Objective:To explore the result of application of the clinical nursing pathway on percutaneous coronary interventional patient.Methods:One hundred patients were divided into two groups randomly,each group has 50 cases.We used the clinical nursing pathway based on holistic nursing case in experimental group,and the control group used the holistic nursing care.Results:After use the method of the clinical nursing pathway in the hospital,the medical cost and the rates of the nursing errors were less than that in the comparing group while the patients were more satisfied.Conclusion:As a scientific mode,the clinical nursing pathway could improve the working efficiency,guarantee the nursing quality.

Objective To explore the experience in the treatment of postoperative reocclusion in patients of lower limb arterial occlusion.Methods From January 2007 to December 2011,clinical data were reviewed on 98 reocclusion episodes in 70 patients,who had been treated by graft bypass (30 patients),or angioplasty (15 patients) or both (25 patients).In this series,10 cases received conservative treatment and to the other 88 episodes reoperation was given,including thrombectomy alone in 15 episodes,and thrombectomy combined with microcatheter application for 13 episodes,and thrombectomy combined with angioplasty and microcatheter application for 60 episodes.Results Two patients died during follow-up period and 5 patients were lost to follow-up including 2 receiving conservative treatment.The other 63 patients were followed-up for 8 to 60 months,averaging (24 ± 5) months.The arterial patency in patients receiving reoperation treatment was 69.8％ (44 patients) and the amputation rate was 12.7％ (8 patients).Conclusions For postoperative recurrent arterial occlusion in patients of lower limb arterial occlusion,thrembectomy combined with angioplasty and microcatheter application may generate satisfactory results,

Air Pollution, Indoor ; analysis ; Environment, Controlled ; Humidity ; Radon ; analysis ; Temperature

Objective To observe the effects of repetitive transcranial magnetic stimulation (rTMS) on the expression of glial fibrillary acidic protein (GFAP) and OX-42 in hippocampus and dentate gyrus in rats.Methods The rats were treated with 1 Hz, 100 mT TMS 10 min once a day for 14 days, and then the expression of GFAP and OX-42 in hippocampus and dentate gyrus were investigated by ABC technique of immunohistochemistry.Results Compared with the control group, there were no significant difference in the expression of GFAP and OX-42 between the two groups.Conclusion rTMS using our parameters does not cause brain injury in rats.

Mucosal adjuvants play important roles in vaccine development. By now, the common used mucosal adjuvants can be divided into three categories: the bacterial derivatives, cytokines and chemokines, and antigen delivery systems. Progresses of the three kinds of adjuvants were reviewed to give a reference to novel vaccine research.

BACKGROUND: The efficacy of Cyclosporin A(CyA) in the treatment of psoriasis is well recongized, the dosage less than 5mg/kg per day(mg/kg/d) is recommended. OBJECTIVE: The purpose of this study is to evaluate the indication, dose, course and side-effects of CyA therapy for psoriasis, especially in China. METHODS: Forty patients with psoriasis were treated with doses of CyA 5mg/kg/d, compared with 20 patients treated with Tigason lmg/kg/d by Global PAST. RESULTS: 1. Of 40 patients 33 patients (82.5%)were cured clinically in 4-5weeks and all the patients(100%) had responses to the therapy. 2. The efficacy and the Global PASI reduction of CyA therapy were significantly better than that of Tigason therapy. 3. During the CyA therapy, no severe side-effects and obvious laboratory changes were found. CONCLUSION: CyA(5mg/kg/d) was satisfactory for psoriasis, especially for psoriasis pustulosa, arthropathica and erythroderma. CyA was found to be superior to Tigason. The therapy course and side-effects seemed to be related to the dosage.
China* ; Clinical Study* ; Cyclosporine* ; Dermatitis, Exfoliative ; Etretinate ; Humans ; Psoriasis*

Objective:To analyze two different kinds of mouse model with immune thrombocytopenic purpura for the immune mechanisms.Methods:Immune thrombocytopenic purpura was induced by either injection of polyclonal antibody or immunized inoculation with rat platelets.Results:The two kinds of model all induced decrease of platelets,while immunization with platelets of rat induced alloantibodies in the mice.Conclusion:Immunization with platelets of rat can mimic the production process of autoantibodies in human and lead to immune thrombocytopenic purpura in mice.

