Objective:To search the impact of helicobacler pylori infection in changes of mice gastric mucosa mast cells and degranulation process,therefore to establish the important role of Hp in the pathogenesis of chronic urticaria.Methods:50 BALB/c healthy mice were randomized into two groups:Hp group,consist of 30 mice,were given Hp liquid every 3 days for total 7 times.Bovine albumin serum(BAS)as control group,was given BAS 2% with the same way as Hp group.Toluidine blue staining of the gastric mucosa was used to assess the total number of mast cells,the amount of degranulated mast cells and the percentage of degranulation after feeding.Each slide was cnnseeutive count with 10 high-power fields for mast cells; results express with number of mast cells/high power field,the ratio of degranulated mast cell was expressed by the amount of degranulated mast cells/total of mast cells.At the same time compared with the control group.Results:There was significant statistical difference in the total number of mast cells between the tow groups(100.0vs0.7,P

Objective To evaluate the effect of Huatan-Tongfu decoction in the treatment of subarachnoid hemorrhage (SAH) with heat-phlegm and sthenic-fu syndrome.Methods The SAH patients met the inclusion criteria were randomly recruited into a control group and a treatment group, 41 patients in each group.The control group was treated with western conventional medicine and sustained administration of nimodipine injection;the treatment group was nasal feeding or oral taking Huatan-Tongfu decoction on the basis of the control group.Both groups were treated for 14 days.On respectively 3, 7, 14 days after treatment the level of subarachnoid hematocele was evaluated with modified Fisher gradehemorrhage, the degree of serum nitric oxide levels was detected with ELISA method, and the clinical efficacy was evaluated according to the neurological impairment score.Results The total effective rate of 3 d (46.3％ vs.36.6％, P=0.006), 7 d (58.5％ vs.43.9％, P=0.000), 14 d (73.2％ vs.58.5％, P=0.000) in the treatment group were better than the control group (P＜0.01).After treatment 7 d (22.93 ± 7.32 μmol/L vs.16.52 ± 5.79 μmol/L, P=0.034), 14 d (27.65 ± 5.06 μmol/L vs.19.54 ± 3.76 μmol/L, P=0.015) serum nitric oxide levels in the treatment group were higher than in the control group (P＜0.05).After treatment, the improved Fisher scoring (1.29 ± 0.23 vs.1.69 ± 0.38, t=5.766) in the treatment group was significantly lower than the control group (P＜0.01).Conclusion Huatan-Tongfu decoction can improve SAH with heat-phlegm and sthenic-fu syndrome, relieve neurological defect function, reduce levels of NO, and improve clinical efficacy.

Objective To analyze thoroughly the related dates in internal and external hospital through data warehouse system.The valuable information is obtained to provide accurate and user-friendly decision support for the hospital.Methods The overall framework of data warehouse system and its key aspects are discussed with using Analysis Services tools for the design of the specific data warehouse system.Results To the HIS data sources and the specific needs of hospital data warehouse,many kinds of themes are designed including the patients component analysis,the component analysis of patients cost,the single disease analysis,cost-benefit analysis and comparative analysis of costs over the same period.Conclusion The data technology has reference value that promotes the application of data warehouse theory in hospital.

Vascular cognitive impairment (VCI) is a large class syndrome caused by a variety of cerebrovascular diseases from mild cognitive impairment to dementia.In the VCI animal experiments,choosing a reliable test method is particularly important for evaluating animal cognitive function and the improving the quality of animal models.

