Carcinoma, Papillary ; genetics ; Humans ; Mutation ; Proto-Oncogene Proteins B-raf ; genetics ; Thyroid Neoplasms ; genetics

Objective To analyze the safety and diagnostic value of ultrasound-guided biopsy with simple biopsy clip in children.Methods Under simple biopsy clip 52 cases were performed percutaneous renal biopsy.Then the curative rate and the incidence rate of complications were evaluated.Results Of 52 cases,49 cases(94.23%) gained enough renal tissue specimen for histological evaluation;Three failed cases occurred at early stage,nothing was found at later stage.The main complication was macroscopic hematuria occurred only once or twice after the operation and perirenal hematoma in 1.92% of the cases.No other severe complications were found.Conclusions The biopsy clip is convenient and sterile,and can guide the biopsy device exactly and safely.The use of 18-gauge biopsy gun and the automated biopsy device in children can decrease the complication rate,which can be easily performed.

Through literature review of acupoint electric characteristic (AEC) detection, some problems in the traditional method of AEC detection were found. The major reasons were lack of distinguishing the relationship between the acupoint and "skin on the acupoint" in the electric characteristics, which can not solve those objective and subjective interferential factors in acupoint detection. Therefore, the traditional acupoint detection method is not stable, and its repeatability is no good. The conception to design the probe of acupoint characteristic detection is put forward, which can give a real time and dynamic detection for the electric characteristics of the acupoint's various anatomic layers. Meanwhile, the circumstance of the body tissue is relatively stable, so the detection method can avoid those interferential factors.
Acupuncture Points ; Humans ; Meridians

OBJECTIVETo observe effects of acupuncture and electroacupuncture on antioxidant enzyme and Ca2+(-) ATPase activities and Ca2+ content in mitochondria of skeletal muscle cells in rats of acute swimming exercise and explore the mechanism of acupuncture in increasing sport ability.

METHODSForty male SD rats were randomly divided into 4 groups. The rats in the acupuncture group and the electroacupuncture group were treated respectively with acupuncture and electroacupuncture before acute swimming exercise. After the swimming exercise, the rats were killed and the skeletal muscle was taken, and then the indexes were determined.

RESULTSAfter acute swimming exercise, the GSH-Px and Ca(2+)-ATPase activities in the mitochondria of the skeletal muscle decreased significantly (P < 0.05) and Ca2+ content increased (P < 0.01) in the swimming group as compared with the control group; in the acupuncture group, SOD activity increased significantly (P < 0.05), GSH-Px and Ca(2+)-ATPase activities increased as compared with the swimming group (P < 0.05); Ca2+ contents in the swimming group and the electroacupuncture group significantly increased as compared with the control group (P < 0.01).

CONCLUSIONAcupuncture can protect cells from injury of acute sports and maintain the functions of mitochondria so as to delay fatigue, prolong working time of muscles and prevent muscles from damage.

Animals ; Electroacupuncture ; Mitochondria ; Muscle, Skeletal ; metabolism ; Rats ; Rats, Sprague-Dawley ; Swimming

OBJECTIVETo observe the dose-effect relationship of electroacupuncture of different current intensities combined with Morphine of different dosage on alleviating the rats' tibial cancer pain, and explore the possible mechanism, which could provide the experiment basis for alleviating the tibial cancer pain by electroacupuncture combined with Morphine.

METHODSOne hundred female Wistar rats were randomly divided into a normal group, a model group and eight treatment groups, 10 cases in each group. The rats in the treatment groups were treated by combined therapies of electroacupuncture of different intensities with 2 Hz /100 Hz dense-disperse wave on "Jiaji"(EX-B 2)and different dosage Morphine in 2 factor 3 level conditions, once a day for 6 days. The pain thresholds were observed before the treatment and 0 min, 1 h, 2 h and 5 h after the first treatment as well as after 3 and 6 times of treatments. The glial fibrillary acidic protein (GFAP) expression was determined by immunohistochemical method.

RESULTSThe rats' pain thresholds were significantly increased with electroacupuncture of 2 mA and 1 mA (all P < 0.01) on the 0 min, 1 h and 2 h of the first treatment, between which there were no significant differences (all P > 0.05). The pain threshold was still increased by electroacupuncture of 2 mA on the 5 h of the treatment (P < 0.01), while that of 1 mA failed to take effect (P > 0.05). After 3 and 6 times of treatments, both electroacupuncture of 2 mA and 1 mA had the effect of increasing the pain threshold (all P < 0.01), and the effect of 2 mA was superior to that of 1 mA (P < 0.05), had the synergistic effect with 5 mg/(kg x d) Morphine (P < 0.05). After 6 times of treatments, both electroacupuncture of 2 mA and 1 mA could inhibit the expression of GFAP (both P < 0.01), and there was no significant difference between them (P > 0.05). Both of 5 mg/(kg x d) and 2.5 mg/(kg x d) of Morphine, however, didn't bring about inhibition effect (P > 0.05).

