OBJECTIVE To understand the distribution and antibiotic resistance of E.coli isolated from our hospital and offer suggestions for clinic.METHODS The antibiotic susceptibility to 21 kinds of antibiotics were tested by K-B method and analyzed by Whonet software.RESULTS The antibiotic resistance of 301 E.coli strains to ampicillin,quinolones,piperacillin,gentamicin,cefazolin,cefuroxime,ceftriaxone,ceftazidime,aztreonam,cefepime, ampicillin/sulbactam were 83.39%,69.44%,62.79%,44.19%,42.86%,36.88%,35.22%,31.23%,29.90%,29.57% and 26.91%,respectively.CONCLUSIONS E.coli isolated from our hospital show high resistance to several kinds of antibiotics.The drugs should be choosed reasonably according to their antibiotic suscepitibity results.

OBJECTIVE To explore the gene gyrA mutation in nalidixic acid-resistant Salmonella paratyphi A(SPA) in Shaoxing area.METHODS Fifty-two strains of SPA gene were assessed by amplification and purification and the PCR products were detected by two directional sequencing methods.The sequences of DNA gyrase A were compared and analyzed with the OMIGA2.0 software,and the gene gyrA mutation was identified.RESULTS The mutation in nalidixic acid-resistant SPA was discovered in No.83 or No.87 animo acid site of gyrA quinolones resistant determinate region(QRDR),and the mutation of 51 strains occurred in No.83 site,1 strain occurred in No.87 site.The simultaneous mutation of two sites was not discovered,No mutations were found in other sites of gyrA QRDR.CONCLUSIONS No.83 site mutations of gene gyrA has a direct relation to nalidixic acid-resistant SPA.No.87 site mutation of gene gyrA might also cause nalidixic acid-resistantce.The nalidixic acid-resistant SPA is mainly mutated in No.83 site by TCC→TTC(Ser→Phe) in Shaoxing area.

Objective To compare the sleep quality of cerebral infarction patients with different types in sub-acute phase. Method The patients with cerebral infarction targeted to the inclusion criteria were divided into the phlegm-heat group and non-phlegm-heat group according to the syndrome differentiation of TCM. After routine treatment of western medicine for 4 weeks, the sleep quality of patients were evaluated with Pittsburgh Sleep Quality Index (PSQI) during the sub-acute phase. Result The scores of subjective sleep quality, sleep duration, sleep efficiency and overall PSQI score in the phlegm-heat group were obviously higher than that in the non-phlegm-heat group (P

Objective To observe the survival quality of cerebral infarction patients in sub-acute seizure phase, who were in different TCM syndrome types. Methods The patients with cerebral infarction in acute seizure phase targeted to the inclusion criteria were divided into the heat group and non-heat group by TCM syndrome differentiation. After 4 weeks of routine treatment with western medicine, the survival quality of patients was evaluated with Health Questionnaire Short Form-36 (SF-36). Results In all dimensions of SF-36, the scores of social function, mental health, role-emotional dimensions in the heat group were obviously lower than those in the non-heat group (P

Objective To compare the sleep quality and traditional Chinese medical (TCM) syndrome between men and women with cerebral infarction. Method The patients with cerebral infarction enrolled were divided into men patients group and women patients group. After routine treatment of western medicine for 2 weeks, the sleep quality of patients were evaluated with Pittsburgh Sleep Quality Index (PSQI), and the TCM syndrome types were determined by the Diagnostic Criteria of Syndrome Differentiation of Stroke. Result The subjective sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbance, use of sleep medication, day-time dysfunction and the overall PSQI scores were higher in the women patients group than that in the men patients group (P

For all the time the book Symptomatic Figures of Lip and Tongue(Chunshe Zhenghou Tu)was described as written by Li Jun of Qing dynasty. We made studies on historical records on Li Jun and the features and contents of this book, and proved that Symptomatic Figures of Lip and Tongue was not written by him, but a Chinese medical book written by Japanese.

