Objective:To establish an analytical method for HPLC fingerprint chromatography of Simiao Junyi ointment to provide basis for the quality control standard. Methods:The separation conditions were established to obtain the HPLC fingerprint chromatog-raphy of the main ingredients in Simiao Junyi ointment. The conditions were as follows:the chromatographic column was Ultimate C18-ODS(250 mm ×4.6 mm,5 μm), the mobile phase was acetonitrile-0.1% phosphate solution, the flow rate was 1.0 ml·min-1, the detection wavelength was 280 nm, and the column temperature was 35℃. The common peaks in the chromatography were analyzed for their belongings. Results:Gradient elution was performed under the above optimal separation conditions, the constituents in Simiao Ju-nyi ointment were separated from each other perfectly, and the optimal fingerprint chromatography was obtained. Though the methodolo-gy examination, the indicators such as precision, stability and repeatability of the method were all promising, and the fingerprint chro-matography could be seen clearly and was easy to be analyzed. The relationships between Simiao Junyi ointment and the common peaks of four medicinal materials in the fingerprint chromatography were preliminary determined, which provided important basis for the quali-ty control of Simiao Junyi ointment. Conclusion:The HPLC fingerprint chromatography of Simiao Junyi ointment can be used as an a-nalysis method for the quality control of Simiao Junyi ointment, which provides reference for the quality control standard for the finished product.

Objective To explore the effect of comprehensive intervention on social function and quality of life of patients with bipolar disorder (BD) in maintenance treatment period.Methods A total of 83 patients with BD were assigned into intervention group (n=41) and control group (n=42).Based on the original drug treatment,the intervention group was given comprehensive intervention,including BD health education,drug self-management,serf-monitoring of symptoms,interpersonal skills,coping and relaxation skills,learning to seek help from medical staff and others.The control group was treated with simple drug therapy.Before grouping,at the end of the third month and twelfth month,Young Mania Rating Scale (YMRS),Hamilton Depression Scale 17 items (HAMD17),Social Disability Screening Schedule (SDSS),Short Form 36 items Health survey Questionnaire (SF-36) were assessed.Results There were no differences in YMRS,HAMD,SDSS total score between the two groups before grouping(P＞0.05).The score of YMRS in the intervention group ((0.59±1.45) was lower than that in the control group(2.07±3.87) at the end of the 12th month,the difference was statistically significant (P=0.024).The score of SDSS in the intervention group (1.63± 1.77,0.78± 1.78) were statistically lower than that in control group (3.57± 1.78,2.74± 1.27) at the end of the 3th and 12th month(P＜0.01).There were statistically differences between the two groups in rolephysical(RP) (t=2.858,P =0.005),role-emotional (RE) (t =2.956,P =0.005),social function (SF) (t =4.163,P＜0.01),vitality (VT) (t =5.150,P＜ 0.01),mental health (MH) (t =2.830,P=0.007),general health (GH) (t=4.055,P＜0.01),difference in reported health transition (HT) (t=-2.092,P=0.042)at the end of 3th month.The differences were statistically significant in RE (t =3.290,P =0.001),SF (t =2.876,P =0.006),VT(t=5.831,P＜0.01),MH(t=4.839,P＜0.01),GH(t=3.752,P＜0.01) at the end of 12th month between the two groups.Conclusion On the base of medicine treatment,comprehensive intervention can effectively improve the social function and life quality of patients with BD in maintenance treatment period.