Objective To express the molecular chaperone Acr2 protein of Mycobacterium tuberculosis(Mtb)in E.coli and analyze the function. Methods The recombinant plasmid pET-28a-Acr2 was transformed into competent E. coli BL21(DE3),and induced by IPTG. The expressed His-Acr2 protein was purified by Ni-NTA chromatography and SuperdexTM200 10/300 GL gel filtration chromatography to obtain Acr2 protein. The Acr2 protein was refolded by spontaneous refolding and reassembly after thermal denaturation(100 ℃ for 15 min)and chemical denaturation(8 mol/L urea,37 ℃ for 4 h).The secondary structure of Acr2 protein before and after denaturation-renaturation was detected by circular dichroism spectroscopy and non-denaturing SDS-PAGE,and the molecular chaperone function of Acr2 protein in vitro was detected by substrate binding assay. Results The purified Acr2 protein had the relative molecular mass of about 232 000,the purity of over 90%,and the concentration of about 2 mg/mL,which recovered its natural secondary structure after denaturationrenaturation,and formed stable complexes with the denatured malate dehydrogenase(MDH)at 48 ℃. Conclusion The Acr2protein can restore its natural molecular conformation with molecular chaperone activity in vitro after denaturation-renaturation treatment,providing a new strategy for the preparation of Mtb protein antigen with natural activity.

Objective To study the level of serum ICAM-1, VCAM-1, E-selectin, P-selectin, IL-6 and CRP in different Chinese medicine syndrome patients with carotid artery atherosclerosis. Methods Blood sample were collected from 176 patients with carotid artery atherosclerosis. The concentration of ICAM-1, VCAM-1, E-selectin, P-selectin, IL-6 and CRP were tested by means of ELISA. The subjects were divided into 3 groups according to the Chinese medicine syndrome, and the concentrations of inflammatory factors were compared among the 3 groups. Results Compared with control group, the level of inflammatory factors in the patients with carotid atherosclerosis was higher, and the levels of ICAM-1 and VCAM-1 in phlegm group were higher than blood stasis group and marrow deficiency group (P

Objective To analyze the antibiotic resistance of Branhemella catarrhalis strains isolated from sputum specimens of patients with lower respiratory tract infections from Linyi, Shandong Province, and to explore the relationship between bro genotypes of the strains and their resistance to antibiotic agents.Methods Sputum specimens were colleted from the patients with lower respiratory tract infections in Linyi People ’ s Hospital from the January 2010 to December 2014.The specimens were inoculated into 4 different disks for bacterial isolation and cultivation.β-lactamase detection and drug sensitivity tests were performed, and PCR coupled with restriction endonuclease analysis was employed for bro genotyping.χ2 test was used to compare drug resistance of strains with different bro genotypes.Results A total of 497 Branhemella catarrhalis strains were isolated in five years, among which 221 strains were isolated in winter.All strains were sensitive to ertapenem and chloramphenicol, and the resistance rates to amoxicillin/clavulanate and cefaclor were low (≤2.8%).The strains were highly resistant to compound sulfamethoxazole, erythromycin and ampicillin (47.6%-89.8%), and there was a trend of increasing resistance rates with the year, but no statistically significant difference was observed ( P >0.05 ) .β-lactamases was positive in 412 strains (82.9%), and all of these strains were positive for bro gene, and the resistances to erythromycin, compound sulfamethoxazole, levofloxacin and ampicillin were higher in bro positive strains than those in bro negative strains (χ2 =12.16, 16.18, 8.41 and 200.00,P<0.05).Among bro positive strains, 391 (94.9%) were of genotype bro-1, 21 (5.1%) were of genotype bro-2, and their resistance to antibiotic agents was not of statistical difference ( P >0.05 ).Conclusions Most of Branhemella catarrhalis clinical isolates are β-lactamase producing strains, and bro-1 is the most common genotype.Strains are highly sensitive to carbapenems, cephalosporins andβ-Lactamaseinhibitors, which can be recommended for the treatment of Branhemella catarrhalis-related respiratory tract infections.

