Acute leukemia is one of the most common cancers among children.The biological mechanisms leading to leukemia have not been fully clarified until now.Experiments were improved that most patients developing acute leukemia had abnormal chromosomes,including TEL/AML-l,BCR/ABL,PML/RAR-α fusion genes,which would be potential clinical biomarkers.Many genetic polymorphisms have effect on acute lymphoblastic leukemia susceptibility,which were containing genes involved in folate metabolism pathways,cytochrome P450,glutathione-S-transferase enzymes and quinone oxidoreductase-1.As the results of the studies,alcohol,tobacco,TNF-α and IFN-γ might be risk factors to the disease.Furthermore infection and IRF-3 were reported to reduce the occurrence of acute leukemia.Finally,as more is learned about the molecular pathology,it may be possible to develop new therapeutic agents which are specifically targeted to treat childhood acute leukemia.

The medical image registration between preoperative three-dimensional (3D) scan data and intraoperative two-dimensional (2D) image is a key technology in the surgical navigation. Most previous methods need to generate 2D digitally reconstructed radiographs (DRR) images from the 3D scan volume data, then use conventional image similarity function for comparison. This procedure includes a large amount of calculation and is difficult to archive real-time processing. In this paper, with using geometric feature and image density mixed characteristics, we proposed a new similarity measure function for fast 2D-3D registration of preoperative CT and intraoperative X-ray images. This algorithm is easy to implement, and the calculation process is very short, while the resulting registration accuracy can meet the clinical use. In addition, the entire calculation process is very suitable for highly parallel numerical calculation by using the algorithm based on CUDA hardware acceleration to satisfy the requirement of real-time application in surgery.
Algorithms ; Humans ; Imaging, Three-Dimensional ; Tomography, X-Ray Computed ; X-Rays

Objective To make a research on the expression of perlecan in the formation of epidural scar after posterior lumbar laminectomy in the rat.Methods Sixty Wistar rats were randomly and evenly divided into five groups:the experimental groups including A(the first week after surgery),B(the second week),C(the fourth week),D(the sixth week)and the control group.Laminectomy was performed on lumbar vertebra in each experimental group.The hematoxylin-eosin(HE)staining was used to observe the formation of epidural scar.Immunohistochemistry was performed to detect the perlecan located in the epidural scar.Western blot and RT-PCR were made to quantify the expression of the perlecan after surgery.Results According to HE,with prolonged,the scar tissue gradually formed.Immunohistochemistry demonstrated that the perlecan was expressed in the fibroblast.Western blot and RT-PCR showed the high level expression of perlecan in the experimental groups compared to the control group(P＜0.01).Furthermore,the expression of perlecan gradually increased with the prolongation of exposure time and had significantly statistical significance (P＜0.01)between the first three experimental groups.However,compared to the fourth week group,the expression of perlecan in the sixth week decreased obviously(P＜0.01).Conclusion Perlecan was expressed in the fibroblast of the epidural scar,and which plays an important role in the formation of epidural scar.

Objective To analyze the quality of life(QOL)of patients with acute stroke,and to explore the effects of early specialized rehabilitation on their QOL.Methods Seventy patients with acute stroke were randomly divided into a rehabilitation group(n =35)and a control group(n =35).The rehabilitation group received specialized rehabilitation treatment while the control group received self-rehabilitation.QOL we evaluated with the short form-36(SF-36)instrument before and after.Results Before treatment,QOL scores in the both groups were much lower than the norms for healthy people.The differences on each dimension of the SF-36 were not statistically significant between the two groups.The SF-36 scores of both groups improved during the experiment,but after 1 month and 6 months the improvement on each SF-36 dimension was significantly better in the rehabilitation group than in the control group.Conclusions The QOL of patients with acute stroke is significantly impaired,but specialized rehabilitation treatment is effective in improving it.

