1.Orienting medical engineer to enhancing efficiency
Daming PENG ; Zongxiang HUANG ; Manfang LIU
Chinese Medical Equipment Journal 1989;0(03):-
With the maintenance, purchase and utilization analyzed, the medical engineer is oriented to enhancing the efficiency of the hospital and strengthening the construction of medical engineering department.
2.Overexpression of ephrinB2 in canine periodontal ligament stem cells regulates osteogenic differentiation
Yumiao Liu ; Jinxin Yang ; Shaoyue Zhu ; Yulin Ma ; Hanping Xia ; Meng Zhou ; Shulei Yang ; Taining Huang ; Kexin Ding ; Zongxiang Liu
Acta Universitatis Medicinalis Anhui 2023;58(3):423-428
Objective:
Investigate osteogenic differentiation of canine periodontal ligament stem cells ( cPDLSCs) via over-expression ephrinB2 in cPDLSCs.
Methods :
cPDLSCs were isolated from the premolars and molars of Beagle.After transfected with EfnB2-GFP-Bsd and GFP-Bsd empty Vector,cPDLSCs were induced to osteogenic differentiation.Western blot was used to invest the expression of ephrinB2 protein.The effect of osteogenic differentiation of EfnB2-cPDLSCs and Vector-cPDLSCs were analyzed by RT-PCR , CCK-8,Alizarin-red S staining and ALP.
Results:
There was no significant difference in cell proliferation between EfnB2-cPDLSCs and Vector-cPDLSCs.While EfnB2-cPDLSCs displayed an enhanced ALP activity and more prominent mineralized nodules compared with Vector-cPDLSCs.The odonto-/ osteogenic genes in EfnB2-cPDLSCs were also highly enhanced.
Conclusion
The results of our study indicated that ephrinB2 gene-transfected cPDLSCs showed enhanced osteogenic differentiation.