Intravitreal injection of antiangiogenic agents is widely used to treat retinal vascular disease.This therapy can induce regression of neovascular vessels; reduce intraocular inflammation and retinal vascular permeability,and control macular edema.However the action period of these agents is short,and thus this therapy need repeated injections which cause higher operation risk and cost.Retinal laser photocoagulation therapy can close retinal capillary non-perfusion area and neovascular vessels,reduce macular edema caused by vascular leakage.However,as its therapeutic effect is based on the destruction of the retinal tissues in the lesion area,this therapy need longer time to show its effects.When the disease is controlled by this method,it may already induce some structural irreversible damages to the retina,especially the macular.This is why the visual acuity is not satisfactory in some patients,even though the disease get controlled,macular edema gets disappeared and anatomical structure of retina get improved.Properly evaluating all the pros and cons of retinal photocoagulation and intravitreal injection of antiangiogenic agents,will allow us to explore a better way to combine these two therapies to treat retinal vascular diseases.

The ocular fundus changes and the damage of visual function were various at different stages of diabetic retinopathy (DR). To get hold of timing and different therapic method correctly of early diagnosis, whole body treatment, laser photocoagulation and vitreous-retina surgery and adopting targeted interventions could help patients receiving the most reasonable and effective treatment at different stages,both of them are keys to reduce the damage of visual function.

Objective:To study the relationship between the cell density and the cell proliferation phenotype. Methods: Plate clonality assays was used to measure the impact of cell density to cell clonality and cell cycle in BT325、786-0、293、C6 and NIH3T3 cell lines. Results: The clonality decreased when the cells grown to confluence in NIH3T3 and 7860 cell lines respectively.It seem need more cells to decrease the clonality in 293 cell line but there is no relationship between cell density and cell clonality in BT325 and C6 cell lines.Cell cycle analysis show that cell density have no effect on BT325 and C6 but on 786-0、293 and NIH3T3 cell lines. Conclusion: There might exist preventer or preventers,which is proportional to the number of cells,of immortal stem cell to expand.In addition,the rate of stem cell expansion is proportional to that of cell mitosis in immortal cell lines.

OBJECTIVE:To evaluate the importance of monitoring serum concentration of Sodium Valproate(VPA)in the treatment of epileptic children.METHODS:Serum VPA concentration was determined by Chemiluminescence Enzyme Immunoassay(CLEIA),and the correlation between serum drug concentration and the daily dosage(mg?kg-1?d-1),dosage form,individual differences,blood sampling time and the clinical efficacy was analyzed.RESULTS:The serum VPA concentration which was lower than 50 mg?L-1 was found in 35.45% of the cases,and that which was higher than 100 mg?L-1 was found in 8.99%.CONCLUSION:The serum VPA concentration which was not in the therapeutic window can be resulted from multiple factors,and the monitoring results of the serum concentration should be analyzed comprehensively and made good use of.

Objective To investigate the effect of different doses of rosuvastatin Calcium in the treatment of coronary heart disease complicated with hyperlipemia and its effect on blood lipid level.Methods 100 patients with coronary heart disease complicated with hyperlipemia were randomly divided into the three groups according to the number table methods,the patients in group A received rosuvastatin Calcium 5mg/d,group B was given 10mg/d, group C was given 20mg/d.A treatment course was 4 weeks,and continuous treatment lasted 3 courses.The effects after treatment and serum TC,TG,LDL-C and HDL-C levels before and after treatment of the three groups were compared,and the occurrence of adverse reactions in the three groups of patients was recorded.Results The effective rate of C group in the treatment of coronary heart disease was 87.9%,the effective rate in the treatment of hyperlipi-demia was 93.9%,which were significantly higher than those in group A and group B (χ2 =6.54,P<0.05 );The serum TC,TG,HDL-C and HDL-C levels of three groups after treatment were significantly improved compared with before treatment,and which in C group were improved better than the other two groups(F=5.45,P<0.05);There was no significant adverse reactions in the three groups during treatment.Conclusion Large dose rosuvastatin Calci-um has a significant therapeutic effect in treatment of coronary heart disease complicated with hyperlipemia,which can effectively regulate blood lipid levels,with no obvious adverse reaction and high security,which is worthy of clinical application.

Objective To investigate the correlation between mechanical ventilation (MV) initiation time during cardiopulmonary resuscitation (CPR) and the prognosis of patients with in-hospital cardiac arrest (IHCA) in emergency . Methods Retrospective analysis of clinical records of patients with IHCA in emergency from January 2011 to April 2016 treated in Zhangye People's Hospital of Hexi University was performed. Patients with restoration of spontaneous circulation (ROSC) and were on MV with aged over 18 years were divided into early treated group (≤ 10 minutes) and later treated group (> 10 minutes) according to the initiation time of MV. Corneal reflex, pupillary reflex, pain-avoidance responses and motor response 24 hours after CPR, neurological function and cure rate of the two groups were analyzed. Results 210 patients were selected into our study including 130 males and 80 females (mean age: 60.24±13.17 years). There were no significant differences in gender, age, type of heart rate and etiological factor of cardiac arrest (CA) between the MV early stage group (124 cases) and the MV late stage group (86 cases). The restoration of corneal reflex, pupillary reflex, pain-avoidance responses, motor response and achievement ratio of CPR in early group were higher than those of later group (respectively, 59.68% vs. 31.40%, 59.68% vs. 31.40%, 54.84% vs. 24.42%, 54.84% vs. 24.42%, 70.16% vs. 51.16%, all P < 0.01); The D-dimer levels in the early group patients were significantly lower than those in the later group (μg/L: 478.39±57.21 vs. 510.05±62.83, P < 0.01). However, no statistical difference was observed between the two groups with respect to pH (7.24±0.72 vs.7.13±0.67, P > 0.05); The average hospitalized day of the early group was significantly shorter than that of the later group (days: 24.15±3.04 vs. 30.28±4.17, P < 0.01); Besides, the early group showed a higher survival rate at discharge and had more cases with neurologic level of grade 1-2 than those of the later group (Respectively, 41.94% vs. 26.74%, P < 0.05; 44.35% vs. 15.12%, P < 0.01). Conclusion Initiation MV on IHCA patients in the early stage of CPR (≤ 10 minutes) could help improve the hypoxic condition and prognosis of neurological function, and increase the achievement ratio of CPR.

