Objective To observe cocultured TAMs cell U937 and NSCLC cell A549 effecting on MCP-1, MIP-1 expression under different conditions in vitro. Methods The cell UM937 of different concentrations (0,1,2,4,8×105/ml) was respectively selected to coculture with the cell A549 0f certain concentration (1.0×105/ml) for 24 hours, in situ hybridization was carried out to detect MCP-1, MIP-1mRNA positive expressions in cell A549 of every concentration group. Under the condition that both concentrations of cell A549 and cell U937 are l.0×105/ml, the same method was used to measure MCP-1, MIP-1mRNA positive expressions in cell A549 in different periods (4h, 8h, 16h, 32h, 48h). Results The positive expression ratios of MCP-1, MIP-1mRNA in cell A549 significantly increased along with addition of the cell U937 concentration and delay of the cocultural time, apparently higher than that of primary cocultured group that the cell U937 was 0×105/ml and that of contrast group at every period (all P<0.05). Conclusion The mutual interaction between TAMs and NSCLC cells has closely positive correlation with MCP-1, MIP-1mRNA positive expressions in cell A549, and depends on cocultural concentration and time.

OBJECTIVE:To investigate the function change of intestinal CYP3A4 and P-glycoprotein (P-gp) under diabetic conditions and its mechanism. METHODS:Caco-2 cells were respectively added with 25, 50 and 100 μmol/L midazolam (CYP3A4 probe substrate) for incubation for 15,30,60,120,180 min;with 0.1,0.2,0.4 μg/ml rhodamine 123 (P-gp sub-strate)for incubation for 15,30,60,90,120 min to determine the substrate concentrations and incubation time. Caco-2 cells were added with insulin,glucose and fatty acid (palmitic acid and oleic acid) at different concentrations,and then the production of 1′-OH-midazolam,the metabolite of midazolam,and the intake of rhodamine 123 were determined,in order to investigated the ef-fect on the function of CYP3A4 and P-gp. RESULTS:The optimal substrate concentrations and incubation time were as follows as 50 μmol/L midazolam,0.1 μg/ml rhodamine 123 and incubation for 2 h. With the increase in the concentration of insulin,glucose and palmitic acid,the production of 1′-OH-midazolam and the activity of CYP3A4 reduced;the intake of rhodamine 123 in-creased,and the efflux transport function of P-gp decreased. Oleic acid had no significant effect on the production of 1′-OH-mid-azolam or the intake of rhodamine 123. CONCLUSIONS:Under diabetes condition,the increase of insulin,glucose and palmitic acid may reduce the function of CYP3A4 and P-gp,while oleic acid has no effect on the function.

Objective To investigate the risk factors for drug resistance‐tuberculosis(DR‐TB) ,and to establish a clinical risk predictive model .Methods A total of 126 cases of DR‐TB patients and 126 cases of non‐DR‐TB patients treated in our hospital from January 2014 to January 2015 were included in this study .The clinical data of these patients were collected .We used univariate and multivariate logistic regression analysis to determine the independent risk factors and established a risk predictive model .The calibration and discrimination of the model were assessed by the H‐L test and the area under the ROC curve ,respectively .Results Statistical analysis showed that the risk factors included previous treatment ,a duration of first treatment of more than 8 months ,ad‐verse effects of anti‐TB medication ,more than three TB foci in the lung and diabetes mellitus .H‐L statistic(χ2 =8 .760 ,P=0 .363) . The area under the ROC was 0 .826 ,95% CI(0 .766 ,0 .886) .Conclusion Logistic regression model established in the study can pre‐dict the incidence of DR‐TB with high prediction accuracy .

Objective To evaluate the effect of late course accelerated hyperfractionation radiotherapy(LCAHR) for esophageal carcinoma. Methods Six clinical trials on LCAHR for esophageal carcinoma were reviewed by means of Meta analysis . Results The 1 and 3 year survival rates in the LCAHR group derived from the fixed effect model were 2.39 (1.58～3.62) and 3.05(1.96～4.74) times higher than the conventional fractionation group. With the random effect model,the 1 and 3 year survival rates in the LCAHR group were 2.43(1.54～3.82) and 2.99(2.08～4.30) times higher than the conventional fractionation group.Conclusion For esophageal carcinoma,the better outcome of LCAHR makes it advisable in extensive clinical practice.

