OBJECTIVE To investigate the infection status and drug-resistance of Ureaplasma urealyticum(Uu) and Mycoplasma hominis(Mh) isolated from female genitourinary tract.METHODS A total of 1890 female genitourinary tract specimens were collected from Jan 2003 to Dec 2006.The results of mycoplasma cultivation and susceptibility test were analyzed retrospectively.RESULTS Among 1890 specimens,there were 756 with positive mycoplasma cultivation and the positive rate was 40.0%.The positive ratio was the highest at the age of 21 to 40.From 12 commonly used antibiotics,the sensitivity to tetracyclines was the highest,and then was to macrolides.Four quinolones all showed the higher resistance,and their sensitivity rates were all below 50%.CONCLUSIONS The drug-resistance of mycoplasma in female genitourinary tract is greatly severe.The clinical doctors should think highly of it.

Objective To study the effects of propofol pretreatment on S100? and neurosecretion enzyme (NSE) of brain tissues with global cerebral ischemia-reperfusion in rat, and to evaluate the effects of propofol in protection of brain. Methods 30 SD male rats were randomly divided into 3 groups: sham operation group (group A, n=10); single cerebral ischemia-reperfusion group (group B, n=10); propofol pretreatment at 2h before ischemia group (group C, n=10), in which propofol (100mg/kg) was given intraperitoneally (ip) before ischemia. 24h after ischemia-reperfusion, the neuroethology scores were recorded and evaluated, and S100? and NSE in rat brain were determined. Results The neuroethology scores of group A were higher than those of group B (P

There are many etiologies, which can produce dysbacteriosis. Among these etiologies which have serious influence and are commone clinically is prolonged, large and abuse of antibiotics.The principles of prevention and cure for dysbacteriosis are presented as follows: A. Reason-able use of antibiotics: (1) Short-term treatment with small dose; (2) Whenver possible, using nar-rowspectrum and different broadsprectram antibiotics ; ( 3 ) Avoiding taking orally; ( 4 ) Selective decontamination. B. Recommending usage of bio-gen and its growth factors.

objective To evaluate the relationship between the pulse pressure and the cardiac geometric structure in patients with essential hypertension. Methods Three hundred and ninety-five patients,age ranging from 20 to 77 years,were enrolled into the study. Their clinical systolic and diastolic blood pressure (SBP/DBP) were all above 140/90mmHg. All the patients either were initially diagnosed as having essential hypertension,or confirmed after taking a the placebo for 2 weeks after stopping anti-hypertensive drugs. The cardiac geometric structure was determined by the echocardiography at the same time when the patients untook the 24 hours ambulatory blood pressure monitoring. Results The clinical pulse pressure was significantly different among the groups as divided by the age ( P

Objective The purpose of this study was to investigate the effect of different level of sex steroids (?-estradiol and progesterone) on the proliferation of uterus endometrial epithelial cells. Methods Rabbit uterus endometrial epithelial cells were isolated by digestion of trypsin and centrifugation. The specificity and homogeneity of the endometrial epithelial cells cultured in media containing ?-estradiol and progesterone were determined by immunofluorescence staining of cytokeratin monoclonal antibody and the flow cytometric analysis. The different concentration of progesterone (P4) and ?-estradiol (E2) were added in media, the proliferative capability of the endometrial epithelial cells cells in vitro was determined indirectly by MTT assay. Results High purity endometrial epithelial cells (96%) were isolated using centrifugation method. The endometrial epithelial cells were examined by immunofluorescence staining. The endometrial epithelial cells were positive stained by anti-cytokeratin antibody. Immunofluorescence staining demonstrated that cytokeratin was detectable in the cytoplasm of epithelial cells. The cell growth curve showed that the proliferation of endometrial epithelial cells was not significantly changed by the treatment of E2 (100nmol/L) or P4 (100nmol/L) individually, but a combination of 100nmol/L E2 and 10nmol/L P4 could promote its proliferation greatly (P

Neonatal inherited metabolic diseases are a group of metabolic disorders caused by singe gene defect to cause a series of clinical symptoms.Neonatal dried blood spots have the advantages of simple preparation,safety,good stability,and show strong practicability in different screening methods for inherited metabolic diseases.With the development of screening methods,more and more diseases could be diagnosed by screening.The emergence of tandem mass spectrometry and molecular biological techniques promote the newborn screening and automation for inherited metabolic disease effectively.Inherited metabolic diseases induce great harm to the newborn,which could cause not only system organs damage,but also lead to death.Therefore,early screening is important for patients' prognosis.

