Mitochondrial diseases is one of the common genetic metabolic diseases in children.The inheritance patterns are complex,both mendelian inheritance and mitochondrial maternal inheritance.Although the clinical feature is various and the diagnosis is difficult,but with the development of molecular biology,some clinical and genetic features in mitochondrial syndrome were gradually clear.This paper introduced the clinical feature and genetic characteristics in several common mitochondrial syndrome of children.

After the difficult position of hospital library and the experiences of Shanghai Public Health Clinical Center Library in meeting clinical and medical needs were described, such as relying on the advantages of Fudan library col-league, improving professional literacy, providing subject service, and expanding service contents, the author pointed out that providing excellent service through multiple channels and ways is the key for hospital library to improve its value and position and its sustainable development .

Autistic Disorder ; immunology ; physiopathology ; Humans ; Immune System ; physiopathology

Using brartemicin as the leading compound, fifteen novel trehalose derivatives were designed and synthesized, and the structures were determined by 11H NMR, MS and element analysis. Inhibitory effects of the target compounds on the proliferation of A549, HepG2 and HUVEC cells were detectec by MTT assay. The abilities of adhesion, invasion and migration of A549 and HepG2 cells inhibited by the synthesized compounds were evaluated through Matrigel experiment and Transwell assay. The results showed that, the target compounds had no significant cytotoxicity (compared with the control, P>0.05) to A549, HepG2 and HUVEC cells at the dose range of 1-32 µmol.L-1. At the above dose range, the inhibitory effects of A549 cells adhesion, invasion and migration and HepG2 cells adhesion and invasion by compounds 79 and 82 are better than brartemicin.
Antineoplastic Agents ; chemical synthesis ; pharmacology ; Cell Adhesion ; Cell Line, Tumor ; drug effects ; Drug Design ; Hep G2 Cells ; drug effects ; Human Umbilical Vein Endothelial Cells ; drug effects ; Humans ; Trehalose ; analogs & derivatives ; chemistry

Objective To establish the retrovirus expression vector containing glucose transporter-2 ( GLUT-2) gene and its stable virus producing cell line,for the construction of new "artificial islet beta cells". Methods Plasmid pCB7 /GLUT2 was digested by EcoRⅠ/BamHⅠ and cloned to retroviral vector PLXSN for the construction of recombinant plasmid pL-GLUT2-SN,which was identified by enzyme digestion and sequencing,and then transfected to packaging cell line PA317. Titer of the recombinant virus was detected. Cell lines with a high titer were cloned and identified by PCR and RT-PCR. Results The retrovirus expression vector pL-GLUT2-SN was established,in which the insertion site and reading frame of target gene were confirmed by enzyme digestion and sequence analysis. The highest titer of virus producing cell line PA317 /GLUT2 was 7. 1 ? 105 CFU/mL. PCR and RT-PCR showed that the GLUT-2 gene was integrated into PA317 /GLUT2 and stably expressed. Conclusion A retrovirus expression vector containing GLUT-2 gene and its stable virus producing cell line PA317 /GLUT2 are successfully constructed,thus laying a foundation for the construction of "artificial islet beta cells".

Objective To study the effect of Chinese medicine fumigation bath combined with acupuncture and hemorrhoids on improving the pain edema of elderly patients after anorectal pain.MethodsA total of 168 elderly patients with anorectal surgery were enrolled in our hospital from April 2013 to April 2016.The patients were randomly divided into two groups: the group (n=84) and the conventional treatment group (n=84) N=84).Routine treatment group given conventional Western medicine and potassium permanganate solution bath treatment, fumigation acupuncture group on the basis of conventional Western medicine treatment to give Chinese fumigation bath and acupuncture hemorrhoids treatment, compared the two groups of clinical efficacy, pain, Wound recovery and skin adverse reactions.ResultsThe total effective rate, no pain rate, edema disappearance time and wound healing time were 94.0%, 38.1%, (5.13±1.59) d, (16.47±4.06) d, respectively, compared with those in the conventional treatment group 84.5%, 22.6%, (6.74±1.23) d, (19.38±3.75) d, the difference was statistically significant (P<0.05).ConclusionChinese medicine fumigation bath combined acupuncture hemorrhoids acupuncture points can improve the local blood circulation, anti-bacterial anti-inflammatory pain, relieve senile patients after anorectal pain edema symptoms, speed up the rehabilitation process.

Hospital values in hospital management plays a leading position,the hospital values is the essence of hospital management and the core is embodied the spirit of the decisive factors in the hospital.Only thorough analysis of the reasons for the distortion of values and values distorted the adverse effects can be full demonstration Hospital values is the soul of hospital management.

0 05). Conclusion mtMSI may be involved in the carcinogenesis of some hepatocellular carcinomas, but mtMSI is not associated with nMSI.

Peroxisome proliferator-activated receptors(PPAR) are ligand-activated nuclear transcription factors which regulate the expression of many genes.Numbers of researchs indicate PPAR exert anti-inflammatory activities by inhibiting the expression of inflammatory singal transcription passage such as NF-?B,AP-1,STAT,meanwhile preserve from acute lung injury.This review will focus on structure of PPAR and their ligand and activity regulation and antiinflammation mechanism of action and protection to acute lung injury.

Post-transcriptional regulation of gene expression is an important contributor to disease pathogenesis.MicroRNAs (miRNAs) are endogenous, conserved, single-stranded non-coding small RNA with 18-25 nucleotides in length, which regulate gene expression post-transcriptionally.Several studies found that miRNAs could affect the development of nonalcoholic fatty liver disease through multiple ways.This article reviews the roles of miRNAs in the development of nonalcoholic fatty liver disease by regulating lipid metabolism, insulin resistance, oxidative stress, inflammatory, intestinal mucosal barrier, cell apoptosis and so on.