1.Time-related effects of danazol treatment in experimentally induced endometriosis in the rat
Hui ZHENG ; Sicun HE ; Zhiquan BAI ; Zuoyan ZHOU ; Zineng WANG ; Hongyi LI
Chinese Journal of Pathophysiology 2001;17(5):422-424
AIM and METHODS:The purpose of the study was to characterize the time-related effect of Danazol therapy on endometriosis explant using the rat model. Endometriosis was induced in mature female rats. One group of treated animals as well as controls were sacrificed at 2,4,6 and 8 weeks after treatment at which time the explant was evaluated. RESULTS:Explant volume was significantly reduced in all treatment groups, and the effect was more significant in animals treated for 4 weeks than those treated for only 2 weeks. CONCLUSION:Danazol treatment can cause gradual regression of endometrial explant in a time-related manner.
2.Expression of IL-6 mRNA in endometrium with endometriosis
Hui ZHENG ; Hongyi LI ; Zineng WANG ; Yingshe ZHAO ; Li YU ; Sicun HE ; Zhiquan BAI ; Zuoyan ZHOU ; Ping YAO ; Yuechu WANG
Chinese Journal of Pathophysiology 1989;0(05):-
0.05), but in model rats it gradually increased at 2,4,6 and 8 weeks after endometriosis ( P
3.Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators.
Bingzhou HAN ; Yage ZHANG ; Xuetong BI ; Yang ZHOU ; Christopher J KRUEGER ; Xinli HU ; Zuoyan ZHU ; Xiangjun TONG ; Bo ZHANG
Protein & Cell 2021;12(1):39-56
Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function. However, contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been reported. Here we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NHEJ-mediated unidirectional targeted insertion in zebrafish facilitated by the CRISPR/Cas system. We demonstrated the feasibility of this strategy at sox10 and isl1 loci, and successfully achieved Cre-induced conditional knockout of target gene function and simultaneous switch of the fluorescent reporter, allowing generation of genetic mosaics for lineage tracing. We then improved the donor design enabling efficient one-step bidirectional knockin to generate paired positive and negative conditional alleles, both tagged with two different fluorescent reporters. By introducing Cre recombinase, these alleles could be used to achieve both conditional knockout and conditional gene restoration in parallel; furthermore, differential fluorescent labeling of the positive and negative alleles enables simple, early and efficient real-time discrimination of individual live embryos bearing different genotypes prior to the emergence of morphologically visible phenotypes. We named our improved donor as Bi-FoRe and demonstrated its feasibility at the sox10 locus. Furthermore, we eliminated the undesirable bacterial backbone in the donor using minicircle DNA technology. Our system could easily be expanded for other applications or to other organisms, and coupling fluorescent labeling of gene expression and conditional manipulation of gene function will provide unique opportunities to fully reveal the power of emerging single-cell sequencing technologies.
Alleles
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Animals
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CRISPR-Cas Systems
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DNA End-Joining Repair
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DNA, Circular/metabolism*
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Embryo, Nonmammalian
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Gene Editing/methods*
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Gene Knock-In Techniques
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Gene Knockout Techniques
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Genes, Reporter
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Genetic Loci
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Genotyping Techniques
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Green Fluorescent Proteins/metabolism*
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Integrases/metabolism*
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Luminescent Proteins/metabolism*
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Mutagenesis, Insertional
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Single-Cell Analysis
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Zebrafish/metabolism*