Comet Assay

Serum levels of tumor maker CA 19-9 were quantified in healthy young and middle adults, including both genders. Participants had to have no sign of tumor or inflammatory diseases. Technically, level of CA 19-9 as quantifying by IRMA kid of CISbio International was reliable with%CV ranged from 0.1 to 5.4. Average value of CA 19-9 is 17.11+/-11.06. Level in high area was no more than 35.7 U/mL. Upper and lower marginal areas were 26.61 U/mL and 5.68 U/mL, respectively. There was no significant difference between two genders
Immunoradiometric Assay ; Serum

The human thyroglobulin (hTG) level was quantified in 31 serum samples taken from healthy people who aged from 18-55 years old. The standard curve, accuracy of technique and the serum hTG level of healthy people was determined. The results showed that the hTG quantified technique using immunoradiometric assay (IRMA) kits of CIS Bio International provided high accuracy. The average serum hTG level of healthy people is 7.35+/-6.99ng/ml; with common rage is 0.00 to 2ng/ml.
Thyroglobulin ; Immunoradiometric Assay

The serum concentrations of tumor markers of hTG and hCT in 33 healthy people were determined by the immunoradiometry assay (IRMA) has shown that the concentration of hTG and hCT was 7.35 +/- 6.99 mg/ml 5.74 +/-5.92ng/ml, respectively. There was no difference of concentrations of hCT and hTG between men and women.
Thyroid Neoplasms ; Immunoradiometric Assay

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

No abstract available.
Enzyme-Linked Immunosorbent Assay*

Aims: The present study is aimed at taxonomic characterization and isolation of active compound MS01 from Streptomyces sp. FACC-A032 which exhibited strong antitrypanosomal activity (IC50 0.02 μg/mL). Methodology and results: Isolate FACC-A032 was characterized based on its cultural, morphological, physiological and genomic properties. Isolate FACC-A032 was tentatively identified as Streptomyces sp. Biochemical analysis of diaminopimelic acid (DAP) isomer of whole-cell hydrolysates further confirmed the isolate FACC-A032 that contained LL-DAP isomer as species belonging to the genus Streptomyces. The inoculum for submerged cultures of isolate FACCA032 was prepared from cultures on ISP2 agar. After eight days of growth at 28  2 °C and 200 rpm in fermentation medium M3, fermentation broth was extracted with butanol and the crude extracts (solvent layer) were separated and dried in vacuo. Further studies were carried out to isolate the active compound from the culture extracts of isolate FACCA032. Using bioassay-guided isolation, crude extract was partitioned based on different polarity. After which, the resulting elutes were tested for antitrypanosomal activity. The active fraction was analyzed with HPLC-DAD analysis. Based on the analysis, major peak in the active fraction was collected using HPLC preparative. Active compound MS01 was isolated and structure elucidated using NMR spectroscopy. Conclusion, significance and impact of study: Bioassay-guided isolation techniques used in this study had discovered an active antitrypanosomal compound, staurosporine, from Streptomyces sp. FACC-A032. This is the first discovery of staurosporine, a protein kinase inhibitor, from Malaysian soil actinobacteria Streptomyces sp. Therefore, the study demonstrated the potential of Malaysian soil actinobacteria as antitrypanosomal therapeutic agent.
Biological Assay ; Actinobacteria

The quantification of CA 72-4 and CA 19-9 in normal people and patients with the peptic cancer was analysed and evalutated in the clinical application. The conentration of CA 72-4 and 19-9 in the normal people (disease free, tumor and infection free) and patients with the pepsic cancer (diagnosed by pathological anatomy and cytology) were quantified an compared.
Immunoradiometric Assay ; Peptic Ulcer ; neoplasms

No abstract available.
Enzyme-Linked Immunosorbent Assay* ; Korea*