BACKGROUND:Bio-Gide col agen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide col agen membranes is rarely reported. OBJECTIVE:To observe the effect of Bio-Gide col agen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide col agen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by cellCounting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were col ected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days. RESULTS AND CONCLUSION:The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days (P<0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P<0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide col agen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.

BACKGROUND:Bioactive glass has good biocompatibility, which can promote the remineralization effects of demineralized enamel and enhance the resistance of enamel to erosion. OBJECTIVE:To evaluate the effect of bioactive glass toothpaste versus fluoride toothpaste on brushing abrasion of enamel after acid etching. METHODS:Fifty-six enamel specimens were prepared from bovine incisors, and were embedded in acrylic resin with exposed buccal surface. The exposed area was 6 mm×6 mm and two amalgam fil ings were placed into the enamel and polished. Al specimens were divided into seven groups with eight specimens each. In six groups, specimens were brushed with ordinary toothpaste, fluoride toothpaste, bioactive glass toothpaste immediately and 30 minutes after being eroded by the Sprite. The last one group was brushed with ordinary toothpaste without acid etching as control. The treatment course was cycled 60 times. The surface abrasion loss of the specimens between two amalgam fil ings compared with respective amalgam reference surfaces was final y observed and calculated under laser scanning confocal microscope. RESULTS AND CONCLUSION:The abrasion loss amount of teeth with no acid etching was significantly lower than that with acid etching (P<0.05). The abrasion loss amount of teeth brushed with bioactive glass toothpaste was less than that with fluoride toothpaste at the same brushing time (P<0.05). For the same toothpaste, the abrasion loss amount of teeth brushed 30 minutes after eroded was less than that bushed instantly after eroded (P<0.05). Use of bioactive glass toothpaste can effectively reduce enamel loss caused by acid etching and brushing. The loss amount can also be reduced by brushing teeth 30 minutes after acid etching.

BACKGROUND:Studies have found that the glass fiber splint which is embedded into the groove on the lingual surface of the affected tooth plus fluid resin fixation between the gap of mobile teeth can achieve more satisfactory clinical effects on the stabilization of loose front teeth.
OBJECTIVE:To observe the clinical effects of glass fiber splints prepared using two tooth preparation methods on splinting loose front teeth and their debonding rate through a 5-year fol ow-up visit.
METHODS:Ninety-four chronic periodontal disease patients, who had received periodontal treatment, were enrol ed in this study for their loose front teeth of degree II-III. Al the enrol ed patients were good candidates for periodontal splinting strictly in accordance with the indications for periodontal splinting. According to whether the fluid resin was used to seal the gap between the mobile teeth, the 96 patients were divided into two groups:patients undergoing glass fiber splint+fluid resin between the mobile teeth served as experimental group, and those only undergoing glass fiber splint as control group. Al the patients were fol owed by regular clinical referral or telephone fol ow-up for 5 year, to evaluate the fixed effects.
RESULTS AND CONCLUSION:During the 5 years of regular clinical referral or telephone fol ow-up, seven of the 76 cases and 12 of the 18 cases were found in the experimental and control groups, respectively, to have debonding between the abutment and the splint. Compared with the control group, the probing depth and attachment loss of affected teeth were improved significantly (P<0.05), and the percentage of alveolar bone height accounting for the root length increased significantly in the experimental group (P<0.05) after 5 years of fol ow-up. Patients in the experimental group felt comfortable without foreign body sensation. The results confirmed that the glass fiber splinting plus fluid resin fixation between the gap of mobile teeth was a better treatment for loose front teeth during the 5-year fol ow-up.

It is nonlinear relationship between breath sounds and respiratory flow rate, thus breath sounds might be a new solution for respiratory monitoring. The envelope of the breath sounds was created firstly. And the linear relationship between the logarithm of envelope and the respiratory flow rate was proved. Then model parameters were derived from every subject using regression analysis. Finally, using these parameters estimated respiratory flow rate was achieved by the logarithm of envelope with 14.9% error. Therefore, respiratory flow rate estimation and respiration monitoring based on breath sounds are feasible.
Humans ; Monitoring, Physiologic ; Respiratory Sounds

[Objective]To evaluate the operative technique and the short-term clinical results of total hip arthroplasty(THA) for developmental dysplasia of the hip(DDH) in adults. [Method]Sixty-one patients(77 hips) with DDH who received THR without cement between December 1997 and October 2006 were followed up.In this group,53 cases were female,and 8 cases were male.The average age was 31.4 years(range,17~56 years).According to the classification of Hartofilakidis,fifteen cases were type I(eighteen hips);twenty-three cases,type Il(thirty hips);and twenty-three cases,type III(twenty-nine hips).The average Harris Hip Score was 40 points(range,16~61).All of patients were exposed through the.posterolateral approach.The threaded cup with coating were put in or near the level of the true acetabulum in all of cases.Full coating stems were used in femoral side,shortening osteotomy below lesser trochanter of femur were performed in twelve cases. [Result]The mean duration of follow-up was 49 months(range,12~118 months),the average Harris Hip Score increased from 40(range,16~61) preoperatively to 91(range,82~95) at the time of the latest follow-up.All the hips acquired union of osteotomy and bone ingrowth.None of the patients had radiographic evidence of aseptic loosening of prosthesis. [Conclusion]Uncemented total hip arthroplasty for the developmental dislocation of the hip in adults can get better results significantly,although it presents greater technical difficulty.

