1.Clinical Observation of Aciclovir and A-Polyresistin Theraphy on Children with Recurrent Oral-Ulceration
jun, ZHANG ; ping, ZHANG ; chuan-sheng, WANG
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To observe the effect of immunity condition and clinical manifestations of children with recurrent oral-ulcer(ROU)treated with aciclovir(ACV) and a-polyresistin.Methods Forty-eight patients with ROU were randomly divided into whole treated group(25 cases)and partial treatment group(23 cases).The changes of their peripheral blood lymphocyte subgroups,before and after treatment,were measured and contrasted to analyze the relationship with curative effect.Results The ratios of CD3,CD4,and CD4/CD8,after the treatment,increased obviously,while CD8 lowered obviously in treatment group(P0.05).The total clinical efficacy rate was 47.82%,moreover the patients were recurrent after 1 year.Conclusions ACV and a-polyresistin,through antivirus and regulating the immunity function get the 96% total clinical efficacy rate,and that controle the recurrent rate.It is superior to the traditional method.
3.Arthroscopic medial patellofemoral ligament reconstruction combined with lateral retinacular release for recurrent patellar dislocation.
Qi-chuan ZHANG ; Su-fang WANG ; Xin-sheng FU
China Journal of Orthopaedics and Traumatology 2015;28(7):599-602
OBJECTIVETo evaluate the clinical results of the medial patellofemoral ligament (MPFL) reconstruction combined with the lateral retinacular release for the treatment of recurrent patellar dislocation.
METHODSFrom March 2011 to June 2013, 15 patients with recurrent patellar dislocation underwent arthroscopic MPFL reconstruction combined with the lateral retinacular release. The graft was autogenous semitendinosus and semimembranosus tendon. There were 5 males and 10 females with an average age of 19.4 years old (ranged,14 to 32 years old). The patients suffered recurrent patellar dislocation at least twice preoperatively. Preoperative conventional X-ray, CT, and MR examination were used to analyze the causes of the patellofemoral joint and MPFL injury. Preoperative Lysholm score was 69.85 ± 11.52. During operation, the arthroscopic examination was performed to evaluate the patellofemoral alignment and patellar tracking.
RESULTSAll the patients were followed up for an average of 27.6 months (ranged,12 to 36 months) with no recurrent dislocation and sub-dislocation. All the patients showed negative apprehension test at straight and 30 ° flexions of knee. The range of motion of knee returned to normal level at 12 months after operation. There were no patients with subjective discomfort of knee. Postoperative Lysholm score was improved to 92.60 ± 5.75.
CONCLUSIONThe technique of arthroscopic MPFL reconstruction combined with the lateral retinacular release is an effective surgical procedure for the treatment of recurrent patellar dislocation, which can relieve the symptom of knee and improve the patella stability and knee function.
Adolescent ; Adult ; Arthroscopy ; Female ; Humans ; Knee Joint ; surgery ; Male ; Patellar Dislocation ; physiopathology ; surgery ; Patellar Ligament ; physiopathology ; surgery ; Patellofemoral Joint ; physiopathology ; surgery ; Range of Motion, Articular ; Treatment Outcome ; Young Adult
4.Animal Experimental Study of the National EVAL Embolic Mixture
Jiangtao WANG ; Zuoqin LIU ; Chuan LU ; Xizhong SHENG
Journal of Practical Radiology 2000;0(02):-
Objective To study the technical feasibility and embolic effect of embolism with the national ethylene-vinyl alcohol copolymer(EVAL)embolic mixture in an animal model.Methods 6 swines,25~30 kg,no matter male or female were used in this study.Superselective catheterization of ascending pharyngeal artery or rete mirable(RMB) was performed under general anaesthesia,then EVAL embolic mixture was injected to make the vascular embolism until satisfaction under fluoroscopy.The swines after embolism were followed-up 1 day,1 week,4 weeks,8 weeks,12 weeks,24 weeks and DSA,histopathological examination were respectively done.Results The embolic programes were successful in all animals,but one because of over reflux,and the embolic blood vessels could not be showed on angiography.In gross pathology,black embolic EVAL inside the vessels could be seen,which were firm and easily dissected.In histopathology,in the acute phase,all of vessel walls were destroyed in different degree,there were many neutrophils and eosinophils around the EVAL embolic mixtures and out of the vessels,and a lot of red cells could be seen,in subacute and chronic phase,mild to moderate mixed inflammatory infiltrates with histoleucocytes and large foreign-body giant cells were seen in occluded vessels.Conclusion The national EVAL embolic mixture is a suitable and easy control vascular embolic material.
