Ascomycota ; isolation & purification ; pathogenicity ; Female ; Humans ; Respiratory Hypersensitivity ; microbiology ; Sinusitis ; microbiology ; Young Adult

Objective To investigate the implications of gene expression pattern of pancreatic endocrine differentiation from mouse embryonic stem cells (mES) in vitro.Methods Mouse ES cell line ES W9.5 was cultured and differentiated into pancreatic endoerine progenitor cells by using the stepwise differentiation strategy in vitro,then stage specific genes expression were tested by RT-PCR and immnunoeytochemistry.The Illumina Mouse Ref-8 vl.1 Expression BeadChips were used to compare the changes in the transcriptome of mES-derived differentiated cells on day4,8,15,20,22,25 after differentiation to undifferentiated mES cells.Results A total 86 differentially expressed genes,both up- and down-regulated,were found during the whole differentiation stage,6 gene clusters with the same developing patterns and differentially expressed genes in 6 groups were collected.By number,the most differentially expressed genes corresponded to posterior foregut stage( 201 genes) followed by definitive endoderm stage ( 17 genes).Conclusions The analysis of the genes with the same developing pattern and differentially expressed genes at each differentiation stage during pancreas development by microarray analysis provides some evidences of early embryonic and pancreatic development.

Objective:Study the relationship of HLA I and B7 1 to elucidate the role of costimulatory molecule on antigen presentation in immunological rejection.Methods:FCM was applied to detect the expression of B7 1 Then the cytotoxicity of PBML was observed before and after blocking the HLA I Results:The level of HLA I expression increased greatly(P

Objective To analyze the influencing factors of hemoglobin A1c in type 2 diabetic patients,including blood glucose,anti-diabetic therapeutic regiments,disease course,age,diabetic education,degree-educated,self-monitoring blood glucose.Methods Seventy-eight type 2 diabetic patients were divided into two groups by different degree of blood glucose,anti-diabetic therapeutic regiments,disease course,age,diabetic education,degree-educated,self-monitoring blood glucose.The levels of hemoglobin A1c in two groups were compared.Results There were significant changes in the levels of hemoglobin A1c between fasting blood glucose (FBG) ≤ 6.1 mmol/L group (30 cases) and FBG ＞ 6.1 mmol/L group (48cases),2 h postprandial blood glucose (2hBG) ≤8.0 mmol/L group (32 cases) and 2hBG ＞ 8.0 mmol/L group (46 cases ),insulin treatment group (27 cases) and no-insulin treatment group ( 51 cases ),combined drugs treatment group ( 36 cases ) and single drug treatment group ( 15 cases),age ≤ 65 years old group (41cases) and age ＞65 years old group (37 cases),disease course≤ 10 years group (39 cases) and disease course ＞ 10 years group( 39 cases),degree-educated above high grade group( 34 cases) and degree-educated below high grade group (44 cases),diabetic education ≥2 times/month group (20 cases) and diabetic education ＜ 2 times/month group (58 cases),self-monitoring blood glucose≥2 times/week group ( 19 cases)and self-monitoring blood glucose ＜ 2 times/week group (59 cases)[ (6.7 ± 1.5 )％ vs.(7.9 ± 1.3 )％,(6.8 ±1.1)％ vs.(7.8 ± 1.2)％,(6.7 ± 1.5)％ vs.(8.1 ± 1.4)％,(6.8 ± l.0)％vs.(8.0 ± 1.6)％,(6.9 ± 1.7)％ vs.(7.4 ± 1.6)％,(6.5 ± 1.2)％ vs.(8.2 ± 1.3)％,(6.9 ± 1.0)％ vs.(7.6 ± 1.4)％,(6.1 ± 1.7)％ vs.(8.0 ±1.1 ) ％,(6.7 ± 1.1 )％ vs.(7.6 ± 1.2)％ ] (P ＜ 0.01 or ＜ 0.05 ).Conclusion Blood glucose,anti-diabetic therapeutic regiments,disease course,diabetic,degree-educated,self-monitoring blood glucose are the important factors influencing hemoglobin A1c.

