1.Association between L-selectin gene P213S polymorphism and angina pectoris
Zunen XIA ; Yan LI ; Xiaoquan XIONG ; Kaihua MING
Chinese Journal of Clinical Laboratory Science 1985;0(04):-
Objective To explore the association between L-selectin gene P213S polymorphism and angina pectoris.Methods L-selectin gene P213S polymorphism in 138 patients with angina pectoris and 156 controls was detected by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP).The relationship between gene polymorphism of L-selectin and levels of serum lipids were also studied.Results L-selectin genotype frequencies of PP,PS,SS were 60.1%,36.3%,3.6% and 44.9%,48.1%,7.0% in angina pectoris group and control group respectively.Allele frequencies of P,S were 78.3%,21.7% and 68.9%,31.1% in angina pectoris group and control group respectively.There was significant differences of frequencies of genotype and allele of L-selectin P213S polymorphism between angina pectoris group and control group(P
2.The effect of nucleocapsid protein of severe acute respiratory syndrome-associated coronavirus on the expression of cyclooxygenase-2
Chengliang ZHU ; Yan LI ; Xiaohong YAN ; Yongqing TONG ; Ming WANG ; Zunen XIA ; Fang LIU
Chinese Journal of Infectious Diseases 2010;28(8):461-464
Objective To investigate the effect of nucleocapsid (N) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) on the expression of cyclooxygenase-2 (COX-2). Methods 293T cells were co-transfected with reporter plasmid pCOX-2-Luc containing the luciferase gene under the control of COX-2 promoter and plasmids carrying individual genes of SARS-CoV, and luciferase activity was measured. Expression of COX-2 mRNA and protein was measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results N protein of SARS-CoV enhanced COX-2 gene promoter activity, and upregulated COX-2 mRNA expression.COX-2 protein production in 293T cells was N protein concentration-dependent. Conclusion N protein of SARS-CoV could specifically activate COX-2 expression.
3. Analysis of 8 274 cases of new coronavirus nucleic acid detection and co-infection in Wuhan
Ming WANG ; Qing WU ; Wanzhou XU ; Bin QIAO ; Jingwei WANG ; Hongyun ZHENG ; Shupeng JIANG ; Junchi MEI ; Zegang WU ; Yayun DENG ; Fangyuan ZHOU ; Wei WU ; Yan ZHANG ; Zhihua LYU ; Jingtao HUANG ; Xiaoqian GUO ; Zhen CHEN ; Lina FENG ; Zunen XIA ; Di LI ; Tiangang LIU ; Pingan ZHANG ; Yongqing TONG ; Zhiliang XU ; Yan LI
Chinese Journal of Laboratory Medicine 2020;43(0):E016-E016
Objective:
To investigate the positive rate for 2019-nCoV tests and co-infections in Wuhan district.
Methods:
A total of 8 274 cases in Wuhan were enrolled in this cross-sectional study during January 20 to February 9, 2020, and were tested for 2019-nCoV using fluorescence quantitative PCR. Both respiratory tract samples (nasopharynx, oropharynx, sputum and alveolar lavage fluid) and non-respiratory tract samples (urine, feces, anal swabs, blood and conjunctival sac swabs) were collected. If both orf1ab and N genes are positive, they are classified as nucleic acid test positive group; if both orf1ab and N genes are negative, they are classified as negative group; if single gene target is positive, they are classified as suspicious group. Individuals were divided into male group and female group according to sex. At the same time, 316 patients were tested for 13 respiratory pathogens by multiplex PCR.
Results:
Among the 8 274 subjects, 2 745 (33.2%) were 2019-nCoV infected; 5 277 (63.8%) subjects showed negative results in the 2019-nCoV nucleic acid test; and 252 cases (3.05%) was not definitive (inconclusive result). The age of cases with COVID-19 patients and inconclusive cases was significantly higher than that of cases without 2019-nCoV infection (40 vs 56,