Objective To investigate the expression of Human leucocyte antigen(HLA)-DP,-DQ,-DR and CD40 in human retinal pigment epithelial(RPE)cells,to determine their molecule expression in immune response process,and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without(-IFN respectively.Expression of HLA-DP,-DQ,-DR and CD40 was measured by immunohistochemical staining.Meanwhile,peripheral blood mononuelear cells(PBMC)were co-cultured with RPE cells in vitro,and then the expression of activated lymphocytes CD69 was measured by fluorescence activated eell sorter(FACS). Results Expression of HLA-DP,-DQ,-DR and CD40 antigen were enhanced by γ-interferon inducement.Increasing amount of CD69 positive Iymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphocytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.

Objective To construct the clone vector of S gene in occult hepatitis B virus infection.To analyse its mutations and to construct its prokaryotic expression vector.Methods To amplify the HBV S gene with polymerase chain reaction(PCR),recombinate the S gene and vector PUC18,sub-clone the recombination and construct its prokaryotic expression vector with pThiohis B. Results There were two samples negative with positive HBV DNA from 64 samples with negative hepatitis B surface antigen.The clone vector and prokaryotic fusion protein expression vector of an occult HBV infection′s S gene were successfully constructed.The S gene was not changed by sequencing analysis.Conclusion The fluorescence quantitive polymerase chain reaction (FQ-PCR) can examine low-copies in occult hepatitis B virus infection.Determining quantitivly HBV DNA of recombination can identify the recombination and display multiplication state intuitirely.It is very successful in constructing the clone vector and prokaryotic fusion protein expression vector.

Objective:To explore the role of hypoxia in C_2C_(12) cells proliferation.Methods:We chose 10% oxygen concentration.Cell numbers were counted with a hemacytometer.The proliferous indexes were observed with a flow cytometer.HIF-1? protein was determined by Western blot,mRNA expression was determined with reverse transcriptase-polymerase chain reaction(RT-PCR).Results:The cell numbers and proliferous indexes were higher(P

Objective:To investigate the capability of corneal cells to stimulate lymphocyte proliferation and the expression of costimulatory molecules on primary human corneal epithelial cells.Methods:Human corneal epithelial cells were cocultivated with peripheral blood mononuclear cells(PBMC). Expression of HLA class Ⅱ antigens, CD40, CD154, CD80 and CD86 was measured by immunohistochemical staining,and the expression of such molecules above on rejected corneal grafts was contrastingly assessed. Activation of lymphocytes was determined by measuring upregulation of CD69 by FACS analysis.Results:HLAⅡ expression was detectable on human primary corneal epithelial cells,and furthermore, upregualtion of costimulatory molecules CD40 and CD80 were also found after induction with ?-interferon. Both of HLA and CD40 were expressed on rejected corneal grafts. T lymphocytes were activated by epithelial cells in the coculture system.Conclusion:Human corneal epithelial cells are involved in rejection process of the corneal transplantation. The immunological reaction is triggered by epithelial cells,and endothelial cells suffer as the targets.

Objective To study the expression of interleukin 6 (IL 6) and tumor necrosis factor ? (TNF ?) , location of both cytokines and their relationship with the variation of bone mineral density (BMD). Methods Cancellous bone chips were obtained from the vertebral bone of senile osteoporosis and control subjects during operation. Slides were made and stained with IL 6 and TNF ? monoclonal antibody, respectively. Results Osteoblasts and stromal cells were strongly positive to IL 6 and TNF ? in osteoporosis groups. The number of positive cells in high power field are 107 9?42 6 and 99 0?31 8 in osteoporosis groups, and 31 4?20 5 and 28 5?17 1 in controls, respectively, showing significant difference between two groups ( P

OBJECTIVE:To establish the method for simultaneous determination of the content of metronidazole,clindamycin hydrochloride,spironolactone and vitamin B6 in Compound metronidazole clindamycin cream.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-[0.68％ dipotassium hydrogen phosphate solution (containing 1％ triethylamine)] (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm,and column temperature was 25 ℃.The sample size was 20 μL.RESULTS:The linear ranges of metronidazole,clindamycin hydrochloride,spironolactone and vitamin B8 were respectively 20.0-120.0 i.tg/mL(r=0.999 9),10.0-60.0μg/mL(r=0.999 1),4.0-24.0 μg/mL(r=0.999 4),10.0-60.0 μg/mL (r=0.999 4).The quantitative limits were respectively 13.06,7.36,2.43,6.42 μg/mL.The detection limits were 4.18,1.82,0.76,1.79 μg/mL.RSDs of the precision,stability,repeatability tests were all less than 2％.The recovery rates were respectively 98.97％-100.91％ (RSD=0.79％,n=9),99.02％-101.80％ (RSD=1.03％,n=9),98.18％-101.05％ (RSD=1.01％,n=9) and 98.75％-101.36％ (RSD=0.87％,n=9).CONCLUSIONS:The method is simple,rapid,accurate and can be used for the determination of metronidazole,clindamycin hydrochloride,spironolactone and vitamin B6 in Compound clindamycin metronidazole cream.

