Objective: To explore and practice the method of quality management of software about medical apparatus and instruments so as to ensure the quality of software. Methods:The quality management system of software developed from medical X-ray imaging equipment was constructed through two aspects included target management (quality measurement of qualitative and quantitative were adopted to ensure the quality of software could achieve predictive target) and process management(included the development management of Scrum mode, change management of process and version control based on Git technique. Results: After this method of quality management of software about medical apparatus and instruments was implemented, the software quality and satisfaction of customer were obviously enhanced. Conclusion: Through the control and management of two aspects, the target for ensuring and enhancing quality of software was achieved.

ObjectiveTo determine the clinical effect and the prospect of cervical side-to-side stapled esophagogastric anastomosis. MethodsEighteen cases of upper and middle thoracic esophageal carcinoma were treated with esophagectomy and cervical side-to-side stapled esophagogastric anastomosis. The clinical data were collected and retrospectively analyzed. The average age of patients was 60.7 years and the ratio of male to female was 17∶1. The tumor included 4 upper part and 14 middle thoracic parts. The length of tumors was from 1 cm to 7 cm, with an average of 3.2 cm.ResultsThe post-operative TNM staging(AJCC) included 4 Stage Ⅱa,9 Stage Ⅱb,and 5 Stage Ⅲ. The operative incisions included 8 nontransthoracic cervical and abdominal double incisions, 9 right cervico-thoraco-abdominal triple incisions, and 1 left cervico-thoracic incision. One (5.56%) patient complicated with anastomotic leakage and was cured in 2 weeks by cervical drainage and absolute diet. All the 18 patients were followed up for 1 to 5 years,and no anastomotic stricture was found. Reflux esophagitis was found in 2 and the incidence rate was 11.11%.ConclusionCervical side-to-side esophagogastric anastomosis is a safe procedure with larger resection of margins and fewer complications. It is worth replicating.

Objective:To explore the clinical correlation of HLA-DRB1 shared epitope with IgA mesangial proliferative glomer-ulonephritis( IgA MsPGN).Methods:The renal function were examined routinely,and HLA-DRB1 shared epitope were determined by PCR in a total of 164 patients with IgA MsPGN and 164 healthy subjects.The renal function and HLA-DRB1 shared epitope were compared in patients with IgA MsPGN and healthy subjects,and clinical correlation of HLA-DRB1 shared epitope and renal function were analyzed.Results:(1)24 h proteinuria,serum creatinine and serum urea nitrogen were significantly different in patients with IgA MsPGN and healthy subjects(P<0.01);(2)The high-frequency gene of HLA-DRB1 in patients with IgA MsPGN were DRB1*04, DRB1*07,DRB1*09,DRB1*11,DRB1*14 and DRB1*15;(3) The distribution frequency of DRB1*09 and DRB1*11 in patients with IgA MsPGN increased significantly(P<0.01 or P<0.05);(4)24 h proteinuria,serum creatinine and serum urea nitrogen in patients with IgA MsPGN with DRB1*09 and DRB1*11 were significantly different compared with those in patients with IgA MsPGN with other HLA-DRB1 shared epitope(P<0.01).Conclusion:HLA-DRB1 shared epitope is related to IgA MsPGN,DRB1*09 and DRB1*11 can increase the risk of IgA MsPGN,and related to the severity of glomerulonephritis.

The application of PET-CT could not only play a part in the diagnosis of clinical stage for NSCLC on PET, but also make full use of the discrimination of anatomical structure on CT. The precision of target contouring could be increased and the normal tissue and risk organ are also protected efficiently.

Background The signal pathway of mammalian target of rapamycin (mTOR) plays an important role in the regulation of cell cycle.Rapamycin(RAPA) can inhibit the proliferation of cells by regulating of cell cycle.Objective This study was to investigate the effect of RAPA on the proliferation of Rhesus retinal vascular endothelial cells (RF/6A).Methods RF/6A cell strains were cultured in vitro,and PBS,10 μg/L RAPA or 5 μg/L RAPA was added into the medium respectively.The expression of cyclin D1 protein in the RF/6A cells (absorbancy) were detected by Western blot.The cell cycle distribution after RAPA action was analyzed by flow cytometry.Matrigel was used in endothelial-cell tube formation to evaluate the effect of RAPA on angiogenesis.Results Western blot assay showed that the expressions of cyclin D z protein were 0.92±0.04,0.58±0.02 and 0.73±0.02 in the PBS group,10 μg/L RAPA group and 5 μg/L RAPA group,showing a significant difference among the three groups (F =246.320,P =0.000),and the relative expressing level of cyclin D1 protein in the l0 μg/L RAPA group and 5 μg/L RAPA group was significantly lower than that of the PBS group (both at P＜0.05).The proportion of G0/G1 cells were (42.13±0.57)％,(65.15±0.64)％ and (54.09± 0.78)％ respectively in the PBS group,10 μg/L RAPA group and 5 μg/L RAPA group,which was significantly different among the three groups (F=887.815,P=0.000).The number of endothelial-cell tubes were (9.67 ± 1.53)/field,(4.33 ± 0.58)/field and (6.33 ±0.58)/field respectively in the PBS group,10 μg/L RAPA group and 5 μg/L RAPA group,and the number of endothelial-cell tubes in 10 μg/L RAPA group and 5 μg/L RAPA group was less than that in the PBS group,with a significant difference among the three groups (F =21.778,P =0.002).Conclusions RAPA can inhibit the proliferation of RF/6A cells in vitro by down-regulating the expression of cyclin D1.