Objective: To analyses the traditional Chinese medicine syndromes of chronic fatigue syndrome(CFS),by the way of variable cluster analysis.Methods: We researched 237 CFS patients,and recorded their symptoms,tongues and pulses.Then we used cluster analysis way to analyses these patients' clinical data.Results: These patients could be divided into 4 types: marrow deficiency syndrome,yin fluid deficiency syndrome,yang deficiency of spleen and kidney syndrome and overabundant liver-fire syndrome.The variable proportion is 61.68%.Conclusion: CFS is mainly produced by deficiency and/or excessive in traditional Chinese medicine pathology mechanism.And,the way of variable cluster analysis could help CFS patients' TCM syndrome differentiation.

ObjectiveTo investigate the anxiety and its effect on the sleep quality in patients with cerebral infarction and sleep-disordered breathing. Methods149 cerebral infarction patients were divided as 95 without sleep-disordered breathing and 54 with sleep-disordered breathing. They were evaluated with Hamilton Anxiety scale (HAMA) and Pittsburgh Sleep Quality Index (PSQI). ResultsPatients with sleep-disordered breathing showed higher HAMA overall score and factor scores of somatic anxiety and psychic anxiety（P<0.001）. In the factors of somatic anxiety, the scores of sensing, cardiovascular, gastrointestinal and autonomic nerve system were higher in patients with sleep-disordered breathing than those without sleep-disordered breathing （P<0.05）. In the factors of psychic anxiety, the scores of anxious mood, tension, fears, insomnia, depressed mood were higher in patients with sleep-disordered breathing（P<0.05）. PSQI overall score and all factor scores were also higher in patients with sleep-disordered breathing（P<0.001）. HAMA overall score as well as psychic anxiety and somatic anxiety scores were closely correlated with the PSQI overall score （P<0.001）. Somatic anxiety score was correlated with subjective sleep quality, sleep latency, sleep disturbance and daily dysfunction scores （P<0.05）. Psychic anxiety score was correlated with all the dimension scores of PSQI （P<0.01）. ConclusionCerebral infarction patients with sleep-disordered breathing appeared anxiety and worse sleep quality than patients without sleep-disordered breathing. Anxiety is closely related with sleep quality.

Objective To investigate the relationship between carotid atherosclerosis （CAS） and cognitive impairment, stroke-specific quality of life of patients with cerebral infarction. Methods Patients enrolled were divided into mild-to-moderate CAS group and severe CAS group after carotid ultrasound. They were assessed with Mini Mental State Examination (MMSE) and Stroke Impact Scale (SIS) Version 3.0. Results The scores of attention/calculation, comprehension, and total of MMSE, the mobile ability and hand function dimensions of SIS were all lower in severe CAS group than in mild-to-moderate CAS group （P<0.05）. Conclusion Carotid atherosclerosis may further impair the cognitive function and quality of life of cerebral infarction patients.

Objective To determine the clinical characteristics of comorbid anxiety and depression after cerebral infarction. Methods All the patients enrolled were divided into post-stroke comorbid anxiety and depression (PSCAD) group (84 cases) and the control group (121 cases). Their condition of smoke, alcohol intake, education background, carotid plaque and brain blood vessels were investigated, and they were evaluated with Hamilton Anxiety Scale (HAMA), Hamilton Depressive Scale (HAMD), Barthel index, and Mini-Mental State Examination (MMSE). Results There was not significant difference in condition of smoke, alcohol intake, education background, carotid plaque and brain blood vessels between these groups （P＞0.05）, but were in total scores and all the factors scores of HAMA and HAMD （P＜0.05）. PSCAD group showed more obvious anxious mood, depressive mood, tension, insomnia, cognitive impairment and autonomic nervous symptoms. Psycho-anxiety, soma-anxiety, despair, guiltiness, decreased interest were also more significant in the PSCAD group. The scores of MMSE and Barthel index were also impaired. Conclusion The cognitive function and daily living ability are worse in the patients with PSCAD. The prominent symptoms includes: anxious mood, depressive mood, tension, insomnia, cognitive impairment, autonomic nervous symptoms, soma-anxiety, despair, guiltiness, decreased interest.