No abstract available.
beta 2-Microglobulin* ; Humans ; Kidney Failure, Chronic*

Serum (-2 microglobulin ((-2m) level was measured in 21 patients with severe chronic renal failure and 28 normal subjects (control group) aged from 18 to 30 years. Results: serum (-2m level of normal group: 1.527(0.610 mg/L. Serum (-2m level of patients: 22.3 (8.5 mg/L. There is a correlation between the serum (-2m level in patients with severe chronic renal failure and the other parameters such as serum Creatinin and Uric Acid and they are inversely correlated to GFR.
Kidney Failure, Chronic ; beta 2-Microglobulin

PURPOSE: Urinary N-acetyl-beta-D-glucosaminidase(NAG) and beta 2-microglobulin(B2M) is considered to be a marker of tubulointerstitial injury. The aim of this study was to examine the urinary levels of NAG and B2M in children with various renal diseases. METHODS: We studied 21 children(8.9+/-4.5 years, Male:Female=14:7) and they were divided into three groups: group I(steroid-sensitive nephrotic syndrome-4 patients), group II(various kinds of glomerulonephritis-4 patients), and group III(normal urinalysis or non-glomerular renal diseases-13 patients). RESULTS: Urinary NAG levels in groups I and II were significantly higher than those in group III(19.4+/-11.5 and 30.0+/-30.1 vs. 4.7+/-3.9, P=0.01), while urinary B2M levels did not differ among the 3 groups, although urinary NAG levels were positively correlated with urinary B2M levels(r=0.49, P=0.03). Urinary NAG and B2M levels were all correlated with proteinuria(r=0.79, P<0.001 and r=0.68, respectively, P=0.001) serum albumin(r=-0.72, P<0.001 and r=-0.57, respectively, P=0.01) and cholesterol(r=0.58, P=0.006 and r=0.56, respectively, P=0.013) levels. Conclusions: Urinary excretions of NAG and B2M are increased in children with steroid-sensitive nephrotic syndrome and various kinds of glomerulonephritis, suggesting tubular dysfunction might be present in these diseases.
Acetylglucosaminidase ; beta 2-Microglobulin ; Child ; Glomerulonephritis ; Humans ; Nephrotic Syndrome ; Urinalysis

This study was performed to evaluate the adsorption of beta2-microglobulin(beta2 M) by blood dialysis membrane materials which are polyethersulfone (PES), sulfonated polyethersulfones, (PES-SO3Na-I and PES-SO3Na-I ) in vitro incubated in human serum and radiolabeled beta2M (125I-beta2 M) solution respectively. In these experiments, the materials were incubated in 125I-beta2 M solution and human serum at the appointed time ranging from 15 minutes to four hours at 37 degrees C, and then the amounts of 125I-beta2M and serum beta2M adsorbed by materials were measured by radioimmunoassay (RIA). In the 125I-beta2 M system, amounts of 125I-beta2M adsorbed by the materials decreased in sequence of PES-SO3 Na-II > PES-SO3Na-I > PES. In the serum system, amounts of beta2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES-SO3Na-II > PES-SO3Na-I > PES. Sulfonated PES removes beta2M more than PES does and the adsorption of beta2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of beta2M may result in less beta2M available for incorporation into amyloid. The use of PES-SO3Na membranes lessens the likelihood of dialysis-related amyloidosis (DRA) development, which remains a major source of morbidity for patients treated with long-term hemodialysis.
Adsorption ; Amyloidosis ; blood ; Humans ; Polymers ; chemistry ; Renal Dialysis ; adverse effects ; Sulfones ; chemistry ; beta 2-Microglobulin ; blood

In multiple myeloma (MM), hyperdiploidy (HD) is known to impart longer overall survival. However, it is unclear whether coexistent HD ameliorates the adverse effects of known high-risk cytogenetics in MM patients. To address this issue, we investigated the clinicopathological characteristics of HD with high-risk cytogenetics in MM. Ninety-seven patients with MM were included in the study. For metaphase cytogenetics (MC), unstimulated cells from bone marrow aspirates were cultured for either 24 or 48 hours. To detect HD by interphase fluorescence in situ hybridization (iFISH), we assessed trisomies of chromosomes 5, 7, 9, 11, 15, and 17. Of the 97 MM patients, 40 showed HD. The frequency of co-occurrence of HD and high-risk cytogenetics was 14% (14/97). When the clinicopathological characteristics were compared between the two groups of HD with high-risk cytogenetics vs. non-HD (NHD) with high-risk cytogenetics, the level of beta 2 microglobulin and stage distribution significantly differed (P=0.020, P=0.032, respectively). This study shows that some of the clinicopathological characteristics of MM patients with high-risk cytogenetics differ according to HD or NHD status.
beta 2-Microglobulin ; Bone Marrow ; Cytogenetics* ; Fluorescence ; Humans ; In Situ Hybridization ; Interphase ; Metaphase ; Multiple Myeloma* ; Trisomy

