1.Comparision the effect on relievling stable angina between Shexiang Baoxin Pill and Isosorbide Dinitrate by singly dosing
Shugang LI ; Zuomin LI ; Qiongli ZHENG ; Jian LEI ;
Chinese Traditional Patent Medicine 1992;0(10):-
0.05). Conclusion: Both SXBXP and Isosorbide Dinitrate can significantly reduce the symptom of stable angina pectoris, but SXBXP is safer and has fewer side effects.
2.Effects of preincubation medium and other factors affecting in vitro fertilization rate of post-thawed genetically engineering mouse spermatozoa
Qin ZUO ; Tao FAN ; Cuiping ZHANG ; Wendong YANG ; Jinsong WANG ; Changfa FAN ; Zuomin LIU ; Zhengming HE ; Baowen LI
Chinese Journal of Comparative Medicine 2015;(1):45-49
Objective To discuss the effect of in vitro fertilization ( IVF) and mouse sperm cryopreservation , to establish a simple and economic frozen system for the genetically engineering mice preservation .Methods Sperm from genetically engineering mice were cryopreserved , IVF was performed using post-thawed sperm, then embryo transfer, to compare the effects of cryopreservation medium、age of male mice and sperm preincubation medium .Results Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , the fertility rates were from 82.49%to 91.43%, when HTF was used thawed-sperm preincubation in IVF , the fertility rates were from14.46%to 27.38%, there was a signification difference between PM and HTF sperm preincubation medium;10 to 35 weeks male genetically engineering mice sperm were succeed cryopreservation , and positive mice were procreated after 2-cell embryos were transferred;R18S3、CPM and CPA was used to freeze sperm , the fertility rates were 75.85%、88.89%to 94.27%, positive mice were procreated after 2-cell embryos were transferred;2-cell embryos after IVF were freezed , then thawed and positive mice were procreated after 2-cell embryos were transferred .Conclusion Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , genetically engineering mice sperm were succeed cryopreservation .
3. Influence of non-surgical periodontal treatment on the quality of life in chronic obstructive pulmonary disease patients with chronic periodontitis
Xuan ZHOU ; Zhiqiang LIU ; Wei LI ; Zuomin WANG
Chinese Journal of Stomatology 2019;54(3):151-156
Objective:
To evaluate the effects of periodontal therapy on the quality of life in chronic obstructive pulmonary disease (COPD) patients with chronic periodontitis (CP).
Methods:
In a randomized controlled pilot study, 60 COPD patients with CP were randomly assigned into three groups to receive scaling and root planing (SRP) treatment, supragingival scaling treatment and oral hygiene instructions only with no periodontal treatment, respectively. The scores of each patient′s quality of life at baseline, 1 year and 2 years, respectively, were evaluated by using the standardized St George′s respiratory questionnaire (SGRQ). The SGRQ was composed of three dimensions: symptoms, activity, and impact. A score was calculated for each section and a total summary score was also calculated. A high score indicated poor health and a decrease in the score indicates an improvement in quality of life.
Results:
There were no statistically significant differences among the three groups for age, gender, body mass index, smoking status, lung funtion, periodontal parameters and the SGRQ scores (including total, symptoms, activity and impacts scores) among three groups at baseline (all
4. Clinical significance of interleukin-6 and -8 in patients with chronic periodontal disease and acute exacerbation of chronic obstructive pulmonary disease
Yang YANG ; Wei LI ; Zuomin WANG ; Gengyun SUN ; Ping ZHOU ; Xiaolan HAN
Chinese Journal of Stomatology 2018;53(5):312-317
Objective:
To investigate the serum levels of interleukin (IL)-6 and -8 in patients with chronic periodontal disease and acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and the possible relationship between IL-6 and IL-8 with two diseases.
Methods:
A total of 40 cases of healthy subjects (control group 1) from graduate school of Anhui Medical University, and 120 cases (40 cases in each of the 3 groups) of eligible patients were collected, of which 40 were patients with chronic periodontal disease and AECOPD (experimental group) from Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University and Anhui NO.2 Provincial People
5. Screening of periodontal and salivary parameters in patients with frequent acute exacerbation of chronic obstructive pulmonary disease
Jitian WANG ; Zhiqiang LIU ; Tianyi ZHANG ; Yue CHEN ; Xuan ZHOU ; Guangxi LI ; Wenyan LIU ; Zuomin WANG
Chinese Journal of Stomatology 2019;54(6):410-415
Objective:
To screen the risk factors of patients with frequent acute exacerbation of chronic obstructive pulmonary disease (COPD) by detecting the clinical indicators of periodontitis and the level of bacterial and inflammatory markers in saliva.
Methods:
Thirty-eight COPD patients in their stable period were recruited and detected from Beijing Chao-Yang Hospital,Capital Medical University during December 2016 to May 2017. The periodontal index were recorded. The levels of inflammatory factors in saliva samples were examined by using enzyme linked immunosorbent assay (ELISA). The bacteria composition in the saliva samples were identified by using 16SrRNA gene pyrosequencing. All patients were followed up and monitored for acute exacerbation of COPD for 12 months. The patients were divided into frequent acute exacerbation group (≥2 times/year,
6.Quality evaluation of Guiqi tongmai mixture based on fingerprint and chemical pattern recognition
Yunzhi WANG ; Li GUO ; Zuomin WU ; Meng NING ; Zhenzhen YING ; Shaoju JIN ; Xiaotao YU ; Rui WANG
China Pharmacy 2022;33(20):2503-2507
OBJECTIVE To establish the fingerprint of Guiqi tongmai mixture and evaluate its quality in combination with chemical pattern recognition . METHODS Using narirutin as reference peak ,HPLC fingerprint of Guiqi tongmai mixture was established with Similarity Evaluation System of Chromatographic Fingerprint of TCM (2012 edition). Common peaks identification and similarity evaluation were conducted . Hierarchical cluster analysis (HCA),principle component analysis (PCA)and orthogonal partial least square -discriminate analysis (OPLS-DA)were performed ,and the value of variable importance in the projection (VIP) greater than 1 was taken as the standard to screen the differential markers affecting the quality of Guiqi tongmai mixture . RESULTS Nineteen common peaks in the HPLC fingerprints of thirteen batches of Guiqi tongmai mixture were calibrated ,and the similarities of them were 0.928-0.990;eight common peaks were identified ,including the peak 7(hydroxysafflor yellow A ),peak 8(albiflorin),peak 9(paeoniflorin),peak 10(calycosin-7-O-glucoside),peak 12(liquiritin),peak 14(ammonium glycyrrhizinate ), peak 16(narirutin)and peak 19(hesperidin). According to the results of HCA ,thirteen batches of samples could be clustered into three categories :S5,S9 and S 11-S13 were clustered into one category ;S4 and S 8 were clustered into one category ;S1-S3,S6- S7 and S 10 were clustered into one category . The results of PCA indicated that the accumulative variance contribution rate of the principle components 1-7 was 92.115%. The results of OPLS -DA showed that VIP values of narirutin ,paeoniflorin,liquiritin, ammonium glycyrrhizinate ,the components corresponding to peak 11 and peak 1 were greater than 1. CONCLUSIONS HPLC fingerprint of Guiqi tongmai mixture is established ,and it could be used for quality evaluation of Guiqi tongmai mixture by combining with chemical pattern recognition techniques . Six components such as narirutin are differential markers affecti ng the quality of Guiqi tongmai mixture .