Objective To establish a HPLC method for the determnation of serum concentration of isoimperatorin in rats and study pharmacokinetic characteristic of isoimperatorin in rat after drug admiaistraction. Method The concentrations of isoimperatorin in rats were determined after drug administration and the pharmacokinetic parameters of isoimperatorin were estimated by DAS2.0 computer program. Result The concentration-time profiles were fitted with open two compartment model system. The main pharmacokinetic parameters were as follows:Tmax =(1.6?0.000)h, Cmax =(15.842?0.35)mg/L, AUC0-t=(80.761?10.03)mg/(L?h), AUC0-∞=(89.45?9.34)mg/(L?h), t1/2=(5.101?0.24)h. Conclusion The method is appropriate for the determination and pharmacokinetic study of isoimperatorin in rats after drug administration.

Objective To study the effect and the mechanism of sibelium on apoptosis and transcription of bcl 2 clan and interleukin 1? converting enzyme(ICE) gene in limbic system after rats' middle cerebral artery occlusion(MCAO).Methods MCAO model was made, and determined the dynamic changes on the cell counts of apoptosis, bcl 2 clan and ICE gene positive transcription in temple ortex, hippocampus and hypothalamus in the controls of normal saline,sibelium 50 ?g intervenient group and sibelium 100 ?g intervenient group during rats MCAO or reperfusion.Results The apoptosis counts of every times in sibelium 100?g intervenient group was significant lower than another two groups( P 0.05).The bcl 2 gene positive transcription cell counts at every times in sibelium 100?g intervenient group were significant higher than another two gruops( P

To know about the toxicity of Radix et Rhizoma Rhei objectively by reviewing the ancient records of toxicity of Radix et Rhizoma Rhei and modern research of its adverse effect.At the same time the reasons of the adverse effect of Radix et Rhizoma Rhei were discussed based on its experiments and clinical practice to provide some reference for its research and reasonable application.

The five flavours of traditional Chinese medicine(TCM)stand for five tastes,such as pungent,sweet,sour,bitter and salt.The five flavours not only stand for the actual tastiness of herbs,but also the effect characteristic of TCM according to traditional medical theory.So this kind of situation led to a disorder of the marking standardization of five flavours.So the authors think that we should mark herbs(or TCM)based on their true tastiness,and this way maybe help us to solve the problem and to research the relationship between the flavours and the effect of the herbs(or TCM).

Objective To compare eye expression recognition in stable outpatients with schizophrenia with that in normal controls and to explore the relationships between eye expression recognition and social functioning.Methods 107 schizophrenic outpatients and 66 normal controls matched in age,sex and years of education were assessed with Eye Basic Emotion Discrimination Task(EBEDT) and Eye Complex Emotion Discrimination Task(ECEDT).The patients were also assessed with Social Disability Screening Schedule (SDSS).Results The correct numbers were significantly lower for patients to identify basic emotions of eye expressions(13.2±3.8 vs16.0±2.6,P<0.01) and complex emotions of eye expressions(17.9±4.3 vs 20.6±3.5,P<0.01)than those for controls respectively;the correct numbers to identify anger(3.1±1.0 vs.2.1±1.2,P<0.01),fear(1.8±1.0 vs 1.3±1.0,P<0.01) and disgust(1.8±1.1 vs 1.4±1.2,P<0.05)for controls were higher than those for patients significantly.The correct numbers to identify total basic emotions(r=-0.335,P<0.05)and total complex emotions (r=-0.374,P<0.05)in eye expressions showed negatively correlated with the total scores of SDSS in the patients after controlling age and total score of PANSS.Conclusions The ability to recognize basic and complicated emotions in eye expressions in the outpatients with schizophrenia is lower than that in the controls. It shows positively correlated with social functioning moderately in the patients.

Objective:To investigate the attention deployment emotion regulation disorder and its neural mechanisms in major depressive disorder(MDD).Methods:Twenty-six MDD patients(MDD group) and twenty-nine healthy control individuals(control group) were enrolled in this study.The accuracy and reaction time were recorded in the attention deployment behavioral paradigm.Neuroscan 64 Brain Evoked Potentiometer was used to detect the N1 and P3 components of the above two groups of subjects.SPSS 16.0 software were used to analyze the data.Results:(1)In the accuracy of attention deployment paradigm, the group main effect was significant ( F=11.626, P=0.001), indicating that the MDD group (0.82±0.01) was significantly lower than the control group(0.89±0.01)( P=0.001). In the reaction time of attention deployment paradigm, the group main effect was significant( F=16.55, P<0.01), indicating that the MDD group (1 460.82±41.86)ms)was significantly higher than the control group(1 226.31±39.63)ms)( P<0.01). (2)In the event-related potential of attention deployment paradigm, the group main effect of N1 was not significant ( F=2.949, P=0.092), and the interaction between task and group was significant ( F=4.756, P=0.034), indicating that the N1 amplitude induced by the MDD group in the calculation task(-3.699±0.441)μV) was significantly smaller than that of the control group (-5.055±0.418)μV)( P=0.030). The group main effect of P3 was not significant ( F=1.165, P=0.285), and the interaction between task and image attributes was significant ( F=11.602, P=0.001), indicating that the P3 amplitude induced by negative images(2.757±0.438)μV) was significantly higher than that of neutral images (1.963±0.460)μV)( P=0.002). Conclusion:Patients with MDD have difficulty transferring attention in emotional strategies, and it occurs in the early stages.