5.Effects of quetiapine and risperidone in the treatment of behavior and psychiatric symptom of dementia
Tao LOU ; Ning LI ; Chuan-Sheng WANG ; Zhi-Feng ZHANG ; Jian-Ying WANG ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(09):-
Objective To investigate the effects of quetiapine and risperidone in the treatment of behavior and psychiatric symptom of dementia(BPSD).Methods 56 cases of Alzheimer's disease and vascular dementia pa- tients were all met for the classification and diagnostic criteria for mental illness in China(CCMD-3)and randomly divided into two groups(27 in quetiapine group,29 in risperidone group),experimental dosage were 0.05~0.3g/d and 1~2mg/d,respectively.The treatment lasted for 8 weeks.Results The total efficiency of quetiapine was 66.67% and that of risperidone was 65.5%,and the statistical difference was significant.As for the adverse effects, there was no significant difference,but incidence of EPS in quetiapine group was less than that of risperidone group. Conclusion Quetiapine can be widely administrated in clinic application as the high efficacy of control BPSD with low minor adverse effect.
7.Package and identification of replication deficient recombinant adenovirus expression vector of channelrhodopsin-2
Jun-ping, YAO ; Wen-sheng, HOU ; Hao, WANG ; Chuan-huang, WENG ; Zheng-qin, YIN
Chinese Journal of Experimental Ophthalmology 2012;30(8):681-685
Background Channelrhodopsin-2 (ChR2)is a cation channel isolated from the eyespot of Chlamydomonas algae and has been used to control neuron activity.The light stimulation is a more precise fashion whether space or time than that of electrical,magnetic and ultrasound stimulation. Objective This study was to construct a replication deficient recombinant adenovirus cxpression vector of ChR2 and to determine its function.Methods Human embryo kidney 293 (HEK293) cell line was cultured and passaged in DF12 medium containing 10% fetal bovine serum(FBS).The ChR2 gene was cloned at the downstream of cytomegalovirus(CMV)promoter of the adenoviral shuttle plasmid pSB291 in sense direction,and the resultant recombinant plasmid pSB291-hChR2- GFP was transfected into HEK293 cell together with plasmid pBHG lox ( deltaE1,3 ) containing adenoviral genome,then small amounts replication deficient recombinant adenovirus expression vector of ChR2 (Ad-ChR2) was obtained.Through amplification gradient centrifugation and dialysis,pure Ad-ChR2 was obtained.Visual cortex cells derived from 4 1-day-old clean Long Evans rats were primary cultured with serum-free culture media and infected by AdChR2.When expressing green fluorescencc,those cells received the stimulated of blue light with 460 nm.Patch clamp technique was applied to record an action potential. Results After purification and concentration,the titer of AdhCHR2 reached 7.9×1010 PFU/ml.Twenty-four hours after transfect of Ad-ChR2,HEK293 cell membrane showed the green fluorescence for the recombinant plasmid with green fluorescence protein under the inversed fluorescence microscope.The HEK293 cells change their shape from flat to round 13 days after transfected.The primary cultured visual cortex cells exhibited the green fluorescence 3-5 days after infected by Ad-ChR2.The action potentials evoked by blue light stimulation were recorded with patch clamp on those cells expressing green fluorescence. Conclusions Ad-ChR2 expressing vector is constructed successfully in this study.It is verified that Ad-ChR2 expressing vector can infect visual cortex cells with visual function.This result is very important for visual plasticity study.
