BACKGROUND:Deep venous thrombosis is one of the common and fatal complications of total joint arthroplasty.For our clinical practice,we require duplex ultrasound and venography to evaluate the presence of deep venous thrombosis,but the diagnostic tools have several disadvantages.OBJECTIVE:To explore the appropriate and non-invasive biomarkers in the early diagnosis of deep venous thrombosis.METHODS:With the key words of Thrombosis,DVT,Biomarker,Total Joint Arthroplasty,D-dimer,Factor VIII,Thrombin Generation,Fibrin Monomer,P-selectin,Inflammatory Cytokines,Microparticles,Leukocyte Count,Genetic factors,MicroRNAs,a computer-based search was performed for articles published in PubMed database from January 1995 to March 2015.After the initial screening,the reserved articles were further detailed,summarized and concluded.RESULTS AND CONCLUSION: Totaly 66 articles were colected.This brief review is used to analyze the mechanism of the biomarkers which are classified into three levels as folow: detection of blood coagulation,inflammation markers and molecular biology and genetics-based biomarkers.The review also showed a great need of economic and safer biomarkers for the clinic and provided the theoretical basis to diagnose and predict deep venous thrombosis in an early stage.

Objective: To investigate the effect of prenatal tobacco and alcohol exposure on stature and neural development of offspring. Methods: Female mice were exposed to tobacco smoke and alcohol drink from D6 of pregnancy to parturition. The early physical development, neurobehavioral and antioxidative functions were measured in offspring mice. Results: Compared with control, offspring subjected to prenatal exposure showed significant reduced body and organ weights as well as reduced gestational body weight gain. It also resulted in remarkable prolonged neonatal reflexes and reduced motive, coordinative and locomotor activities. Step-down and Y-maze tests revealed marked destroyed memory and learning ability. Futhermore, MDA in offspring brain cytosol and mitochondria increased significantly and the activities of SOD, GSH-Px, CAT and GST were decreased. Conclusion: Prenatal tobacco and alcohol exposures interfere with the early physical and neural development in mice, probably related to decreased antioxidative functions.

Objective To application of solution focused approach in the functional gastrointestinal disorders.Methods 64 hospitalized clients with functional gastrointestinal disorders were randomly divided into the observation group and the control group with 32 cases in each group.We applied the normal nursing intervention to the control group,while applied the solution focused approach to the observation group.The anxiety and depression score,score of coping way and gastrointestinal symptoms were evaluated using t test.Results The scores of anxiety and depression,coping way and gastrointestinal symptoms were better in the observation group than those in the control group.Conclusions Adoption of solution focused approach can relieve FGID sufferers'negative emotion,such as anxiety and depression,increase sufferers' coping capacity and symptoms,which demonstrate pivotal significance for rehabilitation of functional gastrointestinal disorders.

China ; Evidence-Based Medicine ; methods ; Humans ; Orthopedics ; methods

Objective To investigate the efficacy of warfarin combined with urokinase in the prevention of deep venous thrombosis after hip replacement. Methods 58 patients undergoing hip replacement in our hospital from October 2014 to October 2016 were randomly divided into the observation group and the control group, 29 cases in each group. The control group were treated with oral warfarin, the observation group was treated with warfarin combined with urokinase, two groups of patients were treated for 14d, the incidence of lower limb deep vein thrombosis, follow-up statistics of two groups of patients within three months after operation, the diameter and blood rheology changes before and after weeks of treatment of lower extremity. Results During the treatment and follow-up of 14d patients in the control group, the incidence of deep venous thrombosis was 20.67% significantly higher than the observation group 3.45% (P<0.05); after treatment, the two groups of patients with lower limb circumference difference was significantly reduced (P<0.05), and the patients in the observation group the thigh circumference, calf circumference difference was significantly less than the control group (P<0.05); after treatment, the two groups of patients with high blood viscosity, low blood viscosity, plasma viscosity and hematocrit were significantly lower than those before treatment (P<0.05), and the indicators of the observation group were significantly lower than the control group (P<0.05). Conclusion Warfarin combined with urokinase can effectively prevent the formation of deep vein thrombosis of lower extremity after hip replacement, improve the hypercoagulability of patients and reduce the circumference of the lower extremity, so it is worthy of clinical application.

