1.A study on the risk factors of indirect inguinal hernia in pediatric patients
Chinese Journal of General Surgery 2001;0(07):-
Objective To evaluate the risk factors of pediatric indirect inguinal hernia. Methods A hospital-based case-control study was performed. One hundred and three indirect inguinal hernia cases with two -fold controls (206 cases) were enrolled. Parents were interviewed for their charateristics before and during conception. Unconditional logistic regression model was used for single factor and multivariate analysis to estimate odds ratios and their 95% confidence intervals by SAS 6.12 computer software. Results There were more males than females in the overall identified indirect inguinal hernia(Ratio=93.20%, P
2.Effects of rosuvastatin in homocysteine induced mouse vascular smooth muscle cell dedifferentiation and endoplasmic reticulum stress and its mechanisms.
Chang-Zuan ZHOU ; Sun-Lei PAN ; Hui LIN ; Li-Ping MENG ; Zheng JI ; Ju-Fang CHI ; Hang-Yuan GUO
Chinese Journal of Applied Physiology 2018;34(1):43-48
OBJECTIVE:
To investigate the effect of rosuvastatin on homocysteine (Hcy) induced mousevascular smooth muscle cells(VSMCs) dedifferentiation and endoplasmic reticulum stress(ERS).
METHODS:
VSMCs were co-cultured with Hcy and different concentration of rosuvastatin (0.1, 1.0 and 10 μmol/L). Cytoskeleton remodeling, VSMCs phenotype markers (smooth muscle actin-α, calponin and osteopontin) and ERS marker mRNAs (Herpud1, XBP1s and GRP78) were detected at predicted time. Tunicamycin was used to induce, respectively 4-phenylbutyrate(4-PBA) inhibition, ERS in VSMCs and cellular migration, proliferation and expression of phenotype proteins were analyzed. Mammalian target of rapamycin(mTOR)-P70S6 kinase (P70S6K) signaling agonist phosphatidic acid and inhibitor rapamycin were used in Rsv treated VSMCs. And then mTOR signaling and ERS associated mRNAs were detected.
RESULTS:
Compared with Hcy group, Hcy+ Rsv group (1.0 and 10 μmol/L) showed enhanced α-SMA and calponin expression (<0.01), suppressed ERS mRNA levels (<0.01) and promoted polarity of cytoskeleton. Compared with Hcy group, Hcy+Rsv group and Hcy+4-PBA group showed suppressed proliferation, migration and enhanced contractile protein expression (<0.01); while tunicamycin could reverse the effect of Rsv on Hcy treated cells. Furthermore, alleviated mTOR-P70S6K phosphorylation and ERS (<0.01)were observed in Hcy+Rsv group and Hcy+rapamycin group, compared with Hcy group; while phosphatidic acid inhibited the effect of Rsv on mTOR signaling activation and ERS mRNA levels (<0.01).
CONCLUSIONS
Rosuvastatin could inhibit Hcy induced VSMCs dedifferentiation suppressing ERS, which might be regulated by mTOR-P70S6K signaling.
Actins
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metabolism
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Animals
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Calcium-Binding Proteins
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metabolism
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Cell Dedifferentiation
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drug effects
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Cells, Cultured
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Endoplasmic Reticulum Stress
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drug effects
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Heat-Shock Proteins
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metabolism
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Homocysteine
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Membrane Proteins
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metabolism
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Mice
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Microfilament Proteins
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metabolism
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Muscle, Smooth, Vascular
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cytology
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Myocytes, Smooth Muscle
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cytology
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drug effects
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Ribosomal Protein S6 Kinases, 70-kDa
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metabolism
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Rosuvastatin Calcium
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pharmacology
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TOR Serine-Threonine Kinases
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metabolism
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X-Box Binding Protein 1
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metabolism