cells.Results A total 86 differentially expressed genes,both up- and down-regulated,were found during the whole differentiation stage,6 gene clusters with the same developing patterns and differentially expressed genes in 6 groups were collected.By number,the most differentially expressed genes corresponded to posterior foregut stage( 201 genes) followed by definitive endoderm stage ( 17 genes).Conclusions The analysis of the genes with the same developing pattern and differentially expressed genes at each differentiation stage during pancreas development by microarray analysis provides some evidences of early embryonic and pancreatic development.

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Cardiac Surgical Procedures ; adverse effects ; Coma ; etiology ; psychology ; therapy ; Consciousness ; Female ; Heart Diseases ; surgery ; Humans ; Male ; Middle Aged ; Young Adult

Ascomycota ; isolation & purification ; pathogenicity ; Female ; Humans ; Respiratory Hypersensitivity ; microbiology ; Sinusitis ; microbiology ; Young Adult

Objective To analyze the influencing factors of hemoglobin A1c in type 2 diabetic patients,including blood glucose,anti-diabetic therapeutic regiments,disease course,age,diabetic education,degree-educated,self-monitoring blood glucose.Methods Seventy-eight type 2 diabetic patients were divided into two groups by different degree of blood glucose,anti-diabetic therapeutic regiments,disease course,age,diabetic education,degree-educated,self-monitoring blood glucose.The levels of hemoglobin A1c in two groups were compared.Results There were significant changes in the levels of hemoglobin A1c between fasting blood glucose (FBG) ≤ 6.1 mmol/L group (30 cases) and FBG ＞ 6.1 mmol/L group (48cases),2 h postprandial blood glucose (2hBG) ≤8.0 mmol/L group (32 cases) and 2hBG ＞ 8.0 mmol/L group (46 cases ),insulin treatment group (27 cases) and no-insulin treatment group ( 51 cases ),combined drugs treatment group ( 36 cases ) and single drug treatment group ( 15 cases),age ≤ 65 years old group (41cases) and age ＞65 years old group (37 cases),disease course≤ 10 years group (39 cases) and disease course ＞ 10 years group( 39 cases),degree-educated above high grade group( 34 cases) and degree-educated below high grade group (44 cases),diabetic education ≥2 times/month group (20 cases) and diabetic education ＜ 2 times/month group (58 cases),self-monitoring blood glucose≥2 times/week group ( 19 cases)and self-monitoring blood glucose ＜ 2 times/week group (59 cases)[ (6.7 ± 1.5 )％ vs.(7.9 ± 1.3 )％,(6.8 ±1.1)％ vs.(7.8 ± 1.2)％,(6.7 ± 1.5)％ vs.(8.1 ± 1.4)％,(6.8 ± l.0)％vs.(8.0 ± 1.6)％,(6.9 ± 1.7)％ vs.(7.4 ± 1.6)％,(6.5 ± 1.2)％ vs.(8.2 ± 1.3)％,(6.9 ± 1.0)％ vs.(7.6 ± 1.4)％,(6.1 ± 1.7)％ vs.(8.0 ±1.1 ) ％,(6.7 ± 1.1 )％ vs.(7.6 ± 1.2)％ ] (P ＜ 0.01 or ＜ 0.05 ).Conclusion Blood glucose,anti-diabetic therapeutic regiments,disease course,diabetic,degree-educated,self-monitoring blood glucose are the important factors influencing hemoglobin A1c.

Objective To investigate the endovascular treatment of peripheral vascular anomaly in children and its efficacy.Methods Eighty-three children with peripheral vascular anomaly were treated via endovascular means. Before and during the endovascular operation,all the cases were diagnosed as follows: arteriovenous fistula in 23 cases, cirsoid angioma 32 and cavernous hemangioma 28. The endovascular approaches included:①transcatheter embolization using coils or gelfoam sponge;② transcatheter embolization combined with local cirrhosis agents treatment.Results Immediate angiography after embolization showed all the arteriovenous fistulas and hemangiomas were efficiently occluded and the clinical presentation and symptoms improved greatly. Follow-up one to three years after operation demonstrated 100% of efficacy and 68.7% curative rate with no complications. Conclusions Endovascular approach for peripheral vascular anomaly in children is a micro-invasive, safe and effective way.

