The preliminary results of inactivation of model parvovirus M13Mp 18 in plasma by long-waveUVA irradiation combined with psoralen derivatives were described.At 8-MOP concetration of300?g/ml plasma and UVA intensity of 11.5mW/cm~2,the UVA irradiation for 30～120 min couldresult in virus inactivation of 10~(5～9) infectious dose/ml.Quenchers were used to reduce the damage ofUVA to proteins in order to improve the clotting factor recovery after irradiation.2mmol/Lglutathione,or 2mmol/L glutathione with 2 mmol/L mannitol could significantly improve the Frecovery after irradiation.

Acupuncture Points ; Adult ; Diarrhea ; therapy ; Female ; Ginger ; chemistry ; Humans ; Irritable Bowel Syndrome ; therapy ; Male ; Middle Aged ; Moxibustion

ObjectiveTo survey the usage of prophylactic antimicrobial agents in type Ⅰ incision surgical operation.MethodsThe data of medication of 140 patients with type Ⅰ incision operation including thyroid surgery,breast surgery and repairing hernia surgery were investigated and statistically analyzed.ResultsThe preventative antibiotics usage in type Ⅰ incision operations was 100％.The frequency ranking of the perioperative antibiotics application was cephamycins ( 116 cases),cephalosporins ( 18 cases) and aminoglycosides ( 8 cases ).127 patients received antibacterials before operation.The total time for medication at least 1 day,up to 4 days.The antibacterials used in single kind and two kinds were 136 cases and 4 cases.ConclusionThe prophylactic use of antimicrobials in patients undergoing type Ⅰ incision operations in this hospital was irrational in the rate of antimicrobials use,choice of drug categories and medication duration,etc.Antibiotic prophylaxis in type Ⅰ incision perioperative should be further strengthened to supervise and management so as to promote rational use.

Objective To explore the clinical process and prognosis of middle and intermediate uveitis in children and adults.Methods A total of 121 patients with intermediate uveitis admitted to our hospital from January 2012 to December 2014 were enrolled in the study.Among them,there were 67 cases with monocular involvement and 54 cases with binocular involvement;59 cases were children and 62 cases were adults.All subjects were treated with personalized regimen according to the severity of the disease.The treatment effects and prognosis were compared between patients with different ocular involvement,children and adults.Results The total effective rate of treatment in patients with monocular involvement,children,patients with binocular involvement and adults were 92.5％,93.2％,92.6％ and 91.9％,respectively (all P ＞ 0.05).There was no significant difference in acute phase protein and visual acuity between the patients with ocular involvement and children,adults before treatment (all P ＞ 0.05),and there was no significant difference in the indexes between two groups after treatment (all P ＞ 0.05).The acute phase protein in each group after treatment was decreased,and the visual acuity was obviously increased,there were statistical differences before and after treatment (all P ＜ 0.05).Conclusion Individualized treatment regimen for patients with intermediate uveitis can improve the therapeutic effect,control inflammation and improve visual acuity.The curative effect is not significantly related to age and degree of ocular involvement.

Objective To investigate the effect of melatonin on serum malondialdehyde (MDA), NF-κB, tumor necrosis factor-α(TNF-α) andβ2-microglobulin (β2-MG) levels in secondary brain damage following intracerebral hemorrhage patients.Methods 90 patients secondary brain damage following intracerebral hemorrhage were divided into experimental group and control group, 45 cases in each group.The control group were given conventional treatment after surgical removal of hematoma, and the experimental group, on the basis of control group, were given melatonin capsule (6 mg melatonin) , orally, once a day, for 2 weeks treatment.The levels of MDA, NF-κB, TNF-αand β2-MG in patients’ serum were detected.Results The MDA, NF-κB, TNF-αand β2-MG levels after treatment significantly decreased compared with before treatment in two groups (all P<0.05), and the above indexes in experimental group significantly decreased compared with control group ( P <0.05 ).Conclusion Melatonin can significantly reduce the levels of MDA, NF-κB, TNF-αand β2-MG levels in brain injury patients with cerebral hemorrhage, and repair brain damage, which has a guiding significance for clinical use.

Background:14 C-urea breath test(14 C-UBT)is widely used for diagnosis of Helicobacter pylori(Hp)infection owing to its noninvasiveness and high sensitivity and specificity. Nevertheless,the presence of false-negative result can influence the diagnostic accuracy. Aims:To investigate the possible causes of false-negative 14 C-UBT for improving the accuracy of diagnosis. Methods:Eight-two cases with confirmed false-negative 14 C-UBT and 813 controls with true-positive 14 C-UBT at the People’s Hospital of Xinjiang Uygur Autonomous Region from Jan. 2014 to Aug. 2014 were enrolled. Patients in both groups were diagnosed as positive for Hp infection by Warthin-Starry silver staining combined with Hp stool antigen test. Univariate and multivariate analysis were performed to screen the factors related with false-negative 14 C-UBT. Results:In univariate analysis,5 variables were significantly different between case group and control group(P ﹤ 0. 05),and were taken into the multivariate analysis. Logistic stepwise regression analysis revealed that bile reflux( OR = 3. 961,P ﹤0. 001),post subtotal gastrectomy(OR = 9. 734,P ﹤ 0. 001),type Ⅱ Hp infection(OR = 1. 892,P = 0. 012)and upper gastrointestinal bleeding( OR = 4. 979,P ﹤ 0. 001 ) were the independent risk factors for false-negative 14 C-UBT. Conclusions:Bile reflux,upper gastrointestinal bleeding,post subtotal gastrectomy and type Ⅱ Hp infection might be the influential factors for false-negative 14 C-UBT. Combined tests for Hp infection should be suggested in patients with negative 14 C-UBT who had undergone subtotal gastrectomy or complicated with bile reflux or upper gastrointestinal bleeding.

