Objective: To explore relationship between heart rate variability (HRV) and lower extremity arterial disease (LEAD) in aged patients with type 2 diabetes mellitus (T2DM) and their clinical significance. Methods: A total of 65 T2DM inpatients with age 60~65 years were enrolled. According to LEAD condition, they were divided into T2DM + LEAD group (LEAD group, n=36), T2DM without LEAD group (NLEAD group, n=29) ，besides, normal control group was set up (n=23). The 24h dynamic electrocardiography was performed. Indexes of HRV were measured; meanwhile, blood pressure, indexes of glucose and lipid metabolism, CRP and plasma adiponectin were measured in all groups. Correlation analysis and multi-factor Logistic regression analysis were performed. Results: (1) Levels of SBP, HbA1c, LDL-C, CRP in LEAD group and NLEAD group were significantly higher than those of normal control group (P<0.05). Compared with NLEAD group, there were significant increase in course of disease [(3.00±2.00) years vs. (7.50±4.00) years], SBP [(140.24±8.95)mmHg vs. (147.61±7.58)mmHg], HbA1c [(6.40±0.70)% vs. (7.15±2.05)%], plasma fibrinogen（Fg）[(2.57±0.51) g/L vs. (3.02±0.71) g/L], LDL-C[(2.27±0.50) mmol/L vs. (2.81±0.71) mmol/L] and CRP[(2.01±1.79) mg/L vs. (3.14±2.92) mg/L] in LEAD group, P<0.05 all; (2) SDNN, SDANN, VLF and plasma concentration of adiponectin in LEAD group and NLEAD group were significantly lower than that of normal control group (P<0.05 all); Compared with NLEAD group, there were significant decrease in other indexes except rMSSD in LEAD group, P<0.05 all; (3) Spearman rank correlation analysis indicated that LEAD was positively correlated with course of disease, SBP, 2hPG, HbA1c, LDL-C, Fg, CRP (r=0.311~0.760, P<0.05 all), and negatively correlated with adiponectin and HRV indexes (r=-0.597~-0.317, P<0.01 all); (4) Multi-factor Logistic regression analysis indicated that course of DM, SBP, HbA1c, LDL-C, CRP were independent risk factors for LEAD in aged T2DM patients (OR=2.932~14.404, P<0.05). Conclusions: 1. The course of DM, SBP, HbA1c, LDL-C, CRP, adiponectin and HRV are related with LEAD in aged T2DM patients ; 2. Decreased HRV is an independent risk factor of LEAD in aged T2DM patients.

Objective To study the expression changes of CD54 and CD106 in peripheral blood lymphocyte in patients with congest heart failure. Methods With FCM technique, the levels of CD54 and CD106 in lymphocyte from patients with CHF were measured , and those of patients with hypertension , patients with dilated cardiomyopathy and normal controls were measured at the same time. Cardiac function during heart failure episodes and remission stage was monitored by Color Doppler Echocardiography. Results Levels of CD54 and CD106 were significantly elevated in patients with hypertension , patients with active CHF and hypertension , patients with inactive CHF and hypertension when compared with those of normal controls. Levels of CD54 and CD106 were significantly elevated in patients of dilated cardiomyopathy , patients with active CHF and dilated cardiomyopathy , patients with inactive CHF and dilated cardiomyopathy when compared with those of normal controls. Levels of CD54 and CD106 in patients of CHF were elevated with the degree of CHF. There was significantly negative correlation between LVEF and CD54 of CHF. Conclusions CD54 and CD106 may use as the marker to monitor the progress of CHF.