Objective To study the protective function of resveratrol on radiation-induced small intestine injury and lethal effect in mice.Methods Mice were randomly divided into three groups:irradiation (IR) control,IR only,and IR + resveratrol.15 mice each group were irradiated on abdomen with 7.2 Gy γ-rays for cell lethal assay and 8 mice each group were irradiated with 6.5 Gy for small intestine injury assay.For the IR + resveratrol group,the mouse was given resveratrol by intragastric administration 24 h before irradiation and then was fed with resveratrol daily for 5 days.The control and IR alone groups were fed with placebo.After 30 days of IR,mouse survival rate was detected.For small intestine injury experiments,24 h after IR,the mice were terminated and the small intestines were treated with HE and immunohistochemical staining.Results Compared with the irradiation group,resveratrol increased mouse survival by 33.3％,decreased apoptosis in intestinal crypt cells (t =17.35,P ＜ 0.05),and increased Ki67 expression (t =13.62,P ＜ 0.05).Conclusion Resveratrol could protect small intestine injury from ionizing irradiation.

To introduce the prophylaxis methods and dealing experiences of the common malfunctions of electronic gastroscopy. To summarize the prophylaxis methods and dealing experiences of the common malfunctions of electronic gastroscopy in recent years. To do everything carefully in the prophylaxis of the common malfunctions could ensure the high definition and resolution of the electronic gastroscopy. To manipulate properly with effective methods of preventing and dealing common malfunctions in use of electronic gastroscopy can not only lower the cost of repair but also prolong the service life of the machine.

Aim To establish a rat aortic calcification sub-chronic model induced by vitamin D3 and nicotine.Methods The vascular calcification model was produced by vitamin D3(300 kIU?kg-1,im) plus nicotine(25 mg?kg-1,5 ml?kg-1,po).Rat body weight and blood pressure were monitored on a regular basis.Ratio of heart to body weight,plasma calcium,plasma phosphate content,vascular calcium content,Von Kossa staining,activity of alkaline phosphatase(ALP) and 45Ca deposition in aorta were measured as the index of calcification at the 8th and the 16th week.Results Compared with control group,the VDN group rats showed higher values of blood pressure from the 3rd week(all P

Objective To investigate MELD and Child-Pugh in the patients with decompensated liver cirrhosis.Methods 41 cases of death and 71 survivors were graded with MELD and Child-Pugh and compared.Results The deaths' MELD and Child-Pugh score was (17.93?8.22 )and (10.07?1.84),the survivors' was (11.18?6.54 ) and (8.04?2.09)(P=0.000).Among the deaths and survivors,MELD≤9 was 14.63% and 42.25%(P =0.003),MELD 20-29 was 26.83% and 8.45%(P=0.009),Child A was 21.95% and 56.34%(P=0.000),Child C was 29.27% and 5.63%(P=0.001).Conclusion The deaths' MELD and Child-Pugh score is higher than the survivors'.The MELD score can act as a disease severity and prognosis index for patients with decompensated liver cirrhosis.

Objective To study the optimum component proportion of media and the best fermentation conditions for the flocculatnt-producing strain B5. Methods Through single factor experiment to seek the best carbon sources, nitrogen sources and inorganic salts. Furthermore, through orthogonal test to select the best conditions for producing flocculants by strain B5. Results The best carbon source, nitrogen source and inorganic salt were glucose, (NH4)2SO4 and CaCl2, the content of glucose was 2.0%, the initial pH value of medium was 7.0, the temperature was 30 ℃, agitation rate was 160 r/min and culture time was 24 hours in flakes, 50 ml liquids (250 ml triangular flask), the inoculation volume was 0.2 ml. The flocculants produced by strain B5 under the best fermentation conditions has been furnished for multifarious waste water, the flocculating activities were from 88.4% to 97.2% and reached to the highest on the waste water of dairy farming. Conclusion The flocculants produced by the strain B5 is applicable to the waste water treatment.