Objective: To study the effects of mechanical strain on the proliferation of human osteoblasts. Methods: Cloned MG 63 human osteoblasts were cultured and seeded at 10 5/well in 6 well Bioflex cell cuture plates. Subsequently, the cells were exposed to mechanical strain stimulation (at 6%, 12% and 24% elongation rate respectively) for 24 hours using Flexercell 3000 Cell Stretching Unit. MTT colorimetric method was used to assess the proliferation of the cells. Results: The proliferation of MG 63 cells was significantly increased 24 h after mechanical strain treatment( P

Objective To infer its transmission origin and route through studying the feature of molecular epidemiology of an HCV outbreak in Guangzhou.Methods Serum samples 192 cases in an HCV outbreak incident were analyzed for 2a and 6a subtypes.Appropriate genotypes were selected as control group.Informatics software were used in the evolutionary analysis to construct time scale trees and infer the origin time of HCV infection.Results Of 119 patients in the HCV outbreak,66 cases were 2a and 110 cases were 6a subtype.No other genotypes were found.The evolutionary analysis indicated the isolates of 2a group and 6a group both originated from 2 ～ 5 years ago.Both of 2a group and 6a group were in line with the characteristics of iatrogenic infection.Conclusion 2a and 6a were the main sub-genotypes in the Guangdong HCV outbreak investigation and they were originated from 2 to 5 years ago,which were in line with the characteristics of iatrogenic transmission.With the extension of time,a large number of cases accumulated and led to outbreak.

Objective To investigate the main pathogenic factors of fetal distress and its complications and to reduce perinatal mortality. Methods Two hundred and ten records and pathological slides in autopsy due to fetal distress were reviewed. Results Cord problem was the first cause of fetal distress followed by placental and maternal factors. Most fetal distress were caused by multi-factors. Among 210 cases,meconium aspirative syndrome was found in 170 cases (81%),amniotic fluid aspiration 36 cases (17.1%) and hypoxic syndrome 4 cases (1.9%). There were 59 cases complicated with aspirative pneumonia including 48 cases of meconium aspirative pneumonia and 11 with amniotic fluid aspirative pneumonia. The main complications were hypoxia-ischemic encephalopathy(HIE)(153 cases),diffused pulmonary hemorrhage(68 cases) and fatal malformations (27cases). Conclusion Fetal distress increases perinatal mortality when complicated with HIE,diffused pulmonary hemorrhage,pulmonary infection or fatal malformation

Objective In order to study the effect of the Fas system on normal peripheral blood lymphocytes(PBL)activated by allospecific antigen.Methods The isolated and purified lymphocytes werc subjected to one-way mixed lymphocyte culture.Fas antigen exprexsion on the surface of T lymphocytes freshly isolated and activated was detected dynamically by using flow-cytometry(FCM).The influence of the Fas system activated or not on the rate of lymphocytic apoptosis after mixed culture was investigated by using DNA electrophoresis and TdT-mediated dUTP nick end labeling(TUNEL).Results The Fas expression on the surface of activated T lymphocytes was increased and the rate of apoptosis of the grow with addition of anti-Fas monoclonal antibody was higher than that of the group without addition of antibody(P<0.05).Conclusion It was possible to dispose of the lymphocytes activated by specific antigen in vitro by anti-Fas monoclonal antibody.

Regulatory T cell (Treg) infiltration is an important mechanism for inhibition of tumor immune microenvironment. Treg is highly expressed in peripheral blood and tumor tissue of various cancer patients. A high expression of Treg may inhibit the antitumor effect of CD4 + CD8 + T lymphocytes or induce immunological resistance to tumor antigens among cytotoxic T lymphocytes(CTL) without producing acute or memory CTL responses, resulting in tumor escape from immuneattack and immune suppressionin in the tumor microenvironment.

Carcinoma, Adenoid Cystic ; Female ; Humans ; Laryngeal Neoplasms ; Middle Aged