BACKGROUND:Salvianolic acid B is an effective monomer component of Salvia miltiorrhiza, which has been shown in recent years to have neuroprotective role and to promote nerve recovery, but its mechanism is not clear. OBJECTIVE: To explore the protective mechanism of salvianolic acid B on Schwann cels in rats with spinal cord injury. METHODS: Schwann cels of Sprague-Dawley rats were cultured and divided into normal control group, model group, 10 μmol/L methylprednisolone group and salvianolic acid B group (0.1, 1, 10, 100 μmol/L). Models of Schwann cel injury induced by lipopolysaccharide were established in al the groups except the normal control group. After intervention, growth curve and proliferative activity of Schwann cels were detected, and protein and gene expressions of β-catenin and nuclear factor-κB were observed. RESULTS AND CONCLUSION: At 48, 72, 96 hours after intervention, the cel viability of the model group was significantly lower than that of the normal control group (P < 0.01). Compared with the model group, the cel viability of salvianolic acid B group (10 μmol/L) was significantly increased at 72 and 96 hours (P < 0.05); the expressions of nuclear factor-κB protein and mRNA in the methylprednisolone group and salvianolic acid B group were declined significantly (P < 0.01), but the expressions of β-catenin mRNA and protein in the salvianolic acid B group significantly increased (P < 0.05). These results suggest that salvianolic acid B improves the viability of Schwann cels which are stimulated with lipopolysaccharide, suppresses expression of nuclear factor-κB mRNA and protein, and promotes the expression of β-catenin mRNA and protein. Above may be one of the mechanisms which salvianolic acid B protects Schwann cels.

Objective To investigate the effects of the failure rate of laparoscopic apparatus with power through detecting the electrical properties before packaging it. Methods The laparoscopic apparatus with power used in the operation in January to June 2014 were randomly selected and divided into the control group and the observation group according to whether they underwent electricity performance detection. The control group did not receive testing of electrical properties before packing. The observation group tested the electrical conductivity of the single or double pole conductor, unipolar electric coagulation hook, bipolar electric coagulation forceps, the handle of electric knife by digital multimeter and the performance of the uterus rotary cutting machine by electric hysterectomy controller before packing. The difference of equipment in the number of failures, the type of failure, the number of cases and the satisfaction of medical personnel were observed between the two groups. Results In the observation group, 12 cases with unipolar and bipolar coagulation failure, 10 cases with electrocision handle failure, 2 cases with uterine rotary- cutting failure, 18 cases with adverse conductive or non-conductive, 6 cases with short circuit, which were significantly lower than those in the control group (36,25, 11, 48, and 26 cases), the differences were statistically significant (χ2=5.627-12.063, P<0.05). The satisfaction degree in the observation group was 94.74% (36/38), which was better than 76.32%(29/38) of the control group, the difference was statistically significant (Z=-2.267, P<0.05). Conclusions Testing the electric performance of laparoscopic apparatus with power before packing could reduce the incidence of intraoperative equipment failure rate, This method can provide safe and high quality surgical instruments.

Carotid artery stenosis is identified more and more common in clinical,and gradually become one of the causes of death and disablement.With the continuous development of medical technology,and medical device and apparatus equipment,surgical therapy and endovascular therapy have become one of the major principal methods in treatment of carotid artery stenosis.This article briefly discusses the current several processes and techniques of the surgical and endovascular treatment of carotid stenosis disease,and carries on the evaluation.

Objective To explore the application value of laparoscopic surgery in type 2 diabetes patients with acute cholecystitis.Methods 50 type 2 diabetic patients with acute cholecystitis were chosen as the research objects,the patients were divided into the observation group and control group according to the method of treatment, 25 cases in each group.Strict control of blood glucose levels in patients before operation,the observation group treated by laparoscopy,the control group were treated with conventional open surgery.Recorded the operation time and hemor-rhage,postoperative abdominal drainage,anal exhaust time,patient ambulation time and hospitalization time and other indicators.The patients were followed up to record the postoperative infection,occurrence of complications such as postoperative inflammation.Results There was no statistically significant differences between the two groups in oper-ation time(t =1.02,P >0.05).In the observation group,the intraoperative blood loss was (41.2 ±8.3)mL,postop-erative drainage volume was (107.5 ±16.4)mL,which were significantly lower than those in the control group,and the anal exhaust time was (1.7 ±0.3)d,total hospitalization time was (7.1 ±1.7)d,which were significantly shorter than those of the control group,the differences were statistically significant(t =3.43,3.78,3.43,4.21,all P <0.05).The observation group the complications included wound infection 1 cases,the complication rate was 4.0%, while in the control group,the complications included bile leakage,reflux esophagitis,incision infection,fat liquefac-tion,pulmonary infection and incisional hernia,the overall complication rate was 24.0%,there was statistically signifi-cant difference in the complication rate between the two groups(χ2 =4.54,P <0.05).Conclusion The laparoscopic surgery in the treatment of type 2 diabetic patients with acute cholecystitis has exact effect,and the patients recovery time is short,the complication rate is low.