Objective: To analyses the traditional Chinese medicine syndromes of chronic fatigue syndrome(CFS),by the way of variable cluster analysis.Methods: We researched 237 CFS patients,and recorded their symptoms,tongues and pulses.Then we used cluster analysis way to analyses these patients' clinical data.Results: These patients could be divided into 4 types: marrow deficiency syndrome,yin fluid deficiency syndrome,yang deficiency of spleen and kidney syndrome and overabundant liver-fire syndrome.The variable proportion is 61.68%.Conclusion: CFS is mainly produced by deficiency and/or excessive in traditional Chinese medicine pathology mechanism.And,the way of variable cluster analysis could help CFS patients' TCM syndrome differentiation.

Objective To analyze the internal relationship between phlegm stasis syndrome (痰浊内阻证) and blood stasis syndrome (血瘀证) in cases with carotid atherosclerosis. Methods The clinical data of 136 patients with atherosclerosis accompanied by phlegm stasis and blood stasis syndromes were collected from the investigation table of the 4 diagnostic methods in traditional Chinese medicine(TCM); the principal components of the clinical data were statistically analyzed, and the correlative relationships of these principal components were studied. Results There were two principal components of phlegm stasis syndrome, which were tz1 and tz2. And tz1 cumulated 42.645% to phlegm stasis syndrome, which could be considered as phlegm stasis in limbs and tunnel; tz2 cumulated 24.898% to phlegm stasis syndrome, which could be considered as phlegm stasis in the body that might impair the 7 orifices (such as eyes, ears, nose and mouth). And they could also be considered as special markers of phlegm stasis syndrome of carotid atherosclerosis. There were three principal components of blood stasis syndrome, which were xy1, xy2 and xy3. And xy1 cumulated 37.197% to blood stasis syndrome, which could be considered as blood stasis in the artery and tunnel; xy2 cumulated 21.627% to blood stasis syndrome, which could be considered as the blood outside the vessels located in the body and could obstruct tunnel and could not nourish muscle and skin; then, xy3 cumulated 13.685% to blood stasis syndrome, which could be considered as the blood stayed in the brain and could not nourish brain and might reflect as a special marker of blood stasis syndrome of carotid atherosclerosis. There was significant positive correlative relationship between phlegm stasis syndrome principal component 1 and blood stasis syndrome principal component either 1 or 2 (P

Objective To investigate the imaging diagnosis of acetabular dysplastic coxarthrosis. Method The imaging of the acetabular dysplasia was analyzed in 51 patients (87 hips). All but four patients were women. The age ranged from 22 to 78 years, and the mean age was 42.6 years. Pelvis radiographs were studied in all cases, and CT scan was performed in 18 cases and MRI in 10 cases. Results The fundamental signs on the plain film included shallow acetabulum, increased obliquity and insufficient coverage of the femoral head by the acetabulum. The CE angles were determined in all the cases, ranging from -10? to 30?, mean 12.9?. The charp angles ranged from 35? to 67?, mean 45.3?. The secondary osteoarthritis were revealed in 75 hips (84.2%). 54 hips (62.1%) had cyst like lesions in weight bear area of the acetabulum or femoral head. 19 hips had subluxation of femoral head. CT and MRI displayed small cystlike lesions and forward shift of the femoral head, which could not be shown on plain film, respectivly in 13 and 10 hips. Conclusion The cyst like lesion of subarticular region is a common sign in acetabular dysplastic coxarthrosis. CT or MRI can show the early osteolytic lesion and forward subluxations.

OBJECTIVE To analyze the current situation of the hospital.and the community-acquired infections due to Staphylococcus aureus(SAU) and provide the reference for clinical reasonable use of drugs.METHODS The clinically isolated S.aureus strains during 2004-2006 were collected,cultured and identified.Their susceptibilily to 16 kinds of antibacterials was detected by K-B or MIC and WHONET5 software was used to analyze the result.RESULTS From 440 S.aureus strains,the meticillin-resisstant S.aureus(MRSA) was 260 accounting for 59.1%;the meticillin-sensitive S.aureus(MSSA) was 180 accounting for 40.9%.The resistant rate of SAU to penicillin G was the highest(85.5%),that to vancomscin was 0.CONCLUSIONS MSSA still keeps fairly good sensibility to most antibacterial medicine,but MRSA shows the multidrug resistance,except to vancomycin.For this studying the resistance mechcnism of Staphylococcus and continuously detecting the emergence of vancomycin-intermediat and vancomycin-resistante S.aureus have a significant clinicil importance.