Objective To investigate the clinical efficacy of acupuncture plus Huayu Paishi Decoction (stagnation- eliminating and stone-removing decoction) in treating nephrolithiasis of qi and blood stagnation type.Methods Sixty patients with nephrolithiasis of qi and blood stagnation type were randomly allocated to treatment and control groups, 30 cases each. The treatment group received acupuncture plus oral administration of Huayu Paishi Decoction and the control group, oral administration of Huayu Paishi Decoction alone. The symptoms and signs were scored and renal hydrops was measured in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results The total efficacy rate was 76.7% in the treatment group and 56.7% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in the symptom and sign scores in the two groups (P<0.05). There were statistically significant differences in pre-/post-treatment symptom and sign score difference values between the treatment and control groups (P<0.05). There was a statistically significant pre-/post-treatment difference in renal hydrops volume in the treatment group (P<0.05). There was a statistically significant difference in pre-/post-treatment renal hydrops volume difference value between the treatment and control groups (P<0.05).Conclusions Acupuncture plus Huayu Paishi Decoction is an effective way to treat nephrolithiasisi of qi and blood stagnation type. It can markedly improve the clinical symptoms and reduce renal hydrops in the patients.

Objective To explore the Analgesic effect of dezocine combining with different concentrations of sufentanil on patient-controlled intravenous analgesia in patients undergoing cesarean section.Methods 120 patients of cesarean section patients from January 2013 to December 2015 in our hospital were randomly and equally selected in to into group D1,group D2 and group D3,40 cases in each group.Patients in group D1 were given dezocine 0.3mg/kg+sufentanil 1.0μg/kg+tropisetron 10mg,patients in group D2 were given dezocine 0.3mg/kg+sufentanil 1.5μg/kg+tropisetron 10 mg,patients in group D3 were given dezocine 0.3mg/kg+sufentanil 2.0μg/kg+tropisetron 10mg.Visual analogue scale(VAS),ramsay sedation scale(RSS),the number of remedy analgesic medication and side effects were observed and recorded 1,4,8,12,24 and 48 hours after surgery.Results Compared with D2 and D3 group,visual analogue score of D1 group was significantly increased,and the difference were statistically significant(P<0.05).Compared with D1 and D2 group,the incidence of adverse reactions in group D3 was significantly higher than that in group D1 and group D2,and the difference was statistically significant(P<0.05).There was no statistically significant difference in sedation scores among the three groups.Conclusion Compared with other concentrations of sufentanil combined with dezocine,the effect of intravenous patient-controlled analgesia after cesarean section was good with dezocine 0.3mg/kg+sufentanil 1.5μg/kg+tropisetron 10 mg,and the incidence of adverse reactions is low.

Objective To explore the expression of merlin and cyclins and the nucleocytoplasmic shuttling of merlin in abiogenous vestibular schwannoma tissues. Methods Four paraffin imbedding vestibular Schwannoma samples were included in the study.The cyclins and merlin were analyzed by immunofluorescence at the same time,and the expression and location were synchronously observed with confocal laser microscopy(CLM) in a single schwannoma cell. Results Cyclins and merlin were perspicuously and synchronously presented in a single cell in vestibular schwannoma tissues.In early G1 phase of the tumor cell,merlin was mainly stained in the nucleus,followed by perinucleus and cytoplasm.In G1/S phase,merlin was concentrated in the perinucleus,less in the nucleus and cytoplasm.Merlin staining showed uniform distribution in the nucleus in G2/M phase,while was absent from the nucleus and concentrated in the perinucleus in late G2/M phase. Conclusion The expression of merlin and various kinds of cyclins can be synchronously presented in abiogenous vestibular schwannoma cells with immunofluorescence and CLM.Merlin is shuttling among the nucleus,cytoplasm and perinucleus along with the progression of cell cycle.