Objective To study the effect of small incision plus tubular stomach on serum vascular endothelial growth factor(VEGF) and quality of life in patients with esophageal cancer.Methods 78 patients with esophageal cancer were randomly divided into two groups,the control group (n =36 cases) and the observation group (n =42cases).The patients in the control group were treated through the conventional operation,while the patients in the observation group were treated through small incision plus tubular stomach.Serum VEGF and quality of life were detected.Results Qualities of life in both two groups were improved after operation and it in the observation group was higher than that in the control group (t =4.36,P ＜ 0.05).Serum VEGF in both two groups were decreased after operation (t =26.11,12.28,all P ＜ 0.01).It in the observation group was lower than that in the control group (t =14.94,P ＜ 0.05).Conclusion Small incision plus tubular stomach can significantly improve serum VEGF and quality of life in patients with esophageal cancer.

Through viewing the employee medical insurance system, problems were found as below: ( 1) The content of system did not match the name of “urban workers” and “basic medical insurance”. (2) In financing system, some business units and participated people had heavy burden; personal accounts shunt exceed 60% of the total health insurance fund and constituted a reverse subsidy;retirees did not assume the obligation of payment while they could obtain unjust enrichment through the personal accounts of the possible. (3) System inefficient and risky fund cost overruns. (4) Insurance coverage is low within the system. Recommendations: (1) To build a unified national basic medical insurance based on merger residents and new rural cooperative medical insurance. (2) Abolish workers’ medical insurance system, put urban workers into the universal basic medical insurance. ( 3) Introduce “medical expenses surtax”, establish supplementary medical aid fund or direct universal basic medical insurance fund. ( 4) To develop commercial health insurance and build a comprehensive health care system.

Objective:To study clinical diagnostic value combined detecting plasma cardiac troponin T (cTnT)and N terminal pro B type natriuretic peptide (NT-proBNP)in children With heart failure (HF).Methods:A total of 73 child HF patients from our hospital Were enrolled as HF group,and another 75 healthy children from Well child clin-ic Were regarded as healthy control group.Concentrations of cTnT and NT-proBNP,and left ventricular ejection fraction (LVEF)Were measured and compared betWeen tWo groups.Receiver operator characteristic curve (ROC) Was used to analyze diagnostic sensitivity and specificity combined detecting cTnT and NT-proBNP.Results:Com-pared With healthy control group,there Were significant increase in concentrations of NT-proBNP [(2486.7±87.5)pmol/L vs.(3573.8±98.2)pmol/L]and cTnT [(0.03±0.01)ng/ml vs.(0.35±0.09)ng/ml],and significant decrease in LVEF [(70.8±5.2)% vs.(42.6±3.8)%]in HF group,P <0.05 all;multivariate Logistic regression analysis indicated that NT-proBNP Was an independent relative factor for LVEF in HF children (OR=1.006,P = 0.012);When NT-proBNP value Was 3200 pmol/L and cTnT value Was 0.08 ng/ml,their sensitivity and specificity Were 89.5% and 85.7% respectively in combined diagnozing child HF,and the area under ROCWas 0.795.Conclusion: Combined detecting cardiac troponin T and N terminal pro B type natriuretic peptide possesses high clinical diagnostic value in children With heart failure.

Objective To observe the efficacy of the combined treatment of Reduning,budesonide and erythromycin sequential therapy in the treatment of children with mycoplasma pneumonia and investigate its mechanism.Methods 78 children with mycoplasma pneumonia were randomly divided into two groups,each group 39 cases.The control group were given budesonide and erythromycin sequential therapy,and the observation group were given Reduning injection on the basis of the control group.Both groups were treated for 2 weeks.The clinical efficacy was observed,and before and after treatment,the pulmonary function was tested in the both groups.Results The total effective rate of the observation group was 94.87％,which was higher than that of the control group(86.62％)(x2=6.683,P＜0.05);The fever,cough improved,pulmonary rales disappeared,wheeze disappeared and hospital stay time of the observation group were significantly shorter than the control group(all P＜0.01);The FVC,FEV1 and PEF of the observation group were higher than those of the control group(all P＜0.05).Conclusion On the basis of the combined treatment of budesonide and erythromycin,the addition of Reduning injection can improve clinical effect and shorten the symptoms and signs improve time,improve lung function,chich may in association with regulating humoral immunity and reducing the levels of inflammatory cytokines.

ObjectiveTo explore the effect of health education on uncertainty in illness and hope level of patients with acute coronary syndrome.Methods100 patients with acute coronary syndrome were randomly divided into the control group and the observation group with 50 patients in each.The control group received routine treatment,the observation group was given health education based on routine treatment.After treatment Mishel Uncertainty in Illness Scale(MUIS)and Herth hope index(HHI)scale were used for effect evaluation.ResultsThere were no significant differences on MUIS and HHI scores between two groups before nursing intervention.But after nursing intervention,MUIS score was lower and HHI score was higher in the observation group than those of the control group.ConclusionsHealth education on patients with acute coronary syndrome can reduce the uncertainty in illness and improve the level of their hopes.

Burns ; pathology ; therapy ; Humans ; Prognosis ; Treatment Outcome ; Wound Healing