1.Electroacupuncture regulates pain and its effect on IL-17 and IL-17R expression of spinal cord in New Zealand rabbit with osteoarthritis
Chongqing Medicine 2017;46(17):2326-2329
Objective To investigate whether electroacupuncture(EA) regulates the pain in New Zealand rabbits with osteo arthritis by inhibiting the IL-17 and IL-17R expression in spinal cord.Methods New Zealand rabbits were randomly divided into the sham operation group,inflammation model group,sham operation+ IL-17 group,inflammation+ IL-17 group,inflammation + EA group and inflammation +sham EA group,8 cases in each group.The rabbit osteoarthritis model was established by using the papaya protease,IL-17 and IL-17 antiserum was injected into the spinal canal,The EA group selected the EA at Zusanli point(30 min,2 Hz continuous wave,1~ 2 mA),the sham EA group adopted the non-acupoint stimulation.Then the pain threshold of rabbits was detected.The mRNA expression of IL-17 and IL-17R in rabbit spinal cord tissue was detected by RT-qPCR and the protein expression was detected by Western-Bolt.Results The rabbit pain threshold was significantly decreased after constructing the New Zealand osteoarthritis model(P<0.05),and the spinal canal injection of IL-17 could significantly decrease the pain threshold of sham operation New Zealand rabbit(P<0.05),while the spinal canal injection of IL-17 antiserum could significantly increase the pain threshold of osteoarthritis model rabbit.Selecting EA at Zusanli point could significantly enhance the rabbit pain threshold (P<0.05).The mRNA and protein expression of IL-17 and IL-17R of the osteoarthritis model group were significantly increased (P<0.05),while the expressions were significantly decreased after EA stimulation(P<0.05).Conclusion The pain occurrence of New Zealand rabbit osteoarthritis may involve the IL-17 abnormal expression in spinal cord.EA may regulate the osteoarthritis pain by inhibiting the expression of IL-17 and IL-17R in spinal cord.
2.Analysis of Problems with cultivating medical morality for medical students and relevant reasons
Chinese Medical Ethics 2016;29(2):202-204
The research carried out a questionnaire regarding education of medical morality at four medical colleges in Fujian province, it found some problems existed, for example, the education of medical morality is ful-filled without knowing medical classical works, being short of systematicness, having no reviewing and encouraging mechanism. The article discusses the cause of the problem. ,with the reality of theInternet +, the characteris-tics of the medical students′education pattern and the characteristics of the medical ethics education.
3.Effects of angiotensin Ⅳ on growth of cultured neonatal rat cardiomyocytes
Chinese Journal of Pathophysiology 1989;0(05):-
AIM: To study the effects of angiotensin Ⅳ(Ang Ⅳ) on growth of cultured neonatal rat cardiomyocytes.METHODS: Cardiomyocytes isolated from neonatal SD rat were cultured in vitro and divided into groups: control DMEM,10~(-6) mol/L Ang Ⅳ, 10~(-5) mol/L Ang Ⅳ.The effects of Ang Ⅳ were assayed as follows: protein synthesis was determined by modified Bradford method,and cell cycle by flow cytometry.RESULTS: Ang Ⅳ promoted protein synthesis(P
4.Protective effects of total extract of astragalus(TEA) onprimary rats hepatocytes injured by CCl4 or H2O2
Yan YANG ; Min-Zhu CHEN ;
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To study the protective effect of total extract of astragalus(TEA) on primary rat hepatocytes injured by CCl4 or H2O2 and its mechanism.Methods The primary hepatocytes were isolated by Ⅳ collaganase and injured by CCl4 or H2O2.The contents of malondialdehyde(MDA) and glutathion(GSH),glutathion peroxidase(GSHpx) activity of hepatocytes and the AST and /or ALT level in cultural supernatant were determined by general methods. Results (1) The elevation of MDA content of hepatocytes and AST level in supernatant of cultural hepatocytes,and the loss of GSH content and GSHpx activity induced by CCl4 were restored remarkably by TEA(5~80 mg?L-1);(2)The elevation of ALT level in the supernatant of hepatocytes and MDA content of hepatocytes, and the loss of GSH content and GSHpx activity induced by H2O2 were improved significantly by TEA(5~80 mg?L-1) treatment.Conclusion The results suggest that TEA possess direct protective action on primary hepatocyte in vitro injured by CCl4 or H2O2. These might be associated with its anti-oxidative activity.
5.Analysis on the System of Performance Audit Indicators under New Rural Cooperative Medical System
Yufan CHEN ; Peng CHEN ; Min YANG
Chinese Health Economics 2014;(5):89-92
Objective: To propose a reasonable and feasible system of performance audit indicators for New Rural Cooperative Medical System(NCMS). Methods:Using the balanced score card as amended to perform the evaluation. Results:From 5 dimensions, 37 indicators were used to perform comprehensive evaluation for the NCMS. Conclusion: The indicator system is suit for the current situation, as well as audit evidence through questionnaires to obtain the required data of the system;it provides a better evaluation of the NCMS performance.
