Objective To study the the effectiveness of Entecavir on serum transforming growth factor-β1 (TGF-β1) in patients with decompensated cirrhosis .Methods Eighty patients with decompensated cirrhosis were randomly divided into two groups ,the control group(n=40 cases) and the observation group (n=40 cases).The pa-tients in the control group were treated through the conventional treatmeat ,while the patients in the observation group were treated through the conventional treatment plus entecavir .They were treated for 24 weeks.Liver function,HBV DNA and serum TGF-β1 were detected.Results ALT,Tbil and ALB were improved after treatment in both groups (t=6.724,13.306,3.064,4.172,2.924,3.172,all P<0.05).They were more significantly improved in the obser-vation group than that in the control group (t=2.469,2.160,3.064,all P<0.05).HBV-DNA negative rate in the observation group was higher these in the control group (χ2 =4.82,P<0.05).Serum TGF-β1 in both groups were decreased after treatment(t=6.842,8.062,all P<0.01).There was a significant difference between two groups (t=3.178,P<0.05).Conclusion Entecavir can decrease serum TGF-β1 in patients with decompensated cirrhosis .

Objective To evaluate the expressions and roles of survivin (SVV), bcl-2 and proliferating cell nuclear antigen(PCNA) in cutaneous squamous cell carcinoma (SCC). Methods The expressions of SVV, bcl-2 and PCNA were investigated by SP immunohistochemistry technique in 82 cases of SCC and 18 normal skin samples. Results There were 79.3% of SCC patients with the expression of SVV (P

OBJECTIVE:To further exploit the active components from Loquat leaf and its gruffs.METHODS:The chief components in loquat leaf and its gruffs were extracted by ultrasound extraction and Soxhlet extraction,respectively,the sepa?ration of ursolic acid and other constituents was performed by HPLC,and the relative amounts of which were determined and compared.RESULTS:Soxhlet extraction is superior to ultrasound extraction in the extraction of loquat leaf and8components in gruffs.Under the same extraction method,the content of ursolic acid in loquat leaves is equivalent to that of gruffs.Besides,the contents of6components in gruffs were above those in loquat leaves.CONCLUSION:The essential component-triterpenic acid in loquat leaves suffered no loss after extraction with water,the active components in gruffs can be further exploited.

The hypoxic pulmonary vasoconstriction (HPV) in Yunan monkey has been observed. The results showed that acute alveolar hypoxia caused significant pulmonary hypertension, associated w~th art increase in pulmonary vascular resistance. The maximal response appeared 3 min after onset of hypoxia. Monkey may be proposed as moderate responder in HPV. The HPV in monkey was blocked by lipoxygenase inhibitor diethylcarbamazine and enhanced by cyclooxy genase blocker...indomethacin. It is suggested that lipoxygenase metabolites leukotrienes probably play the role of a mediator in HPV of monkey, whereas cyclooxygenase metabolites prostaglan dins act as medulator.

Objective To elucidate the effect of early enteral nutrition support(ENS) for the patients with severe acute pancreatitis(SAP).Methods Fifty patients with SAP were at random divided into two groups: enteral nutrition group(EN group, 25 cases), parenteral nutrition group(PN group, 25cases). PN group was given total parenteral nutrition(TPN),125 kJ/(kg?d) of energy, nitrogen intake:0.20g/(kg?d). In EN group, the naso intestinal tube was placed posterior to ligament Treitz. Since the 1st day nutrilion was infused.Results On the first?4th day, the serum RBP?PA levels were obviously decreased (P

Objective To investigate surgical treatment methods of the patients with severe acute pancreatitis complicated pancreatit necrosis (IPN) and pancreatic abscess (PA). Methods The operation of removing pancreatic abscess,infected necrosis tissues in the pancreas and peripancreatic tissues were performed. Additional, abdominal cavity infusion and drainage also were performed.Results 12 patients with IPN and 3 patients with PA were cured. Four times of operation was performed in a paitents with IPN and his corpus vertebras are infected and destructed. Another patients was relapsed after 1 year and was again performed 2 times of operation. The patient is in the stage of recovery.Conclusions IPN and PA are severe complications of severe acute pancreatitis. If image examination were showed that hydrops could not be extincted in the pancreas and peripancreas, operative therapy should be performed.