CONCLUSIONThere is a does-effect relationship on electroacupuncture of different current intensity for alleviating the tibial cancer pain in rats. The electroacupuncture with 2 mA, which is better than that with 1 mA, has the synergistic effect with 5 mg/(kg x d) of Morphine. The electroacupuncture can inhibit the expression of GFAP to cooperate with Morphine for the purpose of alleviating the rats' tibial cancer pain.

Animals ; Bone Neoplasms ; complications ; genetics ; metabolism ; Electroacupuncture ; instrumentation ; methods ; Female ; Gene Expression Regulation, Neoplastic ; Glial Fibrillary Acidic Protein ; genetics ; metabolism ; Humans ; Pain ; etiology ; genetics ; metabolism ; Pain Management ; instrumentation ; methods ; Rats ; Rats, Wistar ; Spine ; metabolism ; Tibia ; metabolism

The electric characteristics of acupoints have been confirmed by researchers at home and abroad. Because the traditional detection methods are various (mainly are different positions of the reference electrode), and they are influenced by many affective factors, with unstable results and bad repeatability, so the studies of the electric characteristics of acupoints are limited. The acupoint electric characteristic sensing needle is introduced in detail from its design, detection methods and so on, which can detects changes of the acupoint electric characteristics of channels and acupoints in different healthy states real-timely, dynamically and continuously. It not only can avoid those affective factors, and also the detective results are relatively stable and the repeatability is also better.
Acupuncture Points ; Electroacupuncture ; instrumentation ; Humans ; Needles

OBJECTIVETo assess the response in THP-1 treated with Rv3671c protein in Mycobacterium tuberculosis (M.tuberculosis).

METHODSThe gene encoding Rv3671c protein of M.tuberculosis was cloned into pET-28a vector and then expressed in Escherichia coli. The Rv3671c was purified with Ni-NTA affinity and ion exchange chromatography. The detection of protein concentration was by Lowry method.THP-1 cell was stimulated with Rv3671c protein and cells were analyzed by Hochest staining under fluorescence microscopy to assay cell death (apoptosis and necrosis). TNF-α and IL-1β were detected by ELISA at each stimulating time.

RESULTSThe Rv3671c protein of M.tuberculosis was successfully expressed in Escherichia coli. The purity of recombinant Rv3671c protein was 95%, and the protein concentration was up to 0.4 mg/ml. The nucleus of THP-1 was isolated and necrosis-like under fluorescence when cells were stimulated by Rv3671c protein. The levels of TNF-α and IL-1β in supernatant were 19 000 and 16 500 pg/ml respectively, and were significantly higher than control cells with the levels of 2100 and 3800 pg/ml separately.

CONCLUSIONThe necrosis of THP-1 cells could be stimulated by Rv3671c protein of M.tuberculosis and it was probably associated with high cytokines TNF-α and IL-1β levels.

Bacterial Proteins ; pharmacology ; Cell Death ; Cell Line ; Humans ; Interleukin-1beta ; metabolism ; Macrophages ; cytology ; metabolism ; Mycobacterium tuberculosis ; genetics ; Tumor Necrosis Factor-alpha ; metabolism

OBJECTIVETo probe into the best parameter of electroacupuncture (EA) for treatment of inflammatory pain in the rat.

METHODSOne hundred and twenty Wistar rats were randomly divided into 12 groups, normal control group, model group and 10 EA groups including A1 B1 C1, A1 B1 C2, A1 B2 C1, A1 B2 C2, A2 B1 C1, A2 B1 C2, A2 B2 C1, A2 B2 C2 (A1 : 2 Hz, A2: 100 Hz; B1 : successive wave; B2: intermittent wave; C1: 0.1 mA, C2: 0.2 mA), A3 B3 C1 (4/20 Hz, disperse-dense wave, 0.1 mA) and A3 B3 C2 (4/20 Hz, disperse-dense wave, 0.2 mA). The rats of adjuvant-induced arthritis in all of the EA groups were treated by EA with selected different parameters once every day for 6 days. Pain thresholds and beta-endorphin (beta-EP) content in the local tissue of inflammation were used as indexes to compare analgesic effects of EA of different frequencies, waveforms and currents by orthogonal experiment design and other methods.

