Diseases caused by dioxine include mainly various types of cancers and p53 is one important gene in carcinogenesis-suppressing gene family. Objectives: This research's goal is detecting p53 gene's ratio in the high risk group of exposure to dioxine. Methods: In this study we detected p53 ratio in 50 cases in the group of high risk of exposure to dioxine and the control group of 30 cases. Gene p53 was detected by PCR technique. Results: The study showed that in the control group (no exposure to dioxine), p53 prevalence is 100% compared with 82% in high - risk group (p <0.05). Conclusions: p53 was not detected in 18% of the group of high risk exposure to dioxin, suggesting that there were serious damages in p53 gene (deletion...)
Genes, p53

The second step of immunoglobulin gene alteration consists of somatic hypermutation and class switch recombination. 80th are regulated by activation-induced cytidine deaminase (AID). Methods: Study on possible application of class switch recombination assays for immunoglobulin gene alteration via AID. Cell based assays using AID B lymphocyte and NIH3T3 cell carrying switch substrate; gene transfer using retrovirus system; FACS analysis; PCR and ELISA. Results: DNA sequencing for S region and gamma1CT are the most sensitive and accurate assays. However, gamma1CT assay seemed to be more reliable and applicable. Others are accurate assays but less applicable. Conclusion: gamma1CT determination is the best class switch recombination assay for immunoglobulin gene alteration via AID.
Immunoglobulins, Genes

No abstract available.
Genes, Neoplasm*

No abstract available.
Genes, Neoplasm*

With the progress of molecular biology and the mapping of total human genome, treatment of monogene diseases by the method of gene engineering was possible as preliminary achieved success, but there were still unsolved problems. The most difficult problem had been that the insert of a healthy gene into a modified cell could product a deficit protein, but what is the mechanism of controlling and regulating its activity because homeostatic equilibrium is an important principle of a living body. This principle will not be assured when a gene activated without an equilibrium mechanism. When the new gene begins to activate, an other condition will be occurred, for instance an access of the previously deficit protein, i.e. an artificial cancer
Genes ; Therapeutics ; Disease

Using nested PCR with the specific primers for gag gene and the sequencing data from blood samples of HIV patients, we demonstrated that subtype E is also very common in the Northern area of Vietnam. This method can be applied for the cloning of HIV genes and molecular epidemiology of HIV-l in Vietnam.
HIV ; Genes ; Patients ; Classification

Rotavirus is the main cause of acute viral gastroenteritis in children under 5 years old. The study is to sequence nucleotides and amino acids of VP4, VP7, NSP1, and NSP4 genes of 5 passages of human rotavirus strain G1P8. The number of nucleotide mutations of VP4, VP7, NSP4 genes occuring among the passages were 3, 1, and 3, respectively. All these mutations resulted in changes in amino acid composition. No mutation was found in NSP1 gene.
Rotavirus ; Human ; Genes ; Child

. Genetic characteristics of two families having two children with Down's syndrome were analysed in the Department of Medical Biology and Genetics - Hanoi Medical University. Results: Family 1: Couple have two sons with Down's syndrome trisomy 21. Karyotype of the father 46,XY. Karyotype of the mother 46,XY Two sons (5 years and 3 years of old) have karyotype 47,XY,+21. Two sons have single horizontal fold on both hands. Family 2: Couple have a girl and a son with Down's syndrome translocated chromosome t (13;21). Karyotype of the father: 46,XY and of the mother 45,XX,-13,+t(13;21). Karyotype of the girl (12 years): 46,XX, 13,+t(13;21). Karyotype of the son (7 years) have: 46,XY,-13, +t(13;21). Two children have not any single fold on the hands.
Down Syndrome, Child, Genes

Study on cloning of the gene encoding human interleukin-2 with gene produce method by RT-PCR. Results: cloned and determine order of gene fragment encoded IL-2 that having 333pp size (133 amino acids) from total RNA of human spleen by RT-PCR. The results of analysis order of cloned IL2 gem showed that there were 2 changes of single nucleotide (A36G, G54T) in comparison with that of the NP-000577 order registered in NCBI GenBank A36G and G54T. However, these changes did not make changes in amino acid sequence of the cloned II-2 gene
Interleukins ; Human ; Genes

Study used the methods of PCR-SSCP-sequencing to detect and identify point mutations in pbp1a gene, one of the genes involved in penicillin resistance of streptococcus pneuminiae. SSCP analysis result of PCR products from 19 clinical resistant and 1 susceptible strains allowed their classification into defferent groups depending on their electrophoretic pattern. This classification fits results obtained by sequencing. With improvement in the resolution capacity of SSCP, these methods could be used efficiently to investigate penicillin resistance in streptococcus pneuminiae
Streptococcus pneumoniae ; Mutation ; Genes