The platelet membrance glycoprotein plays a key role in the adhesion,aggregation and release of platelet.It is the specific molecular marker for platelet activation.Many studies have confirmed that platelet membrane glycoprotein CD62P may be more sensitive and specific to reflect the occurrence of coronary artery development,CD62P level is positively correlated with the severity.A variety of traditional Chinese medicine can lower the CD62P level,inhibit platelet activation and the treatment play an important role in the treatment.

Serum anti-uveal autoantigen with coiled coil domains and ankyrin repeats (UACA) antibody titer was measured using ELISA in 32 patients with thyroid-associated ophthalmopathy ( TAO ) and 20 patients with Graves' disease (GD) without ophthalmopathy.The level of anti-UACA antibody in TAO group was higher than that in GD group [ (225.91 ± 144.21 vs 187.42 ± 46.77 ) pg/ml,P＜0.05 ],and especially higher in patients with ocular myopathy [ (254.35 ± 168.29 ) pg/ml ].It seems that anti-UACA antibody is the sensitive clinical marker of ocular myopathy in TAO patients.

Objectives: To study safety method of control of blood glucose in perioperation patients with malignant gastrointestinal tumor and diabetes. Methods: The twenty eight cases with malignant gastrointestinal tumor and diabetes were analyzed retrospectively. Results: The patients with diabetes were safely controled in blood and urine glucose during perioperetion.There were no significant changes in serum lipids.The excretions of urea nitrogen in urine increased 24 hours after operation.The body weights declined and plasma proteins decreased after operation. Conclusions: The nutritional support is necessary and of feasibility in patients with malignant tumor and diabetes during perioperation.It is a key step to control blood glucose by using insuline.The nutritional support can protect function and structure of oragans.

Chronic Disease ; Heart Failure ; diagnosis ; Humans ; Medicine, Chinese Traditional

Cardiomyopathy, Hypertrophic ; complications ; Fatal Outcome ; Heart Septal Defects, Atrial ; complications ; Humans ; Infant

Protein-protein binding is the basis of virus and host cell interactions. With the application of technology of studying protein interactions, more knowledge of replication and pathogenesis of hepatitis C virus (HCV) was acquired. Non-structure protein 5B(NS5B) of HCV is a kind of viral protein, which plays an important role in replication of HCV. However, the effect of NS5B is not clear. To investigate the biological function of NS5B, we performed yeast two hybrid to look for proteins in hepatocytes interacting with NS5B. We constructed NS5B bait plasmid by cloning the gene of NS5B into pGBKT7, then transformed it into yeast AH109(a type). The transformed yeast was mated with yeast Y187(? type)containing liver cDNA library plasmid in 2?YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing x-?-gal for screening. Thirty-three colonies were selected and sequenced. Among them, two colonies were new genes with unknown function. The preliminary successful cloning of gene of protein interacting with NS5B paved the way for the study of the physiological function of NS5B and its associated protein.

Objective:To produce a fusion protein(ICOS-Ig)between extracellular portion of human ICOS and Fc portion of mouse IgG 2a and study on its biological activity.Methods:cDNA encoding the extracellular domain of human ICOS was prepared by RT-PCR from the RNA of the stimulated human peripherial blood mononuclear cells. The Fc portion of mouse IgG2a was cloned by PCR from the vector that contains the sequence-encoding Fc portion of mouse IgG2a.Two resulting amplified PCR products were ligated into a secrection mammalian expression vector, pSecTag2/Hygro A. The recombined vector was transfected into CHO cells by lipofectamine2000.Results:RT-PCR demonstrated the integration and mRNA synthesis of fusion gene. ELISA and Western blot analysis showed the expression of ICOS-Ig and its molecular weight was between 43-66 kD, its concentration was 5-25 ?g/ml. FACS analysis assured that ICOS-Ig had ligand specific binding activity. Addition of ICOS-Ig to MLR resulted in inhibition of T-cell proliferation and IL-2,IFN-? secretion.Conclusion:The fused gene ICOS-Ig was constructed and expressed successfully. It had the bioactivity of inhibition of T cell proliferation and cytokine secretion.

Objective To study the cause, prophyl ax is and management of the complications of laser treatment for varicose veins of the lower extremity. Method From Sep 2003 to Dec 2004, 52 patients (65 limbs) underwent endovenous ablat ion procedures. The laser was introduced into the vein to achieve venous occlusi on. Results Postoperative complications included ecchymosis in 12 cases, skin burn in 14 cases, saphenous nerve injury in 7 cases, thrombophlebitis in 3 cases, and subcutaneous fat liqu idation and infection in 2 cases. Most of the complications subsided after sympt omatic treatment in 2 to 3 weeks. Conclusion Most complications of laser treatment for varicose veins are transient and self-healing.

Objective: To evaluate a self-rated health measurement scale-the revised version 1.0 (SRHMS V1.0).Method:2000 subjects, including university students, patients in the department of traditional medicine, patients with dermatosis and military retired cadre, received the assessment with SRHMS V1.0. Result: The test-retest reliability of the above four groups was 0.80-0.98. The Cronbach alpha was 0.93, equal-length Spearman-Brown was 0.74, Guttaman Split-half was 0.73. The correlation between each item and its dimension was high, that between item and other dimension was low; furthermore, the correlation between each dimension and the subscale it belong to was high, that between the dimension and other subscales was low. Factor analysis produced ten main factors that were consistent with the original theoretical structure about SRHMS V1.0. The correlation between total score of SRHMS and that of SF-36 was significant (r=0.53). People of different age group, of different marriage status, with different occupations or education background, of different economic class had different results in SRHMS V1.0, while there was no difference significantly between male and female, between with different races or residencies. Conclusion: SRHMS V1.0 has good psychometric quality and can be used in Chinese population.

Objective To improve the recognition of the 8p11 myeloproliferative syndrome(EMS).Methods Here a case with EMS was reported and the medical literature on this topic were reviewed. Results The patient was definitely dignosed and was treated with DA regimen. Conclusion EMS is a rare hematologic malignancy with poor prognosis and short life span. The small molecule tyrosine kinase inhibitors may bring hope in the future for patient with EMS.