Animals ; Apoptosis ; drug effects ; Capillaries ; pathology ; Fibrosis ; Fingolimod Hydrochloride ; Kidney Diseases ; metabolism ; pathology ; Kidney Tubules ; blood supply ; Male ; Propylene Glycols ; pharmacology ; therapeutic use ; Rats ; Rats, Sprague-Dawley ; Sphingosine ; analogs & derivatives ; pharmacology ; therapeutic use ; Vascular Diseases ; prevention & control

Child ; Evidence-Based Medicine ; Glomerulonephritis ; complications ; diagnosis ; therapy ; virology ; Guidelines as Topic ; Hepatitis B ; complications ; Hepatitis B virus ; Humans

Caspase 3 ; metabolism ; Child ; Female ; Glomerulonephritis, Membranous ; enzymology ; etiology ; pathology ; virology ; Hepatitis B ; complications ; pathology ; Hepatitis B virus ; Humans ; Male

Objective To study the reliability of clinic diagnosis of hepatitis B virus(HBV)-associated glomerulonephritis.Met- hods Renal biopsy was carded out in 103 cases with clinically-diagnosed HBV-associated glomerulonephritis. The diagnostic consistency and clinical difference were evaluated.Results Sixty nine cases with clinically-diagnosed HBV-associated glomerulonephritis were confirmed through renal biopsy, 29 cases were excluded from HBV-associated glomerulonephritis. The pathologic diagnosis and clinical diagnosis methods were highly correlated and has significant difference(P

BACKGROUND: Edema and systemic disorder of energy metabolism after spinal cord injury can induce a series of amplifying effect, which can deterio rate the injured level. Matrix metalloproteinase-9 (MMP-9) is able to digest the basal lamina and damage the close joint of capillary vessel by proteolysis,which induces blood-brain barrier breakdown and brain edema formation.OBJECTIVE: To investigate the spatial characteristic of the MMP-9 in acute injured spinal cord so as to investigate the effect of MMP-9 on secondary spinal injury.DESIGN: A completely randomized study.MATERIALS: The study was completed in the Department of Pathology,Medical College of Wuhan University. Totally 40 healthy male Sprague Dawley rats were divided randomly into control group and model group.Five rats in control group were treated with laminectomy. Other 35 rats in model group was used to establish model of spinal cord injury.METHODS: After anesthetized, complete spinal cord tissue was taken out. Tissue of T8.9 spinal cord was cut out and every 3 serial sections of each tissue were taken out randomly for immunochemical analysis of MMP9 expression. Spinal cord tissue at injured segment was taken with 5 rats at each time point. Five visual fields (×400) of every slice were gathered randomly in injured area (sham-operation region in control group), average absorbency was determined then.MAIN OUTCOME MEASURES: Expression of MMP-9 in the acute in jured tissue of spinal cord.RESULTS: Totally 40 rats entered the final analysis. The expression of MMP-9 was observed 1 hour in model group with the peak time of 1 to 2 days and also seen on 7 days. The MMP-9 positive cells were mainly expressed on ischemic neuron, vascular endothelial cells and neutrophilic granulocyte. MMP-9 was not assayed in the control group.CONCLUSION: The early expression of MMP-9 and the characteristics of spatial distribution after spinal cord injury play a key role in tissue edema and inflammatory cell infiltration.

Objective To investigate the extracellular regulated protein kinases (ERKs) pathway of hepatitis B virus(HBV) X protein(HBx) on glomerular mesangial cell(GMC) proliferation of rat and tumor necrosis factor(TNF)-? expression.Methods The HBV X gene was amplified by polymerase chain reaction(PCR),inserted into the eukaryotic expression vector PCI-neo and confirmed by restrict endonuclease digestion and sequence analysis.PCI-neo contained HBV X gene (PCI-neo-X) was transfected into cultured GMC via liposome.GMC proliferation,TNF-? and its mRNA expression were investigated in the condition of with or without U0126 in the culture media.HBx,ERK1/2 and phosphorynated-ERK1/2 (p-ERK1/2) expression in GMC were assessed by Western blot.TNF-? mRNA expression was assessed by semi-quantitative reverse transcriptase-PCR (RT-PCR).TNF-? level in supernatants was measured by enzymelinked immunosorbent assay(ELISA).GMC proliferation was assessed by methyl thiazolyl tetrazolium(MTT).Results HBx expression was found in transfected GMC,and became prominent at 36 and 48 hours after transfection whether with or without U0126 in culture media.TNF-? mRNA expression was significantly decreased in U0126 group compared with U0126-free group.TNF-? levels in supernatants in PCI-neo-X transfection without U0126 group were (189.0?18.1) ng/L at 36 hours and (172.3?24.3) ng/L at 48 hours after transfection,respectively.In contrast,TNF-? levels in supernatants with U0126 were (65.6?11.6) ng/L and (84.0?24.6) ng/L,respectively.The TNF-? le-vels in the latter groups were significantly lower than the formers (Pa

Objective To investigate the clinicopathological features of hepatitis B virus-associated glomerulonephritis(HBV-GN) in children.Methods Clinical and pathological features were analyzed and correlated in 73 cases with renal biopsy-confirmed(HBV-GN).Sixty-six boys and 7 girls aged from 1 to 14 years old were included in this study,accounting for 14.4% in children under-(going) renal biopsy in the same period.Results Nephrotic syndrome(NS) was the prodominant clinical manifestation(53/73,72.6%),followed by glomerulonephritis(15/73,20.5%) and hematuria with proteinuria(5/73,6.9%).Serologic markers of HBV were found in all cases.Among them,55 cases(75%) were positive for hepatitis B surface antigen(HBsAg),hepatitis B e antigen(HBeAg) and anti-hepatitis B core(anti-HBc),14 cases(19.2%)positive for HBsAg,anti-HBe and anti-HBc,2 cases positive for HBsAg and anti-HBc,1 child positive for HBsAg and HBeAg,and another only positive for anti-HBc.The most common pathological type of HBV-GN was membranous nephropathy(MN),which was found in 69 cases(94.5%),followed by membranoproliferative glomerulonephritis(MPGN) and mesangial proliferative glomerulonephritis(MsPGN),2 cases(2.7%),respectively.All cases with NS presented themselves with MN.The pathological grades were significantly correlated with clinical manifestations through double trend analysis(?~2=5.22 P=0.022).Conclusions MN is predominant in childhood HBV-GN,which mainly presented with NS.Their clinical patterns are somewhat correlated with pathological grades.