6.Effect of polyunsaturated fatty acid on the expression of Huntingtin associated protein 1 in hippocampus of rat model with Alzheimer' s disease
Rong YANG ; Youhua YANG ; Dan HUANG ; Min YANG ; Xiaoqing CHEN
Chinese Journal of Behavioral Medicine and Brain Science 2010;19(10):882-884
Objective To study the effect of docosahexaenoic acid ( DHA ) and eicosapentaenoic acid (EPA) on cognition impairment and huntingtin associated protein 1 ( HAP1 ) expression in hippocampus of rat model with Alzheimer' s disease(AD). Methods Forty healthy Sprague-Dawley rats were randomly divided into control group, AD group, DHA group and EPA group on average. Alcl3 was injected intraperitoneally and D-galac tose was injected into subcutaneous to establish the model of rat with AD. ABC method of immunohistochemistry was used to observe the expression level of HAP1 in the hippocampus. Morris water maze was used to study the spatial learning and memory in rats. Results Compared with control group,the HAP1-positive neurons of hippo campus decreased and poorer performance in Morris water maze test was observed in AD group. Compared with AD group, DHA and EPA treatments significantly caused the decreases in escape latency and searching distance in the Morris water maze test. The number of HAP1- positive cells in DG region of DHA group(43.57 ±6.14) increased obviously compared with AD group( 28.56 ± 4.23 ) (P < 0.05 =. Conclusion The immunoreactivities of HAP1 decrease in hippocampus of AD rat. The applications of DHA and EPA in AD rats significantly improve the ability of learning and memory;and the mechanisms may be related to the decrease of HAP1 expressions in hippocampus.
7.An analysis on influencing factors of elders' medical consumption behavior under the back-ground of pension combined with medical service:A case study of Nanjing
Chinese Journal of Health Policy 2017;10(1):52-57
Objective: Under the background of pension combined with medical service, for the elders to reasonably, effectively and fully utilize the medical resources, it is of vital significance to establish good medical con-sumptive behavior and consumption intention, to promote healthy old age and improve elders' health and happiness to help them cultivate a fine habit in their practical life. Methods: Based on the micro-survey data of investigation in Nanjing, this paper systematically analyzed the factors influencing elders' medical consumption behavior by using the theory of planned behavior and structural equation model. Results:Different factors were classified into behavioral at-titudes, subjective norms and perceived behavior control, and significant variables were found out through behavior model as well as though measuring their relative effects. Conclusion: There is no significant casual relationship be-tween the perceived behavior control and medical consumption behavioral intention. The elders' medical consumption behavior intention is affected by various social network relationships. The belief strength, normative belief, behavioral attitudes and subject norms can affect elders' medical consumption behavior.
8.EFFECTS OF 70% HEPATECTOMY ON THE PROLIFERATION OF RAT FETAL HEPATOCYTES AFTER INTRASPLENICAL TRANSPLANTATION
Mingzhi YANG ; Jun MIN ; Jishen CHEN
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
To study the effects of 70%Hepatectomy on the proliferation of rat fetal hepatocytes after intrasplenical transplantation, fetal hepatocytes isolated from 3 week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of hepatocytes in the remanent liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by Image Analysis System 7 and 30 days post transplantation respectively. Compared with the control group, the proportions of S state cells in the remanent liver were obviously increased ( P
9.Expression of the CD62,CD63 in Peripheral Blood to Predict Clinical Outcome in Women with Preeclampsia
Shujun YANG ; Yunfei LI ; Min CHEN
Journal of Chinese Physician 2001;0(05):-
Objective To evaluate the expression of adhesion molecules CD62,CD63 and abnormal functional status of platelets in women with preeclmpsia and their relation to progression.Methods The expression of surface antigens CD62,CD63 in peripheral blood and functional status of platelets were measured by flow cytometry in 68 women with preeclmpsia,30 non-pregnant normal women and 52 normal pregnancy women as the controls.Results There was no significant difference among defferent stages in normal pregnant women,but the CD62,CD63 positive platelets were (7 41?1 98)%,(12 99?2 37)% and (36 52?8 92)% respectively for mild,moderate and severe preeclmpsia,it double high (P
10.Relationship between parathyroid hormone changes in patients with chronic renal failure and cerebrovascular events
Yaping JIANG ; Hailong CHEN ; Min YANG
Chinese Journal of Primary Medicine and Pharmacy 2010;17(17):2332-2333
Objective To investigate the relationship of iPTH levels, calcium and phosphorus metabolism with cardiovascular event status in the chronic renal failure (CRF) patients. Methods This article retrospectivly studied the clinical and laboratory data of 87 CRF patients. 66 patients of A group didnt occur cardiovascular events,and 21 cases of B group occurred cardiovascular and cerebrovascular events. This article analyzed iPTH levels,serum calcium, phosphorus, calcium and phosphorus product and therelationship between cardiovascular and cerebrovascular events between the two groups. Results Decreased iPTH,increased serum C reactive protein and increased lipoprotein(a) had relationship with cardiovascular and cerebrovascular events in patients with chronic renal failure,but calcium,phosphorus,calcium and phosphorus product has nothing to do with the cardiovascular and cerebrovascular events. Cardiovascular and cerebrovascular events increased significantly in dialysis patients than in non - dialysis patients. Conclusiou Chronic renal failure patients on dialysis levels of serum iPTH, CRP level, LP(a) level and dialysis could lead to cardiovascular and cerebrovascular events.