Objective　To better understand the clinical characters of juvenile onset spondyloarthropa-thies (JSpA).Methods　The clinical and laboratory data of 190 in-patients with JSpA were analyzed and the diagnosis,classification and differentiation of juvenile onset arthritis were discussed.Results　Among these 190 patients,163 were male,with a male to female ratio 6∶1.Of them 92.1% had the disease after the age of 8.Peak of age at onset was 12 to 15 years;157(82.6%) patients had peripheral arthritis and only 23(12.1%) patients felt low back pain at onset.During the disease course, peripheral arthritis was found in 187(98.4%) patients and the history of low back pain or buttock pain was recorded in 123(64.7%).The interval between peripheral arthritis and low back pain was from 0 to 20 years,with an average of (3.2±4.5)years.Extra-articular features including enthesitis in 67(35.3%)patients,dactylitis in 20(10.5%),iritis in 9(4.7%) were observed.HLA-B27 was positive in 87.9%(160/182) patients.Sacroiliitis on X-ray was observed in 76.0%(136/179) patients,and 106(55.8%) patients were diagnosed juvenile ankylosing spondylitis (JAS) according to 1984 New York modified criteria.The average disease course in JAS was (6.3±6.2) years,longer than that in JSpA (P＜0.01).Conclusion　The concept of JSpA is helpful to early diagnosis and treatment of juvenile onset arthritis.The JSpA are characterized by asymmetric lower limb predominant oligoarthritis,a wide spectrum of extra-articular features,presence of HLA-B27 and familial history of SpA or psoriasis.It will take an average of 6.3 years for JSpA patients to fulfill the diagnostic criteria of adult AS.

Objective　To draw up the mental quality inventory for middle school students.Methods　Based on the content and level of normal mental activities and the scale analysis which investigates experts, teachers and middle school students, combining results of factor analysis, randomly sampled 5935 middle school students in Sichuan and Chongqing, this article analyzes reliability and validity, factor and correlation of the inventory in SPSS.Results　This article defines the mental quality of middle school students, constructs the structure and components of mental quality of middle school students by three dimensions of cognition, personality and adaptation (concretely, 22 factors), draws up the mental quality inventory for middle school students with good validity and reliability and formulates norm. Conclusion　Aiming at developing mental quality training and mental health education, the norm and the inventory have guiding significance in both of theory and practice.

Objective　To investigate the validity of the Chinese version of the Symptom Check-List-90 (the SCL-90) in Chinese population. Methods　A total of 624 normal person and 268 out patients were examined with the SCL-90. Results　（1）The internal consistency of the original subscales was found to be good and Cronbach's coefficient alpha ranged from 0.78 to 0.90; （2）Discrimination function analysis based on the 9 original subscales showed that the power of the SCL-90 in discriminating the patients and the community was good with a corrective rate of 80.6%; （3）Factor analysis on the items of the questionnaire yielded a very strong un-rotated first factor which could represent other factors; （4）High inter-correlations were found between the 9 original subscales of the SCL-90 with a correlation coefficient ranging from 0.39 to 0.79. Conclusion　(1) The SCL-90 is acceptable as a research setting and psychiatric screening inventory; (2) The SCL-90 does not have potential as an inventory to distinguish different diagnostic groups; (3) The SCL-90 is not a multidimensional inventory.

Objective To analyze the results of syphilis test and to understand the syphilis infection and its epidemiological characteristics .Methods 728 untreated patients with definite diagnosis of syphilis were enrolled and classified according to their age ,gender and syphilis types ,and retrospectively analysis was conducted .Results Among 728 syphilis patients ,446(61 .26% ) were diagnosed with latent syphilis ,211 (28 .98% ) with dominant syphilis ,32(4 .40% ) with neurosyphilis and 39(5 .36% ) with congenital syphilis .The average detection rate of syphilis was 1 .49% .332(45 .60% ) cases were found in female patients while 396 (54 .40% ) in male .Male syphilis patients were mainly in the 20 to 60 age group ,and female in the 20 to 50 age group .Dominant syphilis occurred mainly in the 20 to 40 age group and latent syphilis in the 20 to 60 age group .Conclusion The detection rate of syphilis increases year by year ,with the highest growing rate in latent syphilis and young adults as major incidence groups .