4.Effects of physiological testosterone on transcription factor activity in human umbilical vein endothelial cells.
Hong JIN ; Wen-Bing QIU ; Geng PENG
Chinese Journal of Applied Physiology 2008;24(3):347-376
Cells, Cultured
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Flutamide
;
pharmacology
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Human Umbilical Vein Endothelial Cells
;
cytology
;
drug effects
;
metabolism
;
Humans
;
Proto-Oncogene Proteins c-myb
;
metabolism
;
Sp1 Transcription Factor
;
metabolism
;
Testosterone
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antagonists & inhibitors
;
physiology
;
Transcription Factors
;
metabolism
;
Tumor Suppressor Protein p53
;
metabolism
5.Study on the Quality Standard of Root of Urtica dioica and Different Harvest Periods
Hong QIU ; Bing DAI ; Yongxue PAN
China Pharmacy 2015;(24):3431-3433
OBJECTIVE:To study the quality standard of root of Urtica dioica and investigate the sample’s content in different harvest periods. METHODS:TLC was conducted to identify the scopoletin in the root of U. dioica with the developing agent of hexane-dichloromethane- ethyl acetate- formic acid (6∶10∶7∶1.2,V/V/V/V);alcohol-hot dipping was used to determine the con-tent;and the root of U. dioica in different harvest periods were comparatively researched. RESULTS:The lactone thin-layer chro-matogram feature was obvious,alcohol-hot dipping showed the contents of root of u. dioica was relatively high(RSD≤1%),the ex-tract content of root of U. dioica was the highest from late fall to early spring,and the stage was suitable excavation period. CON-CLUSIONS:The established quality standard can be used for accurately qualitative and quantitative,and studying different harvest periods is conducive to effectively control the quality of root of U. dioica.
6.Detection of antibodies to Epstein-Barr virus with ELISA based on fusion capsid protein
Qingfang QIU ; Yun WANG ; Bing LUO
Chinese Journal of Laboratory Medicine 2009;32(9):1002-1005
and 98.6% (436/442), respectively. Conclusion The ELISA based on fusion viral capsid proteins is sensitive, specific and accurate method for determining antibodies to VCA of EBV for both clinical diagnosis and epidemiology studies.
7.The effects of different centrifugal conditions on plasma B-type natriuretic peptide sample testing
Renjiang TANG ; Bing QIU ; Chunshu ZHENG
International Journal of Laboratory Medicine 2015;(4):516-517
Objective To investigate the plasma separation after samples to laboratory under different centrifugal conditions of b-type natriuretic peptide (BNP)the influence of the test results.Methods Randomly selected 20 cases of hospitalized patients at our hospital,the vacuum EDTA-K2 anticoagulant blood,respectively from several different centrifugal speed and time after centrif-ugal separation of plasma,the Siemens ADVIA Centaur CP on automatic chemiluminescence meter yet.Results Based on the same patients at the same time of blood specimens,under different centrifugal conditions and preparation of plasma BNP results meas-ured by comparative analysis,the centrifugal rotational speed of 3 000 r/min,the centrifugal time reach more than 5 min for the plasma specimens centrifugal conditions required by the test results and the kit standard preparation of plasma determination results difference has no statistical significance (P >0.05).Conclusion As soon as possible in order to determine the BNP as a result,es-pecially emergency specimens at night,when the centrifugal rotational speed and time to meet the 3 000 r/min and 5 min,can ob-tain accurate detection results,for clinical results as soon as possible for the BNP,diagnosis heart failure to win the time.
10.Analysis on the knowledge of non-communicable disease among general practitioners in the community
Yan QIU ; Ying LIU ; Wen REN ; Bing WANG ; Jingjing REN
Chinese Journal of Health Management 2016;10(3):188-191
Objective To better promote the health education and health promotion among patients with chronic disease, general practitioners (GPs) in the community should master the knowledge of non-communicable diseases (NCDs). This paper aimed to describe the current situation of GPs' knowledge about prevention and treatment of NCDs. Methods Self-designed questionnaires were issued to 1 077 GPs in community health service centers, and all GPs finished the questionnaire without assistance. The content of questionnaire includes the general information of GPs and six questions focusing on NCDs. Results Overall, 1 076 GPs completed the questionnaire, and the response rate was 99.9% (1 076/1 077). With regard to the six questions about NCDs, 90.4% (973/1 076) of GPs were well aware of the diagnosis of hypertension. Apart from that, the knowledge of diabetes mellitus (60.9%, 655/1 076), factors related to NCDs (70.2%, 755/1 076), the concepts of mental illness (52.0%, 560/1 076) and the clinical significance of tumor markers (87.9%, 946/1 076) among GPs were adequate, while more than half of the GPs did not know a lot about home oxygen therapy. Conclusion The mastering level of knowledge about prevention and treatment of NCDs among GPs varies that they need further improvement.