Objective:The purpose of this study was to assess the outcome of intestine fistula patients treated with home enteral nutrition(HEN) support before definitive operation.Methods:Fifty-eighty patients were divided into HEN support group and hospital enteral nutrition support group when their illness were stable and total enteral nutrition(TEN) were full resumed.All the patients were supervised by the members of nutrition support team.Days of TEN and hospital stay and medical cost was recorded.Plasma protein,body weight and body mass index were measured on the first day of full enteral nutriton and the day enteral nutrition was stop for operation preparation.Results:Nutritional status of the patients improved.There was no significant difference between two groups in nutritional status,complications and duration of TEN.The time patients in HEN group stayed at home was(61.11?27.71) d and in hospital(60.79?17.11) d.The cost in HEN group was less than that in hospital EN support group(140.21?61.2 vs 189.2?72.2 thousand Chinese dollar).Conclusion:Under the close supervise of nutrition support team,HEN is of the same safety and effect and can reduce the time of hospital-stay and medical cost when compared hospital EN.

Objective To study the effects of NEP1-40 on axon regeneration, motor function recovery of affected limbs and RhoA signal pathway in cerebral ischemia-reperfusion rats for exploring the possible mechanisms. Methods A total of 60 adult male Sprague-Dawley (SD) rats were equally divided into sham operation group (sham group), cerebral ischemia-reperfusion control group (control group), intra-lateral ventricle injection of PBS group (PBS group), and intra-lateral ventricle injection of NEP1-40 group (NEP1-40 group). The middle cerebral artery ischemia-reperfusion model (MCAI/R) was established by nylon monofilament occlusion method in rats. The changes in growth associated protein-43 (GAP-43) and RhoA expressions were determined by Western blotting technique. The motor function of affected limbs was tested by the "staircase test" of Montoya’s design. Results ① The expression of GAP-43 in NEP1-40 group was higher than that in the control and the PBS groups at 7 d and 14 d after MCAI/R, peaking at 7 d; ② The expression of RhoA in NEP1-40 group was significantly lower than that in the control and the PBS groups at each time point; ③ The results of "staircase test" in NEP1-40 group were much higher than those in the control group and the PBS groups at each time point after MCAI/R. Conclusion NEP1-40 can improve axon regeneration in cerebral ischemia-reperfusion rats and promote the recovery of motor function. The mechanism may be associated with the inhibition of RhoA signal pathway.

0.05). Conclusion: The IQ of children with epilepsy is lower than that of normal children .The epilepsy could impact the intelligence of children.

Role of chronic inflammation in type 2 diabetes and obesity has been recognized in the past few years. Examples of potent inflammatory cytokines are C-reactive protein (CRP),tumor necrosis factor-?(TNF-?) ,plasminogen activator inhibitor-1 (PAI-1) and interleukin-6 (IL-6). Inflammatory processes may have important roles in the development of type 2 diabetes,obesity and insulin resistance. This article reviewed the relationship between some inflammatory cytokines and diabetes, providing references for clinical diagnosis and treatment.

Objective To study the variable number tandem repeats (VNTR) polymorphism in intron 4 of the endothelial nitric-oxide synthase (eNOS) gene and its relationship with plasma NO in pregnancy induced hypertension (PIH). Method The VNTR polymorphism in intron 4 of the eNOS genes of PIH and healthy control pregnant women were detected by PCR. The plasma level of NO~(3-)/NO~(2-) was measured by spectrophotometer, which was used as the index of NO synthesis.Data were analyzed according to the different type of genotypes and allelic gene frequency. Results The PIH group showed significant higher frequency of VNTR 4a allele and 4a/4b+4a/4a genotype than those of the control (16.00% vs 7.50%,P

OBJECTIVE:To probe into the optimum condition and dosage of activated carbon in the preparation of arilin injection.METHODS:The impacts of activated carbon of different dosage at different temperature,different storage time and different pH value of preparation on the contents of arilin injection were investigated and analyzed statistically.RESULTS:Positive correlation was found between the descending degree of the contents of arilin and the dosage of the activated carbon(P