Objective:To explore the influence factors and the characters of infant-mother attachment in Guangdong.Methods:“strange situation"essay was used to assess the characters of infant-mother attachment in 75 infants of 8 to 14 months.An self-formulated questionnaire of infants'general status and family related factors were filled by their mothers.Results:(1)Among 75 infants,65.3% were secure-type,34.6% were insecure-type which included 17.3% of indifferent-type, 13.3% of importunate-type,and 4.0% of disorganized-type;(2)The influence factors were age,approachability,relations between infants and fosterers, and the attitude consistency among family members towards infants.Conclusion:Of this investigation, most infants were secure-type.There were some influence factors involved in the characters of infant-mother attachment.

BACKGROUND:At present, it remains controversial whether open reduction plate fixation is needed for distal radius fracture in the elderly to restore anatomic reduction of imaging.
OBJECTIVE:To systematical y evaluate the curative effects of operative and nonoperative treatments for distal radius fractures in the elderly.
METHODS:We retrieved PubMed, Springer and Wanfang database using computer. Relevant English and Chinese journals of orthopedics were retrieved by hand. Al literatures on curative effects of operative and nonoperative treatments for distal radius fractures in the elderly were col ected. RevMan 5.0 software provided by Cochrane was used to statistical y analyze the data.
RESULTS AND CONCLUSION:Six literatures were included, including two of randomized control ed study and four of retrospective cohort study. Meta-analysis results displayed that there were no significant differences in both groups in range of motion of wrist joint extension [mean difference (MD)=-0.87, 95%confidence interval (CI) (-3.31, 1.58), P=0.49], range of motion of wrist joint flexion [MD=-2.79, 95%CI(-6.47, 0.88), P=0.14], range of motion of wrist pronation [MD=-0.08, 95%CI(-1.49, 1.64), P=0.92], range of motion of supination [MD=-0.7, 95%CI(-3.52, 2.12), P=0.63] and the occurrence of complication [MD=1.35, 95%CI(0.71, 2.56), P=0.36]. The length of radius in the surgery group was similar to that of normal [MD=2.46, 95%CI(1.78, 3.15), P<0.01]. Ulnar inclination [MD=3.73, 95%CI(2.97, 4.48), P<0.000 01] and palmar tilt angle [MD=6.81, 95%CI(3.72, 9.90), P<0. 000 1] in the surgery group were close to that of normal. There are no significant differences in extension, flexion, pronation and supination activities of wrist and posttreatment complication between two groups. Operative treatment result is superior to that of nonoperative treatment in anatomic reduction.

Objective To explore the effect of improved local nerve block anesthesia in correction of nasal septum surgery.Methods One hundred and fifty-one cases of patients with simply received correction of nasal septum surgery were divided into two groups,72 cases with normal local nerve block (normal group),receiving sphenopalatine and anterior ethmoidal nerve block anesthesia; 79 cases with the improved local nerve block (improved group),receiving sphenopalatine,anterior ethmoidal and incisive canal nerve block anesthesia.Two groups of anesthesia success and anesthesia effect were compared.Results The anesthesia success rates of improved group and normal group who were performed correction of nasal septum surgery without removal of the nasal bottom ridge were 100.0％(41/41) and 97.3％ (36/37),there was no significant difference (P＞ 0.05).But for 73 cases who were performed correction of nasal septum surgery with removal of the nasal bottom ridge,the anesthesia success rate in improved group was higher than that in normal group [92.1％(35/38) vs.45.7％(16/35)],there was significant difference (x2 =18.623,P＜ 0.05).Conclusions Based on the distribution characteristics of sensory nerves of the nasal cavity,and combined with endoscopic techniques,the conventional sphenopalatine and anterior ethmoidal nerve block anesthesia is suitable for nasal septum surgery without removal of the nasal bottom ridge and the advanced sphenopalatine,anterior ethmoidal and incisive canal nerve block anesthesia is the first choice for nasal septum surgery with removal of the nasal bottom ridge.

Objective To evaluate the effect of open reduction and multi-dimensional several pin fixation of Gartland type Ⅲ supracondylar fractures with late callus formation in children.Methods The study involved 37 children of Gartland type m supracondylar fractures with late callus formation undergone posteromedial elbow incision,open reduction via bilateral triceps sparing approach,several K-wire internal fixation and early functional rehabilitation from May 2009 to May 2011.There were 23 males and 14 females at 3-13 years of age (mean,7 years).Postoperative results were evaluated according to Flynn standard.Mean 9.5 months of follow-up was made.Results The results were excellent in 28 cases,good in five,fair in two and poor in two.No serious complications occurred after operation.Conclusion Open reduction and multi-dimensional several K-wire fixation is an effective treatment for Gartland type Ⅲ supracondylar fractures with late callus formation in children and hence provides a choice for clinical use.