The progress of globalization and frequent international exchanges in the medical com-munity demand doctors' improved English speaking competence, particularly in the clinical aspect. On the basis of the First Principles of Instruction, the research has proposed a module-based teaching model, which integrates 7 modules, including history taking, questions about frequency and pains, analysis of the possible causes, initial check-up, giving prescription, further investigation and conditional advice. The model is cen-tered on linguistic functions and aimed at helping students become proficient in speaking medically through the phrases of demonstration, application, reflection and activation. The model, after two years suc-cessive teaching practice among the elite class undergraduates, received a favorable feedback in triggering interests, classroom arrangement, material selection, and its compliance with FLA (foreign language acquisition), and was proved to be an effective way to improve medical students' English speaking ability in handling clinical dialogues.

Objective To study of relationship between elderly people's alexithymia and mind-reading. Methods There were 108 old people over 70 years old, tested by mind-reading,cognitive ability and twenty-item Toronto alexithymia scale. Results There were significant difference in mind-reading(16.00±4.84, 19.36±2.91,t =3.38,P ＜0.01), memory span(5.80±1.42,7.18±1.13,t =4.28,P ＜0.01), working memory( t =4.05,P ＜0.01),processing speed( t =-4.80,P ＜0.01) between high and low alexithymia groups.Alexithymia scores were negatively related to the scores of mind-reading, memory span,working memory,processing speed,correlation coefficient was 0.227( P <0.05),-0.377( P <0.01),-0.334( P <0.01),-0.470( P <0.01),respectively. With mind-reading,cognitive processing and educational level as independent variables,with total scores, F1, F2, and F3 of TAS as dependent variable,respectively,the regression equation of total scores was significant( F =8.594,P ＜0.01), and the one of F1 was significant( F =8.796,P ＜0.01),but the one of F2( F =1.735,P ＞0.05) and F3( F =1.181,P ＞0.05) was not significant. Conclusion Elderly people's alexithymia is negatively related to mind-reading and several cognitive abilities. Mind-reading could affect emotional recognition, but not affect emotional description and export-oriented thinking.

Objective To study the clinical effect of cohesive elastic bandage (CEB) after operative treatment of ankle fractures. Methods After surgery, 60 patients with ankle fracture were randomly as-signed to 2 even groups: CEB dressing group and common dressing group. Functional exercise started the next day after operation. Comparison was made between the 2 groups in drainage, displacement and swelling. Results In CEB dressing group, the volume of drainage was (34.6±18.1) mL, the rate of displacement 6.7%, and the swelling was slight in 21 patients, moderate in 5 and severe in 4. In common dressing group, the volume of drainage was (85.1±17.1) mL, the rate of displacement was 63.3%, and the swelling was slight in 7 patients, moderate in 12 and severe in 11. The differences were significance(P<0.05) . Conclusion Use of CEB after surgery for ankle fractures may alleviate swelling, reduce bleeding effectively, and benefit early functional exercise.

Objective To investigate the diagnosis and treatment of renal angiomyolipoma with hemorrhage. Methods Eighteen cases of renal angiomyolipoma with hemorrhage were diagnosed by imaging such as ultrasound and CT from June 2000 to December 2007.Open operations were adopted in 15 cases. Expectant treatment was adopted in 3 cases. Results Hemorrhage stopped in 3 cases with expectant treatment.All the operations succeeded.Pathological diagnosis after operations was renal angiomyolipoma.The follow-up time was 3 to 60 months.Ultrasound,CT and intravenous pyelography were mainly methods.There was no case of relapse or fistula.Tumor size of 3 cases with expectant treatment changed little. Conclusions Diagnosis of renal angiomyolipoma with hemorrhage should be based on clinic and imaging.CT and ultrasound have better diagnosis effect.Expectant treatment,selective embolization of renal artery,tumor excision and heminephrectomy are main methods.Nephrectomy can also be adopted when there is serious hemorrhage.