BACKGROUND:Hydrophobic acrylic foldable intraocular lens should be evaluated biologicaly with New Zealand rabbits as implant objects prior to clinical trial. OBJECTIVE:To observe the biological safety of hydrophobic acrylic foldable intraocular lens. METHODS: Twelve New Zealand rabbits were enroled. The right eyes were implanted with self-developed hydrophobic acrylic foldable intraocular lens (Shenyang Baiao Medical Device Co., Ltd., China) as experimental group, and the left eyes were implanted with Acrysof IQ SN60WF (Alcon, USA) as control group. RESULTS AND CONCLUSION: After implantation, there were no significant differences in the cornea, anterior chamber, implant position, posterior segment of eyebal between two groups. Tissue proliferation had no obviously difference between the two groups, and there were cornea and lens pouch inflammations. No macrophages and other inflammatory cels were visualized on the surface of intraocular lens, and fibrous tissues were found on the intraocular lens surface and in the haptics root. These findings suggest that the self-developed hydrophobic acrylic foldable intraocular lens has no difference from Acrysof IQ SN60WF widely used in clinic, and it has the biological safety.

Objective To investigate effect of combination of rosuvastatin and dual antiplatelet therapy on the serum CRP, cerebral vascular event recurrence rate and carotid artery plaque in cerebral infarction patients.Method 60 cerebral infarction patients were seleted and divided into the control group and the experiment group by different treatment(n=30).Two groups were treated by corresponding drugs.The serum levels of IMT, CRP, plaque area, plaque number and the cases of recurrent cerebral vascular events after 6 month were compared after treatment a month.Results Compared with the control group after treatment,the serum CRP were lower(P<0.05),the recurrence rate of cerebral vascular events were lower(P<0.05),the IMT value, patch area and the number of carotid plaques were lower(P<0.05).Conclusion Rosuvastatin and dual antiplatelet combination therapy has good clinical effect for cerebral infarction patients,and have the clinical guiding significance.

Objective To explore the clinical effects of sitagliptin alone or its combination with metformin on the patients with type 2 diabetes registered on their first clinic/hospital visits.Methods Eightytwo patients with type 2 diabetes who diagnosed with oral glucose tolerance test (OGTT),insulin test and glucagons stimulating C-peptide test from May 2011 to February 2012 in our hospital were randomly divided into two groups:the patients in control group took sitagliptin alone (control group,n =41) and the patients in experimental group took sitagliptin combined with metformin (experimental group,n =41).Eighty-two patients,after the mission,were given diabetic diet and moderate exercise therapy.Taking sitagliptin 100 mg,1 times/d;combined with metformin groups given sitagliptin 100 mg/d,metformin 250 mg,3 times/d.Six months later,the major indexes comparing between before and after treatment of weight,body Mass Index (BMI),glycosylated hemoglobin(HbA1c),OGTT,fasting plasma glucose (FPG),2 h plasma glucose (2 hPG),fasting insulin (FINS),2 h postprandial insulin (2 hINS),fasting C-peptide and 2 h postprandial C-peptide levels were measured to research the changes during the treatments.Results After treatments,all the indexes were decreased significantly,and the BMI,HbA1c,FPG,2 hPG,FINS,2 hINS,fasting C-peptide and 2 h postprandial C-peptide in the group with sitagliptin united with metformin were significantly lower than the group with sitagliptin((27.4 ± 1.2) kg/m2 vs.(27.9 ±1.5) kg/m2,t =-2.812;(8.1 ±1.2)％ vs.(8.8 ± 1.3)％,t =-2.953;(8.17 ±1.22) mmol/L vs.(9.22 ± 1.28) mmol/L,t =-4.940;(10.16 ± 1.68) mmol/L vs.(11.01 ± 1.62) mmol/L,t =-3.362; (9.22 ± 1.93) mU/L vs.(9.85 ± 1.59) mU/L,t =-2.212;(74.62±13.73) mU/L vs.(83.75 ± 13.12) mU/L,t =-5.235;(1.43 ±0.34) μg/L vs.(1.77 ±0.27)μg/L,t =-3.194; (6.80 ± 1.40) μg/L vs.(7.61 ± 1.64) μg/L,t =-4.480 ; P ＜ 0.05).Conclusion Sitagliptin is useful to treat type Ⅱ diabetes,plays important roles in improving patients' quality of life and insulin resistance,control of blood glucose,reduce the family and the burden of social health,and the effect will be more better when united with metformin.

Objective To investigate the effect of potassium iodide on the incidence of sporotrichosis, and provide the theoretical basis for the prevention of sporotrichosis. Methods Experimental mice were divided into 4 groups, group 1～3 were fed with water containing different doses of potassium iodide, and no potassium iodide was given to the control group. After two weeks, the fungi were inoculated intraperitoneally to all the mice. In the third to the eighth weeks, the mice were sacrificed every week in batches. The infection, pathological changes and mycological findings of the mice were observed. Results The incidence rate of experimental sporotrichosis was significantly decreased in the mice (P