Objective To investigate the changes in serum leptin and adiponectin levels and their significance in patients with chronic hepa-titis B virus (HBV)infection.Methods One hundred and twenty -five patients with chronic HBV infection (44 HBeAg -positive cases, 31 HBeAg -negative cases of chronic hepatitis B,23 chronic HBV carriers,and 27 inactive HBsAg carriers)were recruited in the present study.Fifty -five healthy people served as the control group.Blood samples were collected at 8 to 9 AMof the next morning after overnight fasting.Blood glucose,blood lipids,renal and liver function parameters,fasting insulin,serum HBV markers,and HBV DNA concentration were determined,and HOMA -IR was calculated.Serum levels of leptin and adiponectin were measured by ELISA.Continuous data were expressed as mean ±SD,and Student′s t -test,analysis of variance,linear regression analysis,and multiple regression analysis were ap-plied in the analysis of all experimental data.Results There were no significant differences in serum levels of leptin and adiponectin be-tween patients with chronic HBV infection and the control group (P =0.480;P =0.321),even after stratification by gender (males:P =0.210 /0.895;females:P =0.404 /0.066).After patients with chronic HBV infection were divided into subgroups by HBeAg status,HBV DNA,or ALT level,there were no significant differences in serum levels of leptin and adiponectin between groups (leptin:P =0.820 /0.296 /0.212;adiponectin:P =0.268 /0.760 /0.224).Conclusion For patients with chronic HBV infection,infection status and viral replication level are not significantly correlated with serum levels of leptin and adiponectin.

Intestinal fibrosis is the complications of inflammatory bowel disease (IBD), intestinal myofibro-blast cells are the key to intestinal fibrosis .Intestinal myofibroblasts and its interaction with inflammatory cells play an im-portant role in IBD-related intestinal fibrosis .

Objective: To make further evaluation on the efficacy and safety of Chuan Shen Tong Injection (CSTI) on benign prostatic hyperplasia with blood-stasis syndrome. Methods: A self-contrast between pre-and post-treatment and multicenter trial was carried out in 2090 subjects treated with CSTI over 4 weeks. Results: The markedly effective rate of CSTI in controlling symptoms is 60.77 %and the total effective rate is up to 91.67 %. Conclusion: Chuan Shen Tong Injection is effective and safe in treating benign prostatic hyperplasia with blood-stasis syndrome.

Objective:To study the characteristic of energy metabolism in moderate and severe OSA before and after CPAP. Methods:The REE was measured before and after 5-8 week treatment by CPAP in OSA patients. Health staff of single obesity in the hospital were as control. Results:During sleep, the enengy-metabolism was increased significantly in OSA patients. After treatment by CPAP, it was decreased (P

M channel, a voltage-dependent potassium channel, has been found in a variety of neurons. It is activated near the threshold of neuron action potential,producing a primary K~+ current, namely the M-current. The M-current can be modulated by many neurotransmitters and hormones, which can influence neuron excitability, conduction and neurotransmitter release. This review discusses the signal transduction pathway from G_~ q/11 activation to intracellular calcium, membrane phospholipid and phosphokinase, and explains the mechanism of M-current modulation by neurotransmitters.

As a member of homeobox gene family,HOXA5 is expressed in many organs and can regulate gene expression,cell differentiation and morphogenesis.Its structure and (or) function abnormalities are closely related to the development of leukemia,breast cancer,brain hemangioma,hepatocellular carcinoma and so on.Researching the relationships between HOXA5 and tumors contributes to cancer diagnosis,treatment and prevention.

Objective To investigate the significance of combined detection of serum tumor markers in the diagnosis of lung cancer,and to select the most sensitive serum tumor markers.Methods Enzyme-linked immunosorbent assay was used to detect the serum tumor markers of 62 lung cancer patients and 62 healthy persons,who were neuron specific enolase enzyme (NES),carbohydrate antigen 125 (CA 125),cytokeratin 19 (CYFRA21-1) and carcinoembryonic antigen(CEA).Results The level of serum CEA,CA125,CYFRA21-1,and NES of the lung cancer group were all higher than those of the control group (all P ＜ 0.05).Among the four kinds of tumor markers,CA125 had the highest sensitivity(59.7％),which was followed by CEA(53.2％).The sensitivity of the four kinds of joint detection was 87.1％,the highest sensitivity of three markers joint detection was CEA + CA125 + CYFRA21-1 (87.1％),while that of the two kinds of joint detection was CEA + CA125 (75.8％).Conclusion There are high clinical significance in the diagnosis of lung cancer with four kinds of serum tumor markers,CEA + CA125 + CYFRA21-1 can be used as the best markers for joint detection of lung cancer with high sensitivity.

