Animals ; Electrochemical Techniques ; methods ; Gene Silencing ; Genes, Reporter ; Humans ; MicroRNAs ; blood ; genetics ; metabolism ; physiology ; Microarray Analysis ; RNA, Messenger ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction

Animals ; Chronic Disease ; Heart Failure ; drug therapy ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors ; adverse effects ; therapeutic use

Adenoviruses, Human ; Child ; Humans ; Orthomyxoviridae ; Respiratory Syncytial Viruses ; Respiratory Tract Infections ; virology

Inner ear malformations are anomalies linking to development insults at different periods of embryogenesis,which are common causes of congenital sensorineural hearing loss. The evaluation of pediatric sensorineural hearing loss mostly depends on high-resolution computed tomography and magnetic resonance imaging, which can excellently depict the temporal bones and inner ear malformations.
Ear, Inner ; abnormalities ; diagnostic imaging ; embryology ; Hearing Loss, Sensorineural ; congenital ; Humans ; Magnetic Resonance Imaging ; Temporal Bone ; abnormalities ; diagnostic imaging ; Tomography, X-Ray Computed

Objective: To study the expression of cyclin D1 and E in lung cancer. Methods: The immunohistochemical technique was used to detect the expression of cyclin D1 and E in 68 cases of non small cell lung cancer(NSCLC) and 21 cases of small cell lung cancer(SCLC). Results: The rate of cyclin D1 protein positive expression was 48.5% in NSCLC, 4.8% in SCLC,showing significant difference( P

Antibodies, Viral ; blood ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin G ; blood ; Metapneumovirus ; genetics ; immunology ; Paramyxoviridae Infections ; complications ; diagnosis ; Respiratory Tract Infections ; blood ; etiology ; pathology ; Reverse Transcriptase Polymerase Chain Reaction

Adult ; Altitude ; Female ; Heart ; physiology ; Humans ; Male ; Occupational Health ; Railroads ; Tibet

OBJECTIVE: To explore different conditions and buffers of antigen retrieval which affect the CK of SNIP on immunohistochemical staining results. METHOD: Dividing paraffin tissue sections of 11 patients into four groups. Using the Image-Pro Plus Image analyzer and taking five horizons for each section to calculate an average of 200 areas, measured standard optical density of the positive reaction areas. RESULT: It is divided into four groups: high temperature and high pressure citrate buffer retrieval, microwave EDTA buffer retrieval, microwave citrate retrieval, high temperature and high pressure EDTA buffer retrieval. The standard optical density of positive reaction areas respectively express: 0.324 ± 0.051, 0.325 ± 0.056, 0.303 ± 0.061, 0.365 ± 0.023. The rates of CK positive expression with high temperature and high pressure EDTA buffer retrieval is batter than other repairing groups in the same paraffin tissue sections (P < 0.05). CONCLUSION For the Pan of Sinonasal inverted papilloma, the method of high temperature and high pressure EDTA buffer antigen retrieval can achieve the ideal staining results? which is worth while to promote and maybe as a bet? ter guide of clinic work.
Antigens ; analysis ; Buffers ; Humans ; Immunohistochemistry ; methods ; Microwaves ; Nose Neoplasms ; immunology ; Papilloma, Inverted ; immunology ; Paranasal Sinus Neoplasms ; immunology ; Staining and Labeling ; methods

Aim The effect of capsule bushenyanshou (BSYS), a compound of traditional Chinesemedicine, on the immunopharmacological activities of mice was investigated. MethodsThe indexes of immunopharmacological activity, such as the clearance rate of charcoalparticles, the lymphocyte transformation and the content of serum hemolysin, were mea-sured. Results Capsule BSYS (400, 800 mg?kg-1, qd ? 12) markedly increased theclearance rate of iv charcoal particles and 1he lymphocyte transformation stimulated invivo by PHA in mice. In hydrocortisone -treated mice(15 mg? kg-1, sc, qd ? 5), capsuleBSYS significantly enhanced the content of serum hemolysin and the weights of spleenand thymus. The results also demonstrated capsule BSYS performed a sighted inhibition ofthe delayed type hypersensitivity in mice. Conclusion Capsule BSYS has the capacity ofimmunological intensification and regulation.

Objective:To observe the effect of replenishing the kidney and eliminating phlegm therapy on calciumlcalmodulin-dependent protein kinase Ⅱ(CaMKⅡ-?) activities of AD rat model,and explore the potential mechanism.Methods:AD model was established by injection of ibotenic acid(IBO) and 25-35 ?-amyloid peptide(A?25-35) into basal nucleus of Meynert.After injecting for two weeks,Bushen Huatan Decoction was administered by oral gavage of high,middle and low dosages.Huperzine A-Zhulin Antun tablets served as control drug.Normal group,model group and blank group were administered with isovolumetric saline water.After treating for four weeks,hybridization in situ was used to observe the change of CaMK Ⅱ in hippocamp.Results:The expression of CaMKⅡ-? in model group was more than that in normal group(P