8.Reconstruction of rabbit knee joint cartilage defect using tissues engineering method
Hongfeng JIANG ; Wei WEI ; Yun-Chuan XIE ; Rong-Rui LI ; Rao-Sheng ZHAI ; Ri-Guang WANG ;
Chinese Journal of Microsurgery 2000;0(03):-
Objective To estimate curative effect of reconstruction of rabbit knee joint cartilage defect with the homogeneitic tissue engineered cartilages.Methods The chondrocytes were isolated and collected from articular cartilages of eight New Zealand white rabbits.The tissue engineered cartilages after culturing chondrocytes and atelocollogen for two days.Cartilage defects were created in both keen joint of twenty-six rab- bits.Complexes of chodrocytes and atelocollagen was grafted into the defect of left knee joint at once as experi- mental group,and no implantation were served as control.General and histological examination were respec- tively performed in both group at four weeks and eight weeks after surgery.Results After implantation,the defects were filled with cartilaginous tissue in experiment group,while there were only tissue in control group. Histologically,defective areas were filled with chondrocytes in experiment group,but only fibroblast in control group.Conclusion The implantation of the tissue engineered cartilages contenting with chondrocytes and atelocollogen can effectively improve reconstruction of rabbit knee joint.
9.Free vascularized fibular associated with iliac graft to treat old femoral neck fracture
Kunzheng WANG ; Chun-Sheng WANG ; Xiao-Qian DANG ; Jin-Hui SONG ; Hong-Wei YAN ; Chuan-Yi BO ;
Chinese Journal of Microsurgery 2000;0(03):-
Objective To evaluate the clinical results of free vascularized fibular associated with iliac graft in treatment of old femoral neck fracture.Methods From 1994 to 1997,76 cases of old femoral neck fracture were treated with free vascularized fibular associated with iliac graft,they were followed up for observ- ing fracture healing,hip function and femoral head necrosis.Results Sixty-eight of these 76 cases were fol- lowed up with detail data with the main term as 10.5 years.All fractures healed within 4 to 6 months.63 cases got a almost normal hip function with the Harris score was 87.5,in these cases,the structure of femoral head were normal and the grafted fibular and iliac bone healed with the femoral.4 cases got to femoral head necrosis and 1 experienced hip dislocation.Conclusion Free vascularixed fibular associated with iliac graft is a good method to treat these cases with old femoral neck fracture who want reserve their femoral head.
10.Antiviral effect of human CTLs activated by HBsAg stimulated dendritic cells in vitro.
Li-he XING ; Fu-sheng WANG ; Chuan-lin ZHU
Chinese Journal of Experimental and Clinical Virology 2003;17(4):365-368
OBJECTIVETo investigate the ability of human dendritic cells (DCs) inducing specific T lymphocyte response and inhibit the expression of HBeAg and HBsAg in 2.2.15 cell culture supernatant.
METHODSDCs were prepared from peripheral blood mononuclear cells induced with granulocyte macrophage colony-stimulating factor(GM-CSF) and interleukin 4. DCs was impulsed with pure HBsAg before DCs maturation and cocultured with self-blood T lymphocyte, while DCs without pure HBsAg stimulated group, T lymphocyte group and only T lymphocyte group were prepared as control group. The culture supernatant of 2.2.15 cell with stimulated T lymphocytes was collected on day 1, day 3, day 5 and day 7, respectively. The expressed levels of HBeAg and HBsAg were detected by ELISA method.
RESULTSDCs after antigen stimulation had a strong ability to present antigen and induce immune activation, DCs after loading with antigen in normal control and chronic hepatitis patients group had stronger stimulative ability for T lymphocytes proliferation than that of DCs without loading with antigen and only T lymphocyte group(P less than 0.01). The stimulating ability of DCs had a positive correlation to the dosage of loaded antigen; CTLs produced as a result of DCs stimulation had a specific inhibitive effect on the expression of HBeAg in 2.2.15 cell supernatant,but not on the expression of HbsAg.
CONCLUSIONHuman dendritic cells stimulated with HBsAg in vitro can efficiently present antigen and stimulate the production of specific CTLs to HBV, which can efficiently inhibit the expression of HBeAg in 2.2.15 cell supernatant- DC vaccine may become an antiviral therapy strategy for chronic hepatitis B virus infected patients in future.
Adolescent ; Adult ; Antigen Presentation ; Cells, Cultured ; Child ; Dendritic Cells ; drug effects ; immunology ; Female ; Hepatitis B Surface Antigens ; pharmacology ; Hepatitis B, Chronic ; immunology ; Humans ; Lymphocyte Activation ; Male ; Middle Aged