Objective To study the influence of continuous blood purification(CBP)on the intra-abdomi-nal pressure (IAP ) and the gastrointestinal dysfunction of intra -abdominal hypertension (IAH ).Methods 80 patients with IAH were selected and divided into observation group and control group(n=40)according to random number table method.The observation group was given 3d CBP therapy.The control group was given conventional treatment.The mean arterial pressure (MAP ),IAP and abdominal perfusion pressure (APP ) were detected.The number of gastrointestinal symptoms was observed,sequential organ failure score(SOFA score)was used to assess the organ function of the two groups before and after treatment.Results The MAP,IAP,APP,SOFA score of the two groups before treatment had no statistically significant differences(P>0.05).The MAP of the two groups after treat-ment[(88.46 ±8.93)mmHg vs.(87.76 ±8.04)mmHg]had no statistically significant difference(P>0.05).The IAP[(9.02 ±1.06)mmHg]and SOFA score[(1.32 ±0.45)]of the observation group after treatment were signifi-cantly lower,the APP [(85.34 ±7.59)mmHg]was obviously higher than before treatment and the control group [(19.54 ±4.23)mmHg,(5.24 ±2.05)points,(70.76 ±6.43)mmHg],the differences were statistically significant (t=3.575,2.855,3.274,3.563,3.114,3.782,all P<0.05).Before treatment,the number of the gastrointestinal symptoms of the two groups[(44.56 ±8.53)times vs.(45.32 ±8.21)times]had no statistically significant difference (P>0.05).The number of gastrointestinal symptoms of the observation group after treatment [(6.54 ±2.24)times] was obviously lower than before treatment and the control group [(15.45 ±4.43)times],the difference was statisti-cally significant(t =3.022,P<0.05 ).The 30 d and 60 d survival rates of the observation group were 87.5%(35/40)and 65%(26/40),which of the control group were 80.0%(32/40)and 55%(22/40),although which of the observation group were higher than the control group,but there were no statistically significant differences(P>0.05 ).Conclusion CBP could significantly reduce the IPA of the patients with IAH,raise the APP,improve gastro-intestinal perfusion,reduce gastrointestinal symptoms,has positive significance to improve the gastrointestinal dysfunction.

ObjectiveTo explore serum interleukin-6 ( IL-6),interleukin-8 ( IL-8 ),tumor necrosis factor α (TNF-α) on neonatal hypoxic ischemic encephalopathy (HIE) in the pathogenesis of clinical significance before and after the intervention of neonates with asphyxia.Methodsforty cases of normal neonates were selected as normal group,70 cases of neonates with asphyxia were selected as asphyxia group and they were randomly divided into Fructose-1,6-diphosphate (FDP)group (conventional treatment combined with FDP,n =40) and control group ( conventional treatment,n =30 ).Patients in the conventional treatment group were maintained good ventilation function,their heart rate,blood pressure were in normal range,and their blood sugar were in normal high-value.And measures were taken to control convulsions,increase intracranial pressure and eliminate the brainstem symptoms. Patients in the Fructose-1,6-diphosphate (FDP) group were treated with intravenous FDP injection 250 mg/( kg · d)within 6h after birth in addition to the conventional treatment,and the course was 10 days.No special treatment was given to the healthy control group,and they returned to hospital for a return visit after discharged form hospital at the birth of the 10th day.The level of IL-8,IL-6 and TNF-α were monitored in three groups after they were born 6 h,3 d and 10 d,and 72 h HIE incidence rate were observed.Results There was no significant difference on the level of IL-6,IL-8 and TNF-α within 6 h after birth in asphyxia group ( P ＞ 0.05 ).Within 3 days after birth,IL-6 level was lower than that in the normal group [ (2.22 ± 1.26) ng/L vs ( 3.04 ± 0.98 ) ng/L,P ＜ 0.05 ],IL-8,TNF-α levels were higher than those in the normal group [ ( 18.62 ± 14.56)ng/L vs ( 15.62 ± 11.25 ) ng/L,(50.28 ± 18.74 ) ng/L vs (42.02 ± 12.25 )ng/L,P ＜0.05 ].In FDP group,within 3 days after birth,the level of IL-6 was higher than that in control group [ (2.65 ± 1.13 ) ng/L vs ( 1.24 ± 1.06) ng/L,P ＜ 0.05 ],the levels of IL-8 and TNF-α were significantly lower than those in the control group [ ( 17.24 ± 12.48 ) ng/L vs (22.46 ± 10.88 ) ng/L,(44.62 ± 18.27 ) ng/L vs (54.25 ± 17.28)ng/L,P ＜0.01 ] ;There was no significant difference on the level of IL-6 on the 10th day after birth among the three groups ( P ＞ 0.05 ).While in the FDP group,the levels of IL-8 and TNF-αwere significantly lower than those in the control group [ ( 14.25 ± 12.75 ) ng/L vs ( 17.23 ± 11.33 ) ng/L,(37.41 ±17.55)ng/L vs (48.21 ± 16.54)ng/L,P ＜ 0.01 ] ;The incidence of HIE and moderate to severe HIE in FDP group were significantly lower than that in the control group ( 22.5％ ( 9/40 ) vs 46.7％ ( 14/30 ),x2 =4.538,P＜0.05;5％(2/40)vs26.7％(8/30),x2 =4.595,P ＜0.05].ConclusionBlood level of IL-6 was lower and the level of IL-8 and TNF-α were higher in perinatal asphyxia and HIE patients,they may be involved in the pathogenesis of HIE.Early intervention can reduce the incidence of HIE and the incidence of severe HIE.