Objective To study the effect of hydroxychloroquine (HCQ) on the suppressors of cytokine signaling (SOCS) in the kidney of MRL/lpr mice and evaluate the protection of HCQ on lupus nephritis.Methods Twenty-six rats were randomly divided into 3 groups:the control group which was consisted of 6 mice; the MRL/lpr group which was consisted of 10 mice; and the HCQ group which was consisted of 10.24-hour urinary protein level and anti dsDNA levels were tested.The expressions of SOCS-1 and SOCS-3 protein were determined by western blot.Analysis of variance was used to determine statistically significant differences between the two groups.A P value less than 0.05 was considered to be statistically significant.Results Compared with the control group,the levels of 24-hour urinary protein [(2 356.6±509.3) mg vs (440.3±90.1)mg] and anti ds-DNA (128.7±32.3 vs 14.9±1.4) were significantly increased in the MRL/lpr group,the expression levels of SOCS-1 and SOCS-3 protein were increased.After treated with HCQ,the levels of 24-hour urinary protein [(70.8±11.1) mg vs (2 356.6±509.3) mg] and anti-dsDNA (111.8±330.1 vs 128.7±32.3)were significantly decreased,the expression level of SOCS-1 and SOCS-3 protein decreased significantly (P＜ 0.05).Conclusion HCQ can inhibit SOCS expression in the kidney of lupus mice,reduce proteinuria and delay progression of immune disorders and lupus nephritis.

BACKGROUND:The power output of the human joints depends on the activation of agonists and antagonist muscles around the joints, and the antagonist muscle is involved in physical activity in a co-activated manner, thereby helping to maintain joint stability. But there are less reports on the central nervous system strategies of antagonist co-activation under different muscle contraction modes. OBJECTIVE:To observe the characteristics and differences of surface electromyography (sEMG) of agonists and antagonist muscles of the knee joints during maximal and submaximal isokinetic exercises until fatigue. METHODS:Sixteen students were enroled, who developed the right knee joint fatigue induced by the Biodex isokinetic equipment, and simultaneously sEMG activities of vastus medialis, vastus lateralis and biceps femoris were recorded by the myoelectrical equipment. The RMS (%) and mean frequency were selected to analyze sEMG characteristics. RESULTS AND CONCLUSION:In the maximal isokinetic fatigue, the peak torque was decreased by 40.3% (P < 0.05) and the sEMG activity of agonists gradualy increased and then declined, but the sEMG activity of antagonists kept constant. In the submaximal isokinetic fatigue, the peak torque was decreased by 40.0% (P < 0.05), the sEMG activity of agonists gradualy increased (P < 0.05), and however the sEMG activity of the antagonists initialy increased and then decreased. The RMS and mean frequency of vastus medialis and lateralis as antagonists at selected reciprocal contractions showed no significant changes (P > 0.05). These results suggest that in the different intensities of isokinetic fatigue, the central nervous system employs different neural strategies on agonists and antagonists.

The prevalence rate of diabetes is continuously increasing in China, and diabetes characterizes as high morbidity, low diagnostic rate, low cure rate, and low good control rate, which consequently resultes in the occurrence and progression of various diabetic complications and dramatically decreases the quality of life as well as the survival of diabetic patients.So it is crucial for clinician to screen diabetes early, make the diagnosis early, and start the treatment promptly.This article systemically summarizes the clinical manifestations, diagnostic criteria, typing, pathophysiology, complications, and management of diabetes.

[ ABSTRACT] AIM:To investigate the effect of sodium valproate ( VPA) on bleomycin-induced pulmonary fibro-sis ( PF) and its mechanism.METHODS: SD rats ( n =42 ) were randomly divided into model group and treatment group.Bleomycin at dose of 5 mg/kg was intratracheally injected to establish a rat PF model.The rats in treatment group were given normal saline (NS, 0.5 mL/d), VPA (300 mg· kg-1· d-1) or dexamethasone (DEX, 0.6 mg· kg-1· d-1 ) via intraperitoneal injection from the 14th day to the 28th day after modeling.The rats in model group were sacrificed on 0 d, 14 day and 28 d after modeling .The rats in treatment group were killed at 14th day after treatment.The effects of VPA on PF were evaluated by HE and Masson staining.The hydroxyproline ( HYP) content in the rat lung tissues was de-tected, and the expression ofα-smooth muscle actin (α-SMA) and E-cadherin was determined by Western blotting.RE-SULTS:HE staining showed that the alveolar structure and interstitial morphology in VPA group were better than those in NS group and DEX group.The level of collagen in VPA group was significantly lower than that in DEX group and NS group by determining the HYP content and Masson staining.VPA reduced the expression ofα-SMA, a mesenchymal marker pro-tein of PF, while increased the expression of epithelial marker protein E-cadherin.CONCLUSION:VPA reduces collagen content and distribution, and up-regulates the expression of the epithelial marker protein E-cadherin, while down-regulates mesenchymal marker proteinα-SMA, thereby preventing the rat lung tissues from bleomycin-induced PF.