Objective To study the negative emotion regulation of sea-going seafarers,establish a regulation strategy questionnaire of sea-going seafarers' negative emotion and examine its reliability and validity.Methods Based on open-ended questionnaires and previous researches,the initial questionnaire was obtained.Randomly selected 188 sea-going seafarers,by adopting program analysis and factor analysis,the final questionnaire was determined.Tested 121 sea-going seafarers by using the final questionnaire,while 55 of them by using coping style questionnaire at the same time.After two weeks,47 of them were tested by final questionnaire again.The reliability and validity of the questionnaire were investigated during the research,and the criterion validity between the coping style questionnaire and regulation strategy questionnaire of sea-going seafarers' negative emotion was also studied.Results The factor analysis results showed that the questionnaire was composed of seeking for support,abreaction,inobservance,transfer and oppression.Cronbach α of each factors was 0.857,0.841,0.825,0.806,0.754 respectively;and Cronbach o of the entire questionnaire was 0.873.The split-half correlation coefficients were 0.697,-0.839 (P ＜ 0.01) ; test-retest reliability coefficients were 0.706,-0.822 (P ＜ 0.01).The correlation between subscales were-0.031,-0.459 ; and the correlation between subscales and total scale were 0.590,-0.806 (P ＜ 0.01).Criterion validity showed that despite for the abreaction,each factor was significantly correlated with the factors of coping style questionnaire.Conclusion The sea-going seafarers' negative emotion regulation strategy questionnaire has acceptable psychometrics properties on reliability and validity.

Isocitrate lyase (ICL) which plays a pivotal role in the glyoxylate cycle of mycobacterium tuberculosis(MTB) could be a potential target against mycobacterium tuberculosis.Deoxyribozyme(DRz) is a small single-strand,enzymatic DNA molecule.Assistant by sketch of secondary structure,appropriate and not appropriate target sequences of MTB ICL mRNA were predicted.Two DRzs were designed targeting above sequences respectively.To affirm feasibility of this kind of prediction,in vitro cleavage of MTB ICL mRNA segment by DRz-ICLcf and DRz-ICLcj were performed.The results showed that sketch of secondary structure could indeed predict potential target of DRz.This research will provide basis for developing DRz as a novel antimicrobial agent against MTB.

Objective To investigate the efficacy of patient-controlled epidural analgesia(PCEA)with ropivacaine plus fentanyl for postoperative analgesia after suprapubic prostatectomy(SPP).Methods Thirty-six ASAⅡorⅢpatients aged 62-78 yrs weighing 52-84 kg undergoing SPP were randomly divided into 3 groups with 12 patients in each group:groupⅠreceived PCEA with 0.12% ropivacaine and fentanyl 1?g?ml~(-1);groupⅡreceived PCEA with 2% ropivacaine and fentanyl 1?g?ml~(-1) and groupⅢreceived patient-controlled intravenous analgesia(PCIA)with morphine 0.2 mg?ml~(-1).Postoperative pain was assessed using VAS(0=no pain,10= worst pain).The degree of motor blockade(modified Bromage scale)was evaluated at 6,24,48 and 72 h after operation(T_(1-4)).Bladder spasm episodes were recorded.Side effects including nausea,vomiting and pruritus and the total number of RBC in rinse solution were also recorded.Results(1)Bromage score(0=no motor block,3 =inability to flex ankle joint)was significantly higher in groupⅠandⅡthan in groupⅢat T1(6 h after op.), but significantly lower in groupⅠandⅢthan in groupⅡat T_(2-4)(24-72 h after operation).(2)VAS score(0= no pain,10=worst pain)was not significantly different among the 3 groups without bladder spasm but significantly higher in groupⅢthan in other 2 groups during episodes of bladder spasm.(3)The number of bladder spasm episodes was not significantly different among the 3 groups within 6h after operation,but was significant larger in groupⅢthan in the other two groups during 6-72 h after operation.During 6-24 h after operation more bladder spasm attacks occurred in groupⅠthan in groupⅡ.(4)The incidence of side effects was significantly lower and the total number of RBC in rinse solution was significantly smaller in groupⅠandⅡthan in groupⅢConclusion PCEA with 0.12% or 2% ropivacaine plus fentanyl can effectively reduce the number of bladder spasm attack and postoperative bladder bleeding.Different concentrations of ropivacaine should be used for PCEA during different postoperative periods.