Objective To explore the clinical and molecular-genetic features and prognosis of mitochondrial encephalopathy in children. Methods A total of 11 patients diagnosed with mitochondrial encephalopathy were collected from 2008 to 2013 in Chinese PLA General Hospital, Department of Pediatrics. The clinical manifestations, laboratory tests, muscle pathology and mitochondrial gene sequencing in patients were analyzed and followed-up. Results The onset age of the patients ranged from 6 months to 12 years with the duration of disease from 2 months to 3 years. Of the 11 patients, 6 cases were diagnosed with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS), 5 cases with mitochondrial disorder induced by mitochondrial respiratory chain enzyme deifciency. Main manifestations included convulsions, vomiting, headache, mental retardation, paralysis, etc. 10 patients had elevated lactate, and 7 patients had elevated pyruvate;6 cases showed increased slow wave in EEG background;Cranial MRI showed the damage involved in bilateral basal ganglia in 2 cases, temporal and occipital lobes in 3 cases, , multiple lesions in 2 cases, frontal and parietal occipital lobes in one patient and hypothalamus in one patient;3 patients were underwent MRA examination, 2 cases were normal, the other showed less shunts in contralateral left artery in the brain;3 cases who underwent MRS showed lactate peak. 2 cases of children underwent skeletal muscle biopsy, and one case showed abnormal mitochondria accumulation. Mitochondrial respiratory chain complex (I~V) defects test showed complex IV defects in 2 cases, complex V defects in 1 case, and combined defects of complex I+III in 2 case. 5 cases were found with mitochondrial DNA mutations in different sites of white blood cells including T8993G, T8993C, A3243G and 11,777 mutations. Conclusions Clinical manifestation of mitochondrial encephalopathy in children is strongly heterogeneous. Laboratory tests, brain imaging, gene mutation and respiratory chain enzyme examination can help to make early diagnosis and treatment. These diseases have poor prognosis.

Objective To analyze the causes of poor compliance of elderly patients with diabetes mellitus , and put forward corresponding countermeasures to control blood glucose level and delay the occurrence of complications .Methods A total of 113 elderly patients with type 2 diabetes mellitus aged 60 years and over were recruited in endocrinology department of our hospital.The self-made questionnaire was used to investigate the compliance and the factors that affected the compliance.Results There were 48 patients (42.48%) with good compliance and 65 patients (57.52%) with poor compliance.Univariate analysis showed that the level of education, monthly income, drug types, understanding of diabetes-related knowledge and whether the elderly living alone affected compliance ( P<0.05 ) .Multivariate Logistic regression analysis showed that patients with low education level, poor monthly income, medication types and living alone were the main factors that influence the compliance of elderly patients with diabetes mellitus (P<0.05).And the level of education, monthly income level and the compliance of diabetic medication was positively correlated, medication types and living alone and diabetes compliance were negatively correlated (P<0.05).Conclusion The low level of education, poor monthly income, medication types and living alone are the main factors that affect the compliance of elderly patients with diabetes.The social and family support can effectively improve the compliance of elder patients with diabetes.

Objective To investigate the effect of Yangyinqingfei granula on immune function in the treatment of mycoplasma pneumonia complicated with Epstein-Barr virus of children.Methods 108 mycoplasma pneumonia complicated with Epstein-Barr virus of children from our hospital were selected and randomly divided into two groups.The control group of 54 cases were treated by simple western medicine treatment and the experimental group of 54 cases were treated on the basis of the control group with Yangyinqingfei granula.The improvement of clinical symptoms, inflammation indexes and the levels of T lymphocyte subsets, clinical efficacy and complications were compared.Results The clinical efficacy in experimental group was 90.74%,which was significant higher than that in control group 75.93%(P <0.05).Compared with the control group,the remission duration of fever, cough and rales,the disappearance time of lung X-ray,the length of stay were shorter(P<0.05), the CRP,IL-6,TNF-αand IL-8 levels were lower ( P <0.05 ) , the CD3 +, CD4 +, CD4 +/CD8 + and NK levels were higher, the CD8 +level was lower ( P <0.05 ) . Conclusion Yangyinqingfei granula could reduce the inflammatory factors and improve immune function in the treatment of mycoplasma pneumonia complicated with Epstein Barr virus infection pneumonia in children.

OBJECTIVE To compare the detection rates of Ureaplasma urealyticum(Uu),by 4 kinds rits and find out the best one.METHODS Using four kinds of mycoplasma detection kits,namely are French mycoplasmaIST2,Zhuhai Livzon′s ICS,Dark horse′s IES and Shanghai Its Prachanda,under the same conditions,the mycoplasma of the Clinical specimens for 260 cases were tested.RESULTS The detection rate of mycoplasma(Uu) for 48 hours by four kinds of brands kits about mycoplasma liquid culture was as follows 45.4% for French MycoplasmaIST2,36.2% for Zhuhai Livzon′s ICS,37.7% for Dark horse′s IES and 36.8% for Shanghai Its prachanda.The results of susceptibility test: the reagent of IST2 had.the lowest resistance to josamycin,doxycycline,clarithromycin,ofloxacin and azithromycin,and had the better results than the other three kinds of reagents.Mycoplasma reagents form different companies had significant differences in resistance rates to five kinds of antibiotics.CONCLUSIONS The results suggest that when we cultivate clinical mycoplasma should be careful to select of the mycoplasma liquid culture identification kit.