Objective To investigate the value of BISAP scoring system in predicting the severity of acute pancreatitis.Methods Medline,EMBASE,Science Direct,Springer link,CBM,Cnki,Wan fang and VIP database were retrieved by computer between January 2000 and March 2013,and articles of BISAP score system in predicting the acute pancreatitis were collected according to strict inclusion criteria.Quality assessment was made by QUADAS scale.Meta-Disc1.4 software was used to analyze the heterogeneity of included articles and perform quantitative synthesis,as well as calculate the pooled sensitivity and specificity,positive likelihood ratio,negative likelihood ratio,and draw the ROC curve,and the results were presented with 95％ CI.Results A total of 11 articles were included,and there were 7 Chinese articles and 4 English articles.According to QUADAS scale,there were 4 articles of A grade,5 articles of B grade,2 articles of C grade.There were 6 studies using 2 as BISAP cut-off value,and 9 articles using 3 as cut-off value,while 4 studies using 2 cut-off values.Pooled analysis showed diagnostic odds ratio of 8.03 (95 ％ CI 5.66-11.38) when cut-off value was 2,and diagnostic odds ratio of 7.49 (95％ CI 5.35-10.49) when cut-off value was 3.There were moderate heterogeneity in both groups (I2 =63.3 ％,P =0.018; I2 =56.1％,P =0.019).When BISAP cut-off value was 2,the pooled analysis of sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and area under curve (AUC) were 59％ (95％ CI56％-63％),82％ (95％ CI 80％-83％),3.50 (95％ CI2.96-4.14),0.45 (95％ CI0.36-0.56) and0.82; and when BISAP cut-off value was 3,the corresponding values were 44％ (95％ CI41％-47％),90％ (95％ CI 89％-91％),4.59 (95％ CI 3.31-6.37),0.64 (95％ CI 0.61-0.68) and 0.64.The former had a high sensitivity,low specificity and large AUC,while the latter had a low sensitivity,high specificity and small AUC.Conclusions The best cut-off value of BISAP in predicting SAP is 2.When cut-off value is 2,the misdiagnosis rate is low,and the false positive rate is in the acceptable range,which is suitable for clinical application.

Objective To explore the application and effectiveness of simulated patient role play in the inquiry and the medical record writing teaching.Methods Carrying out the teaching model of simulated patient role play in the Second Clinical Medical College of Southern Medical University to the undergraduate students of grade 2009 snd 2010 in diagnose inquiry and medical record writing training class.A self-designed student questionnaire mainly about atmosphere of class,study enthusiasm,Self-evaluation of learning efficiency,the effect of the medical record writing in-class training and so on was used to do the survey.On the other hand,questionnaire for teachers included students' performance,students' writing ability,etc.100 students and 50 teachers were investigated with the survey.The percentage of the options of each question on the questionnaire was calculated in order to analyze the new teaching mode.Results The random questionnaire survey showed that 85％ (85/100) of the students believe that the new teaching mode can activate classroom atmosphere and improve learning motivation.About 62％(62/100) of the students said medical record writing errors can be obtained and corrected timely in-class training.Most students in grade 2009 have better capacity in medical record writing than those students who did not contact with new teaching mode.Conclusion The teaching model of simulated patient role play in the analogue inquiry and medical record writing is efficient and feasible in medical education,which can improve students' practical ability and is expected to be applied in undergraduate medical education.

Eighty-seven patients with hyperthyroidism were enrolled in a retrospective study,and 35.6％ of them also suffered from pulmonary hypertension(PH).Advanced age and systolic or diastolic dysfunction of left heart were risk factors in the mechanism of PH associated with hyperthyroidism (all P＜0.05).However,autoimmune disorder was found as a non-related factor in the mechanism of PH(P＞0.05).