Matrix metalloproteinases(MMP) and Tissue Inhibitor of metalloproteinases(TIMP) can regulate the degradation and synthesis of extracellar matrix(ECM), and participate in many physiogical and pathological processes in vivo.They play important roles in periodical reconstruction of endemetrium.The unbalance of them are close by related to inflammatory endometrium hemorrhage and repairing.

Objective:To specify optimize condition of process for mixture extraction of three kinds of volatile oil and preparation of ? CYD inclusion compound of Yuxianling Granules.Methods: Taking weight of volatile oil to evaluate the extraction process and percentage recovery of inclusion compound, transfer rate of oil, inclusion rate of oil to evaluate the inclusion process. Orthogonal design was applied. Results: The optimum extraction condition was to add five times amount of water into the mixture of crude drugs, to soak for 90 min, to heat up to boiling and reflux 4h. The trituration method is suitable for the productive preparation of inclusion compound, the optimum inclusion condition was ? CYD: Volatile oil in proportion as 8∶1 was mixed, adding two times amount of water into the mixture and trituration 30 min.Conclusions: Above condition of process was the most rational in extraction and inclusion of three kinds of volatile oil.