Objective To explore the relationship between the level of synaptic monoamine transmitters and epilepsy and its severity.Methods The models of seizure were made with intraperiloneal injection. The concentrations of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) in palium(Pal), hippocampus (Hip) and corpus and striatum (Str) were determined with flurospectrophotometry. Results The severity of epilepsy was negatively correlated with the level of 5-HT in all three regions (P<0.05) with that of NE in Hip (P<0.01). The level of 5-HT of three seizure groups dropped significantly, the level of DA dropped significantly only in Str (P<0.01), and the level of NE dropped significantly only in Hip (P<0.01). Conclusion Monoamine transmitters in different regions closely affect the severity and sensitivity of epilepsy depends on the level of special transmitters in different brain regions.

Objective To explore the effect of alpha lipoic acid and Shuxuetong on oxidative stress and insulin resistance in patient with type 2 diabetes mellitus(T2DM)and to guide the clinical therapy for T2DM.Methods Totally 90 cases of T2DM pa-tients were sellected from Hainan Province Nongken Hospital.The patients were divided into Shuxuetong group(group A,n=30), alpha lipoic acid group(group B,n=30)and alpha lipoic acid and Shuxuetong group(group C,n =30).HOMA-IR,HOMA-β,and HOMA-ISI was calculated.SOD was determined by xanthine oxidase method.MDA was detected by Thiobarbituric acid method. The expression of IL-6 and TNF-αwas determined by ELISA analysis.Results Before treatment,SOD,MDA,IL-6,TNF-α,HO-MA-IR,HOMA-ISI,and HOMA-βwas no significant difference among the three groups(P >0.05).After treatment,all of which was significantly changed(P <0.05 and P <0.01).SOD,HOMA-ISI and HOMA-βin group C was significantly higher than that in group B(P <0.05),and which was significantly higher in group B than that in group A (P <0.05).MDA and HOMA-IR in group C was significantly lower than that in group B(P <0.05),and which was significantly lower in group B than that in group A (P <0.05).IL-6 and TNF-αin group C was lower than that in group A and group B(P <0.01),and which was no significant difference between group A and group B(P >0.05).Conclusion Alpha lipoic acid combined with Shuxuetong injection can significantly im-prove the oxidative stress state of T2DM patients,inhibit the inflammatory reaction,improve insulin resistance and patient′s sensi-tivity to insulin.Which was contribute to clinical treatment for T2DM.

ObjectiveTo describe the characteristics of treatment outcomes of multidrug-resistant tuberculosis （MDR-TB） patients enrolled in second-line treatment in Shanghai from 2017 to 2018， and to analyze the influencing factors of treatment outcomes. MethodsTotally 182 MDR-TB patients were analyzed by using data collected from the China tuberculosis management information system， the hospital's electronic medical record information system， whole genome sequencing results and a questionnaire survey， and logistic regression analysis was used to analyze the factors affecting the success of treatment. ResultsIn 182 MDR-TB patients， the success rate of treatment was 65.4%， the loss to follow-up rate was 8.2%， the mortality rate was 4.9%， the unassessable rate was 13.7%， and the drug withdrawal rate was 7.7%. The factors affecting the success of treatment in MDR-TB patients included age （35‒ years old， OR=5.28， 95%CI： 1.58‒17.59， P=0.007； 55‒ years old， OR=16.30， 95%CI： 4.36‒60.92， P<0.001） and compliance to medication （OR=0.55， 95%CI： 0.42‒0.72， P<0.001）. ConclusionThe treatment success rate of MDR-TB patients in Shanghai from 2017 to 2018 is significantly higher than the average level in China. Older patients and patients with less compliant are at higher risk of adverse treatment outcomes.

ObjectiveTo obtain the status of latent tuberculosis infection （LTBI） among tuberculosis （TB）-related health-care workers （HCWs） in Shanghai， and to explore the risk factors related to TB infection. MethodsA multi-center cross-sectional study was conducted by recruiting medical workers from multiple designated TB hospitals， centers for disease control and prevention， and community health service centers in Shanghai. Each subject was required to complete a questionnaire and to provide a blood sample for TB infection test. Univariate and multivariate analysis ware made in order to find risk factors relating to TB infection. ResultsA total of 165 medical workers were recruited， and the proportion of TB infection was 16.36% （95%CI： 11.49%‒22.76%）. Multivariate logistic analysis showed that clinical doctors and nurses （adjusted OR=9.756， 95%CI： 1.790‒53.188）， laboratory staffs （adjusted OR=78.975， 95%CI： 8.749‒712.918）， and nursing and cleaning workers （adjusted OR=89.920， 95%CI： 3.111‒2 598.930） had higher risk of TB infection. ConclusionThe overall LTBI prevalence among TB-related HCWs is low. However， working as doctors， nurses， laboratory staffs， nursing workers and cleaning workers are risk factors of TB infection. TB-related HCWs who work at hospitals are at risk of TB infection comparing to medical staffs who work outside hospitals.