OBJECTIVE:To explore the best way of drug application&taking in pharmacy through computer.METH-ODS:Ratio ordering method was applied to the high and low limit setups of drug stocks.RESULTS:The timely and dynamic high and low limit data setups through computer has successfully overcome the drawbacks of those from manual labor,thus more scientific and rapid drug application&taking was available.CONCLUSION:Ratio ordering method deserves to be generalization and application in drug application&taking by computer.

Objective:To explore the relationship between the polymorphisms in gene FGFR1, FGF10, FGFI8 and the nonsyndromic cleft lip with or without cleft palate (NS CLP) in Chinese population. Methods: Genomic DNA was isolated from peripheral lymphocytes of 75 patients with NS CLP and their parents and 75 unimpaired healthy children. The polymorphisms in FGFRI gene rs13317, p. E467K, p. M3691 and p. S393S, FGF10 gene rs1448037 and FGFI8 gene rs4043716 were detected by applying three-dimensional (3-D) polyacrylamide gel microarray technology. The data were performed using statis-tical analysis : the genotype frequenc+ y and allele frequency between patients with NSCL/P and control subjects were performed. Haplotype relative risk (HRR) , family based association test (FBAT) , and transmission disequilibrium test (TDT) in nuclear family were performed. Results: There were no poly-morphism in FGFR1 gene p. E467K, p. M369I and p. $393S site, the corresponding base was all G. The polymorphisms of rs13317 and rs1448037 were detected and their genotype frequency and allele frequen-cy showed no significant difference between 75 patients with NSCL/P and 75 normal children. TDT, HRR and FBAT were also no significant differences. The genotype frequency of gene FGF18 rs4043716 showed significant difference, but allele frequency were no significant difference. TDT, HRR and FBAT were also no significant difference. Conclusion: Our studies suggest an association between gene FGF18 rs4043716 and the NS CLP in Chinese population, and no association among gene FGFR1 rs13317, p. FA67K, p. M3691, p. S393S and gene FGF10 rs1448037.

Objective To observe the effects of transcranial magnetic stimulation combined with exercise training on fatigue and interleukin-1β (IL-1β),interleukin-9,interleukin-10,tumor necrosis factor-α and C-reactive protein (CRP) in patients with post-stroke fatigue (PSF).Methods A total of 90 patients with post-stroke fatigue were recruited and randomly divided into an observation group and a control group.Both groups were given routine exercise training,and the observation group was treated with transcranial magnetic stimulation in addition.Simplified Fugl-Meyer Motor Function Scale (FMA) and Fatigue Severity Scale (FSS) were used to assess the motor function of the limbs and fatigue of the two groups before treatment,4 and 8 weeks after treatment.At the same time,the levels of IL-1 β,IL-9,IL-10,TNF-α and CRP in the serum of the two groups were measured at the above time points.The correlation among the motor function,fatigue and cytokine levels was analyzed.Results There was no significant difference in the levels of IL-1β,IL-9,IL-10,TNF-α and CRP between the two groups before treatment (all P＞0.05);FMA score and fatigue improvement in the observation group were significantly better than those in the control group after treatment for 4 and 8 weeks (all P＜0.05);and serum levels of IL-1β,IL-9,IL-10,TNF-α and CRP in the observation group were significantly lower than those in the control group after treatment (all P＜0.05).Pearson correlation analysis showed that FMA scores of limbs were negatively correlated with serum levels of IL-1β,IL-9,IL-10,TNF-α and CRP (P＜0.05),and FSS scores were positively correlated with serum levels of IL-1β,IL-9,IL-10,TNF-α and CRP (P＜0.05).Conclusion Transcranial magnetic stimulation combined with exercise therapy can significantly improve the motor function and ameliorate fatigue of PSF patients.The therapeutic mechanism may be related to the reduction of serum levels of IL-1β,IL-9,IL-10,TNF-α and CRP.