AIM:To investigate the corneal histopathology changes induced by soft contact lenses ( SCL) at diffent time.
METHODS: Central and peripheral cornea structures of myopic patients wearing SCL (150 cases) and the control goups ( 150 cases ) were observed with confocal microscopy. The examinations were performed 1, 3, 5, 8 and 10a after contact lenses wearing respectively.
RESULTS: After wearing the contact lenses for 5a, the cell density of corneal surface epithelial was lower in SCL wearers compared with control goups (P<0. 05) and the corneal vesicle and corneal gutata might be occasionally discovered. The density of Langerhans cells both in the central and peripheral cornea was higher in SCL group ( P<0. 05 ), and the tortuosity of the nerve fibers was significantly increased in the eye wearing SCL compared with control group. The densities of anterior stromal cells were lower while the microdot densities in the stroma were higher in SCL group than that in control group ( P<0.05 ) . The polymorphology of corneal endothelial cells was more obvious in SCL group, but the densities of endothelial cells were similar between two groups ( P>0.05). The SCL wearers showed a thinner thickness of central corneal epithelium than control group (P<0. 05).CONCLUSION: The corneal morphologic changes are more obvious with the longer time, and the changes is not ignored.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

Objective:To investigate the epidemic features of allergic rhinitis(AR)and correlative risk factors in Shihezi city,Xinjiang province,China. Method:A question-naire survey on AR and airway diseases was conducted among 2300 elementry school children in 12 schools,aged from 9 to 10 years old,from july 10 to 25,2008. in Shihezi city,Xinjiang province.Suspects of AR was identified by the survey,then they were recommended to specialist examinations for accurate diagnosis. Allergen skin tests were peformed to explore the allergenic varieties of allergic rhinitis. Diagnosis criteria of AR were that of ARIA(Allergic rhinitis and its impact on asthma). Resuit:Two thousand two hundred and eleven questionnaires were returned. The response rate was 96.1% and 2205 questionnaires were available.The prevalence rate of AR in children aged from 9 to 10 was 12.56%(277/2205) in Shihezi city.The difference of prevalence between female 13.76%(153/1112) and male 11.34%(124/1093) was insignificant statistically(χ~2=2.924,P＞0.05). The positive rate of the allergen skin test was 65.3%. The most common allergen of allergic rhinitis were plants of the mugwort and house dust mite. According to the ciassification criteria of ARIA in 2001,of 155 children suffered from intermittent AR,112 cases were mild and 43 cases were moderate-severe,while 122 suffered from persistent AR,110 cases were mild and 12 cases were moderatesevere. 5.4%(15/277) patients with AR also had bronchial asthma.25.3%(70/277) of the children with AR had inherent predisposition.Conclusion:The prevalence rate of AR in children aged from 9 to 10 in Shihezi city is 12.56%. Strategy of prevention and treatment of AR can be worked out according to the epidemical feature of AR.

Objective To explore the cognition processes of language in stroke patients by characterizing the differences between the event-related potentials (ERPs) N400 evoked bypicture-word andsentence-wordparadigms.Methods Twenty patients with a single brain lesion resulting from a first episode of stroke were tested along with 20 normal controls.Their N400 ERPs were tested using pictures and sentences as activating stimuli and phrases composed of nouns and verbs as the target stimuli.The N400 potentials were recorded from electrodes located at Fz,Pz,T3 and T4 positions of the International 10-20 system.The N400 latencies and amplitudes and the behavioral responses of the experimental and control groups were compared.Results The latencies were longer and the amplitudes were lower in response to sentence activation than to picture activation.The differences in terms of amplitude were the greatest with N400 recorded from Pz.The responses to the target stimuli were not significantly different with regard to the types of stimuli and recording location.The average response time was longer and the error rate was higher in response to sentence activation than to picture activation.Conclusion Multiple accesses,activating stimuli and integrating processes are involved in language cognition.The brain is more sensitive and accurate in response to picture stimuli in language cognition.The response to sentence stimuli is slower,and the error rate is higher.This suggests that it is better to usc varied prime stimuli in teaching children languages.