RESULTSThe optimized parameters of raising the pain threshold was: 100 Hz, 0.1 mA, intermittent wave. EA at 100 Hz was better than 2 Hz for increasing the content of beta-EP in local tissue of inflammation. The analgesic effect of EA at 4/20 Hz, 0.1 mA, disperse-dense wave on the inflammatory pain in the rat was not significant different with that at 100 Hz, 0.1 mA, intermittent wave (P > 0.05).

CONCLUSIONThe best parameters are 100 Hz, 0.1 mA and intermittent wave for EA treatment of inflammatory pain in the rat.

Acupuncture Analgesia ; Animals ; Arthritis ; metabolism ; physiopathology ; therapy ; Electroacupuncture ; Female ; Humans ; Pain ; metabolism ; physiopathology ; Pain Management ; Pain Threshold ; Random Allocation ; Rats ; Rats, Wistar ; beta-Endorphin ; metabolism

OBJECTIVETo construct a recombinant prokaryotic expression vector (plasmid) containing microneme protein 1 (MIC1) partial gene in toxoplasma gondii (T. gondii) ZS2 isolate. The gene was expressed in varied Escherichia coli (E. coli) after sequencing.

METHODSThe gene fragment coding MIC 1 from the genomic DNA of T. gondii ZS2 isolate was amplified by polymerase chain reaction (PCR). The gene was inserted to a prokaryotic expression vector pWR450-1 by digesting with restriction enzymes and linking reaction. The positive clone was screened on LB plates containing ampicillin and identified by restrictive enzyme digestion, PCR amplification and sequence analysis. The recombinant plasmid was transferred into E. coli TG1, JM109 (DE3) and DH5 alpha, and was expressed under the induction of IPTG. The expression products were identified by SDS-PAGE. The MIC1 gene structure was analyzed and compared in homology with the gene sequence of RH isolate using computer software.

RESULTSThe recombinant plasmid pWR450-1/MIC1, after cloning from acquired 471 bp MIC1 gene fragment and amplified from the genome gene ZS2, was complete homologous to the sequence of RH isolate, reflecting its highly conservative. The gene could be expressed as fusion protein with 70,000 in varied E. coli.

CONCLUSIONRecombinant plasmid pWR450-MIC1 was successfully constructed and could be expressed in different strains of E. coli, laying a foundation for research on its structure and function.

Animals ; Base Sequence ; Cell Adhesion Molecules ; biosynthesis ; genetics ; Cloning, Molecular ; DNA, Protozoan ; analysis ; Escherichia coli ; genetics ; Gene Expression ; Molecular Sequence Data ; Nucleic Acid Amplification Techniques ; Protozoan Proteins ; biosynthesis ; genetics ; Recombinant Proteins ; biosynthesis ; genetics ; Toxoplasma ; genetics

OBJECTIVETo explore the best pulse waveform of electroacupuncture for treatment of adjuvant arthritis (AA).

METHODSForty Wistar rats were randomly divided into 5 groups. The electroacupuncture groups of AA were treated with different pulse waveforms of electroacupuncture, respectively. Pain threshold, swelling degrees of joint, and contents of 5-HT, beta-EP and LEK in local tissues of inflammation were observed.

RESULTSAfter the treatment, pain thresholds of the electroacupuncture groups with different pulse waveforms were higher than that of the model group (P < 0.05, P < 0.01); the local swelling degrees of inflammation in the electroacupuncture group were higher than that in the normal group (P < 0. 01), but was lower than that in the model group (P < 0.05, P < 0. 01); the contents of 5-HT in local tissues of inflammation in the electroacupuncture groups were lower than that in the model group (P < 0.05, P < 0.01); in comparison with the model group, the electroacupuncture of sound-electric wave increased the contents of beta-EP and LEK (P < 0.01, P < 0.05) and the electroacupuncture of intermittent wave increased the content of beta-EP in local tissues of inflammation (P < 0.05).

CONCLUSIONSound-electric wave is the best pulse waveform of electroacupuncture for treatment of adjuvant arthritis.

Acupuncture Analgesia ; Analgesics ; Animals ; Arthritis, Experimental ; therapy ; Electroacupuncture ; Humans ; Rats ; Rats, Wistar