Objective:Study the relationship of HLA I and B7 1 to elucidate the role of costimulatory molecule on antigen presentation in immunological rejection.Methods:FCM was applied to detect the expression of B7 1 Then the cytotoxicity of PBML was observed before and after blocking the HLA I Results:The level of HLA I expression increased greatly(P

Clinical features of 2 cases of ectopic ACTH syndrome with manifestation of severe hypercortisonism are described with the aim of exploring effective therapies.

Objective To explore the values of apparent diffusion coefficients (ADC) in staging the lesions of multiple sclerosis (MS) through analysing the differences of ADC in different types of lesions.Methods 137 lesions identified in 29 patients with MS,were characterized by their enhancement pattern on contrast-enhanced T1-weighted MR images and whether they were changing in appearance, size or signal.The lesions were classified into four groups:homogeneously enhancing lesions (HELs), ring-enhancing lesions (RELs), active nonenhancing lesions (ANELs), and inactive nonenhancing lesions (INELs).The mean ADC values for each type of lesions were calculated and compared.Results The ADC values for HELs, RELs, ANELs and INELs were (91.09±13.63)× 10 5 · m2 · s-1, (105.66±9.92) × 10-5 · m2 · s-1, (136.80±20.31) × 10-5 · m2 · s-1 and (127.46±13.65) ×10-5 · m2 · s-1.There were significant differences between the ADC values of any two groups in the four groups except the ADC values between ANELs and INELs.Conclusion ADC values are useful to differentiate each type of lesion,which can help stage the lesions of MS.

OBJECTIVE: To evaluate the efficacy and toxicity of sorafenib plus Capecitabine in patients with advanced hepatocellular carcinoma (HCC). METHODS: 20 patients (treatment group) were assigned to take sorafenib 200 mg bid for 3 consecutive weeks plus capecitabine 1 500 mg? m-2?d-1 for l4 days followed by 7 days discontinuation in 3-week treatment cycle. 22 patients in the control group only received Capecitabine 1 500 mg?m-2?d-1 for l4 days followed by 7 days discontinuation in a 3-week treatment cycle. Tumor response was assessed after 2-cycle treatment using modified WHO criteria. RESULTS: In the treatment group and the control group: the median survival times were 10.9 months and 7.2 months, respectively; the median time for tumor progression was 6.8 months and 4.3 months, respectively; the overall response rates were 20.0% and 9.1% respectively; the clinical benefit rates were 70.0% and 40.9%; the ?-foetoprotein (AFP) reduction rates were 65.5% and 39.0%, respectively. The toxicities were not significant between the two groups. CONCLUSION: Sorafenib plus Capecitabine is safe and effective for advanced hepatocellular carcinoma patients.

OBJECTIVE To evaluate the curative effects of surgery combined with early radiotherapy and local injection of hormonal anti-scarring drugs in the treatment of keloid in head and neck region. METHODS 35 patients with keloid were treated with surgery(completely excised and sutured directly or treated with local skin flap).Radiotherapy was applied within 24 hours after operation.A total dose of 20 Gy was administered in ten fractions of 2 Gy every day. Patients who had recurrences were treated with local injection of hormonal anti-scarring drugs.RESULTS After operation,all the wounds were healed by first intention.After 1-4 year follow-up,25 cases(71.43%) were cured without relapse;10 cases(28.57%) showed partial remission.The rate of therapeutic effect was 100%.CONCLUSION Surgical removal combined with local radiation and local injection of hormonal anti-scarring drugs are one of the effective methods in treatment with keloid in head and neck region.