To obtain recombinant human &beta;2-microglobulin (rh&beta;2M) with properties of good solubility and high purity from E. coli, prokaryotic expression conditions were optimized and protein purification was performed in this study. After testing the effect of different IPTG concentrations, temperatures and induction times on the production of rh&beta;2M, the optimum expression conditions were determined, i. e. joining IPTG to final concentration being 0.8 mmol/L and inducing time 6 h and at temperature of 25 degrees C. Under the optimum induction conditions, the ratio of soluble rh&beta;2M to soluble bacterial protein was 63.7%. After purified by Ni Sepharose 6 Fast Flow, the purity of rh&beta;2M achieved a greater value of 95%. Western blot analysis revealed that rh&beta;2M possessed the antigen property that specifically interacted with anti-&beta;2M antibody.
Blotting, Western ; Escherichia coli ; metabolism ; Humans ; Recombinant Proteins ; biosynthesis ; Solubility ; beta 2-Microglobulin ; biosynthesis

Female ; Humans ; Hyperbilirubinemia, Neonatal ; physiopathology ; urine ; Infant, Newborn ; Kidney Tubules ; physiopathology ; Male ; beta 2-Microglobulin ; urine

OBJECTIVE: To investigate the expression of HSP90 in bone marrow samples of multiple myeloma (MM) patients and explore its clinical significance. METHODS: Maxvision immunohistochemistry technique was used to detect the protein expression level of HSP90 76 MM patients and 29 normal healthy donors. The clinical characteristics of the patients were collected, and the correlation between the HSP90 expression and the clinical characteristics was analyzed. RESULTS: The count of MM patients with positive HSP90 protein was significantly higher than that of normal healthy donor, and there were no significant correlation between HSP90 expression and age, sex, hemoglobin (Hb), creatinine (CREA), blood calcium, lactate dehydrogenase (LDH), bone marrow plasma cell proportion and MM subtypes (P>0.05), but HSP90 expression was correlated with β CONCLUSION HSP90 protein was over-expressed in MM patients, and was correlated with β
Bone Marrow ; HSP90 Heat-Shock Proteins ; Humans ; Multiple Myeloma ; Prognosis ; beta 2-Microglobulin

To discern the morphological and functional changes of the kidney by percutaneous nephrostomy, unilateral nephrectomy was performed on 9 pigs followed by contralateral percutaneous nephrostomy about 4 weeks later. Renal function was measured every week after removal of nephrostomy tube till the animals were killed at 1, 2, 4 and 6 weeks. Microangiographic and histologic studies were done on the 9 kidneys of percutaneous nephrostomy and another 39 ex-vivo pig kidneys on which nephrostomy tracts were made under direct vision. The results are as follows : 1. Renal vascular injuries were developed mainly at the interlobular vessels in the beginning of dilatation. Interlobular, arcuate and segmental arteries were not torn but pushed away from the nephrostomy tract after dilation. The injured vessels did not heal completely till 2 weeks postoperatively. 2. Rental parenchyma were not removed but splitted away from the nephrostomy tract during dilatation. The nephrostomy tracts started to be replaced with fibrosis from the second week. From the forth week, tubules, vessels and glomeruli were visible in those fibrotic tracts. There were no differences of parenchymal and vascular injuries between the puncture to the calyceal fornix and that to the renal papilla. 3. Renal function decreased slightly at the second week postoperatively but they returned to normal levels thereafter. Beta 2-microglobulins of urine and blood were not detectable by enzyme immunoassay in the experimental subjects. Therefore, the nephrostomy tract constructed by splitting the parenchyme step by step doesn't change renal function.
Animals ; Arteries ; beta 2-Microglobulin ; Dilatation ; Fibrosis ; Immunoenzyme Techniques ; Kidney* ; Nephrectomy ; Nephrostomy, Percutaneous* ; Punctures ; Swine ; Vascular System Injuries

OBJECTIVETo investigate the renal function in newborns with birth asphyxia or intrauterine distress in the first week of life.

METHODSSixty full-term newborns born between June 2002 and February 2003 were assigned into three groups: Control group (healthy newborns), Intrauterine distress group (Apgar score > 7), and Birth asphyxia group without intrauterine distress (12 mild asphyxia and 8 severe asphyxia) (n=20 each). Urinary levels of alpha1-microglobulin (alpha1-MG), beta2-microglobulin (beta2-MG) and albumin (Alb) were detected by radioimmunoassay at 0-2, 3-4 and 6-7 days after birth.

RESULTSThe urinary levels of alpha1-MG, beta2-MG and Alb in the Asphyxia group were significantly higher than those in the Control group at all time points (P < 0.05), peaking at 3-4 days after birth. Statistically significant differences were found between the severely and mildly asphyxiated newborns for the urinary levels of alpha1-MG, beta2-MG and Alb at all time points (P < 0.05). There were no significant differences in the urinary levels of alpha1-MG, beta2-MG and Alb between the Intrauterine distress and the Control groups at each time point.

CONCLUSIONSBirth asphyxia may lead to renal glomerular and tubular impairments and it is speculated that the most serious impairment occurs at the 3rd and 4th days of life. The severity of renal impairments is associated with the degree of asphyxia. The renal function of the newborn appears to be normal following intrauterine distress.

Albuminuria ; urine ; Alpha-Globulins ; urine ; Asphyxia Neonatorum ; physiopathology ; Fetal Distress ; physiopathology ; Humans ; Infant, Newborn ; Kidney ; physiopathology ; beta 2-Microglobulin ; urine