OBJECTIVE To investigate the distribution and resistance of Pseudomonas aeruginosa isolated during Jan-Dec 2006.METHODS The clinically isolated P.aeruginosa strains were collected cultured and identified by paper diffusing method or trace dilution method(MIC),the results were evaluated according to the relevant documents of NCCLS of USA.RESULTS The in vitro susceptibility test of 244 P.aeruginosa isolates to 16 kinds of antibacterials indicated the resistance rate to SMZ was the highest(98.8%);then to minocycline,tetracycline and ticarcillin/clavulanicacid(70.1%,58.6% and 54.5% respectively).CONCLUSIONS To strengthen the continuous survezillance of drug resistance of P.aeruginosa,to sum up the resistance rules of main pathogens of departments in hospital and to reduce of production of resistant bacterica have the important significance.

OBJECTIVE To understand the antibiotic resistance of Pseudemonas aeruginosa isolated from ICU and give advices to clinicians.METHODS The antibiotic susceptibility of P.aeruginosa isolated from ICU to 12 kinds of antibiotics were tested by disc diffusion method.RESULTS The antibiotic susceptibility of 472 P.aeruginosa strains to imipenem,piperacillin/sulbactam,piperacillin,amikacin,ceftazidime,tobramycin,cefepime,ciprofloxacin,ceftriaxone,gentimicin,aztreonam and cefotaxime were 82.2%,74.94%,69.92%,64.87%,59.95%,52.93%,49.88%,49.65%,44.50%,41.92%,38.17%,and 35.60%,respectively.CONCLUSIONS P.aeruginosa isolated from ICU of our hospital is suscepitable to imipenem,piperacillin/sulbactam,piperacillin,amikacin and the cephalosporins,but show lower susceptibility to other antibiotics.

OBJECTIVETo investigate the effects of Naokang Erhao decoction on the cognitive ability and the expression of Caspase-3, Bax and Bcl-2 proteins in hippocampus of type 2 diabetic rats.

METHODThe diabetes mellitus (DM) rat model was produced by intraperitoneal injection of streptozotocin and fed with high fat and sucrose diet. The Naokang Erhao-treated rats were intragastrically given different doses of Naokang Erhao, whereas the control and DM model groups were given double distilled water for 4 consecutive weeks. Learning and memory abilities of rats were tested with the Morris water maze. The expression of Caspase-3, Bax and Bcl-2 proteins in hippocampal CA1 region was measured by immunohistochemistry.

RESULTBoth escape latency and swimming distance of type 2 DM rats were significantly prolonged in comparison of those in normal control (P < 0.01), and swimming time in the platform of previous quadrant was significantly shorter in model group (P < 0.01). Furthermore, the expression of Bcl-2 protein was decreased, while Caspase-3 and Bax in the hippocampus were increased compared with the control group (P < 0.01). Four weeks of treatment with Naokang Erhao decoction remarkably improved the learning and memory abilities of DM rats, increased the expression of Bcl-2 and decreased the expression of Caspase-3 and Bax in hippocampal CA1 region of model rats (P < 0.01 or P < 0.05).

CONCLUSIONNaokang Erhao decoction may inhibit apoptosis by increasing the expression of Bcl-2 and reducing the expression of Caspase-3 and Bax in the hippocampus, and this may be one of the mechanisms by which Naokang Erhao decoction improves cognitive ability in DM rats.

Acetophenones ; pharmacology ; Animals ; Apoptosis ; drug effects ; Benzoates ; pharmacology ; Bridged-Ring Compounds ; pharmacology ; CA1 Region, Hippocampal ; drug effects ; metabolism ; Caspase 3 ; drug effects ; metabolism ; Cognition Disorders ; drug therapy ; metabolism ; Diabetic Neuropathies ; drug therapy ; metabolism ; Diterpenes, Abietane ; pharmacology ; Drug Combinations ; Drugs, Chinese Herbal ; pharmacology ; Glucosides ; pharmacology ; Male ; Maze Learning ; drug effects ; Proto-Oncogene Proteins c-bcl-2 ; drug effects ; metabolism ; Pyrazines ; pharmacology ; Rats ; bcl-2-Associated X Protein ; drug effects ; metabolism