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Cardiac Surgical Procedures ; adverse effects ; Coma ; etiology ; psychology ; therapy ; Consciousness ; Female ; Heart Diseases ; surgery ; Humans ; Male ; Middle Aged ; Young Adult

Objective To investigate the endovascular treatment of peripheral vascular anomaly in children and its efficacy.Methods Eighty-three children with peripheral vascular anomaly were treated via endovascular means. Before and during the endovascular operation,all the cases were diagnosed as follows: arteriovenous fistula in 23 cases, cirsoid angioma 32 and cavernous hemangioma 28. The endovascular approaches included:①transcatheter embolization using coils or gelfoam sponge;② transcatheter embolization combined with local cirrhosis agents treatment.Results Immediate angiography after embolization showed all the arteriovenous fistulas and hemangiomas were efficiently occluded and the clinical presentation and symptoms improved greatly. Follow-up one to three years after operation demonstrated 100% of efficacy and 68.7% curative rate with no complications. Conclusions Endovascular approach for peripheral vascular anomaly in children is a micro-invasive, safe and effective way.

Clinical features of 2 cases of ectopic ACTH syndrome with manifestation of severe hypercortisonism are described with the aim of exploring effective therapies.

Livin is a new and important member of the inhibitor of apoptosis protein(IAPs)family.Livin is high expressed in most human tumor cells, involved in apoptosis inhibition and closely related with tumor development and progression.Studies found that through the down-regulation or inhibition of Livin expression and the induction specific antibodies of Livin could inhibi and kill tumor cells, which provided a new method for tumors'diagnosis, cell and gene targeted therapy.

ObjectiveTo observe the effect of Qishen Tongluo Zengzhi decoction on cognitive impairment in patients with acute phase of ischemic stroke.Methods A prospective randomized controlled trial was conducted, and 130 patients with acute phase of ischemic stroke and cognitive impairment accompanied by Qi deficiency and blood stasis and stagnationof phlegm-dampness syndrome admitted into the Neurology and Rehabilitation Departments of Rizhao Hospital of Traditional Chinese Medicine(TCM) Affiliated to Shandong University of TCM were randomly divided into treatment group and control group, 65 cases in each group. In the two groups, conventional internal treatment was given to all patients, and in the treatment group, additionally the Qishen Tongluo Zengzhi decoction was administered orally(composition: astragalus membranaceus 30 g, radix pseudostellariae 30 g, notoginseng 10 g,spatholobus stem 25 g, hirudo 3 g, pberetima 10 g, radix paeoniae rubra 12 g, Chinese angelica 12 g, peach kernel 10 g, carthamus tinctorious 10 g, achyranthes 12 g, radix rhapontici 10 g, rhizoma alismatis 6 g, Acorus gramineus Soland 9 g, polygala root 9 g, rhizoma cyperi 10 g, herba siegesbeckiae 15 g),one dose a day. While in the control group, oxiracetam 4.0 g intravenous drip was given, once a day. The whole course was 21 days in both groups. Before and after treatment, the cognitive function of all the patients in two groups was assessed by Mini-Mental State Examination(MMSE)and Montreal Cognitive Assessment(MoCA) scores, and incubation period and amplitude of P300 wave were recorded.Results Finally 62 cases were in treatment group and 63 cases in control group. Before treatment, the comparisons of the MMSE score, MoCA score and P300 latent period and amplitude between the two groups had no statistically significant differences(allP>0.05). After treatment in the two groups, the MMSE score, MoCA score and P300 wave amplitude were elevated, P300 latency period was shortened compared with those before treatment, and the changes were more prominent in treatment group〔score of MMSE: 25.33±2.32 vs. 21.68±2.29, score of MoCA(score): 26.61±3.06 vs. 22.40±2.93, P300 wave incubation period(ms): 349.62±20.01 vs. 371.87±19.63, P300 wave amplitude(μV): 8.70±2.92 vs. 5.72±2.33,allP<0.01〕.ConclusionQishen Tongluo Zengzhi decoctioncan effectively intervene cognitive impairment in patients with acute phase of ischemic stroke, and improve their cognitive function.

OBJECTIVE:To investigate the dosage and consumption sum of opioid analgesic drug in Qingdao district. METH-ODS:The consumption data of drugs in 29 public hospitals at secondary or above level in Qingdao district were analyzed statistical-ly by ABC analytic methods and defined daily dose methods. RESULTS:In ABC analysis,5 kinds of class A drugs accounted for 20% of the total number of species,and the percentage of consumption sum was 77.38%;4 kinds of class B drugs accounted for 16.00% of all species numbers,and the percentage of consumption sum was 11.98%;other 16 kinds of drugs accounted for 64.00% of the total number of species,and the percentage of consumption sum was 10.64%. Oxycodone sustained-release tablets and Morphine sustained-release tablets with the highest DDDs consumed more health care costs,serial number ratio was 1.00,syn-chronization was good and conform to the actual needs of clinical work. CONCLUSIONS:The composition ratio of opioid analge-sic drug costs is consistent with the theoretical value,significant discrepancy between cost and DDDs does not appear.