BACKGROUND:With the help of computer aided analysis technique, the prediction and analysis of the surgical treatment and rehabilitation of patients with femoral necrosis, which can realize the scientific operation and recovery of the patients after surgery, is a hot research topic in the field of international related research. OBJECTIVE:By studying the force deformation and stress distribution, fracture risk, and Interface’s biomechanical properties of the femoral head in different core decompression materials, we can provide the basis for choosing the appropriate core decompression material. METHODS:Based on nuclear magnetic resonance imaging images of six patients with different ages, the three-dimensional model and finite element analysis model were established, and the physiological load and four different types of materials (Actifuse, BioOss, Osteoset, Prodense) were set up. Finite element analysis software AnsysWorkbench was used to analyze the performance of the femur. RESULTS AND CONCLUSION:The maximum stress distribution law of the femur in the work process was obtained. With the increase of the Young’s modulus of the material, the maximum normal stress of the femoral necrosis region was decreased, but the biomechanical properties of the joint surface, such as sliding distance, friction stress and pressure, are increased. On the basis of comprehensive consideration of the biomechanical properties of the fracture risk and the junction, a suitable core decompression material (Osteoset) is recommended.

Objective To explore the changes of plasma endotoxin and D-lactate during intestinal mucosal barrier injury of acute pancreatitis.Methods Wistar rats were randomly divided into sham-operation(SO)group,and severe acute pancreatitis(SAP)group.Plasma endotoxin and D-lactate were determined at 1,3,6 and 12 hour after operations.Results The plasma endotoxin and D-lactate in SAP group increased obviously with time,and the difference betueen the 2 groups was significante(P

Objective To explore the role of apoptosis in the pathogenesis of acute pancreatitis(AP)and to (investigate) the effect of ligustrazin(LGT) on the apoptosis in acute pancreatitis .Methods Experimental animals were divided into three groups randomly: (1) AP group, (2) comparison group,and (3) therapy group. Pathologic score and TUNEL method were used to detect the pathologic mophology of pancreatic tissue and the pancreatic cell apoptosis index in the 3 groups. Results In the early stage of AP,the majority of cells are apototic and the degree of AP is slight.In the late stage of AP,the majority of cells show necrosis,and the degree of AP is severe.The (induction) of cell apoptosis can lessen the pathologic state of AP. Conclusions The degree of pathologic changes in AP can be lessened by the induction of cell apoptosis,but there is a definte time limit.

Objective To explore the role of inflammatory mediators in the pathogenesis of acute pancreatitis(AP).Methods Observation the relationship betweent the changes in serum TNF and IL-6 and(histopathology) of the pancreas in AP rats.Results(1)Rats in sham operation group survived throughout the experiment.Compared with of sham operation group,in AP group survival time shortened significantly(P

Traditional Chinese medicine teaching in general medical university was unsatisfied under the influence of many problems such as indefinite purpose,few class hours but many teaching contents,the different theoretical system between traditional Chinese medicine and western medicine,and theory divorced from practice etc.Therefore,the author suggested several measures that may help increasing teaching quality such as making definite course purpose and stimulating students' learning interest,enhancing training of imagery thinking,carefully electing teaching contents,integrating theory with practice and increasing clinical practice etc.

Objective To observe the clinical effects of treating Vetigo due to Vertebrobasilar Insufficient blood supply and hyperactivity of liver-Yang with Tianzhi Granules. Methods 118 cases were randomly recruited into a treatment group with 62 cases and a control group with 56 cases. On base of routine treatment, the treatment group was treated with Tianzhi Granules orally; and the control group was treated with Duflomed Hydrochloride orally. One course of treatment was 14 days. Results The total effective rates were 96.77%and 78.57% in treatment group and control group respectively. There was a significant difference between the two groups(P<0.05). The blood flow rat(BFR)of brain was improved in both groups, but the improvement of the BFR in brain was significantly better in the treatment group than the control group (P< 0.05). No adverse reaction was found in the treatment group. Conclusion Tianzhi Granules is effective and safe in treating Vetigo due vertebrobasilar insufficient blood supply.

Objective To explore the relationship between the sperm DNA integrity and ART result.Methods Employ improved SCD test for sperm DNA integrity assay.Meanwhile,statistical methods was used for data analysis.Results After compaed the infertility rate( IVF:77.4％ vs 84.8％,P =0.366;ICSI:90.1％ vs 92.1％,P =0.899),cleavage rate ( IVF:97.1％ vs 96.4％,P =0.958 ; ICSI:96.0％ vs 99.0％,P =0.845 ),high-quality embryo rate ( IVF:42.8％ vs 38.6％,P =0.485 ; ICSI:25.8 ％ vs 46.2％,P =0.002 ) and pregnancy outcome ( IUI:13.8％ vs 16.6％,P =1.000;IVF:35.3％ vs 73,7,P =0.150;ICSI:49.5％ vs 42.8,P =0.832) between the populations with normal and abnormal sperm DNA integrity during ART therapy,there is no statistical difference was found except high-quality embryo rate with ICSI therapy.Conclusion Sperm DNA integrity value can't be used to predict the ART outcomes independently.

Adult ; Aged ; Female ; Fistula ; surgery ; Humans ; Male ; Middle Aged ; Pectoralis Muscles ; transplantation ; Pharyngeal Diseases ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps

ObjectiveExpression and purification of human tumor necrosis factorα(hTNFα)fusion protein with a stretch of six consecutive histidine residues(6×His) in E. coli.MethodshTNFα fusion proteins with 6×His at N and C terminus were expressed by using E. coli expression vectors pET- 28a(+) and pET-22b(+). The His6 tag allows the expression fusion protein purified in one step by immobilized metal Ni2+ chelation affinity chromatcgraphy in native state. Results The two construct expression vectors were expressed in E. coli respectively, the former with high level as insoluble protein, account for 45% of the total bacteria proteins and not purified; the later 8% as soluble protein, and characterized by SDS- PAGE, Westren-blot. By using affinity chromatography through Ni2+ - IDA Sepharose 6B, 100ml induction culture had 0.4mg hTNFα-6×His fusion proteins. Its purifity reached 90 %. ConclusionThe purification expression product can possess TNF activity and reach 5.42×104U/mg.

Objective To explore the changes of Nogo-A mRNA and Nogo receptor(Ng-R) mRNA expressions in hippocampus of immature rats after febrile seizures(FS).Methods One hundred and twenty-seven male SD rats(15 days) were randomly divided into control group(immersed in 37 ℃ water,n=40)and hyperthermia treated group(immersed in 44.5 ℃ water,n=87).The latter was further divided into febrile control group(n=42) and FS group(n=45) according to whether seizures occurred or not.Each group was further divi-ded into 5 groups according to different therapies(1,3,5,7,10 times treatment).Then 5 cases of the 8 rats were randomly used to observe the expressions of Nogo-A mRNA,Ng-R mRNA in hippocampus by using reverse transcriptase polymerase chain reaction(RT-PCR).Three of the 8 rats were randomly used to observe the changes of neurons and mossy fiber sprouting(MFS) in hippocampus by Nissl and Timm staining.Results 1.No seizures occurred in normal control group.Seizures occurred in 2 rats of febrile control group.In FS group,various seizures occurred such as nodding spasms,tonic seizures,clonic seizures and tonic-clonic seizures,2 rats died of drowning and 3 rats died of status epilepticus.2.The expressions of Nogo-A mRNA,Ng-R mRNA in the immature rats′ hippocampus became up-regulated after the 7th and 10th seizure,significantly higher than those of the other 2 control groups(Pa