Object To identify Fritillaria cirrhosa D. Don., Fritillaria thunbergii Miq. and Fritillaria thunbergii Miq. var. chekiangensis Hsiao et K. C. Hsia. with FTIR.Methods Their IR spectra were obtained by direct FTIR.Results The infrared spectra of F. cirrhosa, F. thunbergii, F. thunbergii var. chekiangensis were different.Conclusion F. cirrhosa, F. thunbergii, and F. thunbergii var. chekiangensis were identified by FTIR directly, fastly and accurately.

Objective To analyze the nutrient constituents of the gonad from Hemicentrotus pulcherrimus.The results could provide a theoretical basis for the development of the H. pulcherrimus.Methods The contents of the water,ash and protein were determined by the national standard methods,total sugar by phenol-sulfuric acid method,rude fat by soxhlet's method,fatty acids by gas chromatography-mass chromatography,and inorganic elements by atomic absorption spectroscopy method.Results The contents of water,ash,rude fat, protein and total sugar were 64.20%,12.70%,2.34%,12.25%and 5.59%,respectively. The contents of arachidonic acid and EPA were higher in fatty acids.Inorganic elements, such as Ca,Mg,Fe were also in higher level.Conclusion The gonad of H.pulcherrimus was useful in the exploitation of nutrient food.

Objective To study the effect of positive lymph node number on the survival of patients with esophageal carcinoma. Methods From July 1995 to July 2005, a total of 11,447 resected lymph nodes were obtained from 1140 patients who underwent curative resection of the primary tumor with systematic lymphadenectomy at Shanxi cancer hospital. The survivals were analysed by life tables and Kaplan-Meier methods, the related factors of lymph node metastasis were assessed by Chi-square test. Results The number of positive lymph nodes was negatively related to survival rates of esophageal carcinoma. According to the number of lymph nodes resected (≥8 nodes versus <8 nodes), there was significant difference in metastatic lymph node ratio. Conclusion The number of positive lymph node can reflect the prognosis of patients better. The authors suggest that the modification of the tumor-lymphnode-metastasis (TNM) staging classification (TNM) to include the number of positive lymph nodes in the N1 category.

Objective:To investigate the expression of miRNA-372-3p (miR-372-3p) in gastric cancer tissues and cells and the regulation of RAB22A protein as well as its effect of miR-372-3p on the biological function of gastric cancer cells.Methods:The expression levels of miR-372-3p in cancer tissues of 70 patients clinically diagnosed with gastric cancer and the pericarcinomatous tissues were detected by using real-time quantitative polymerase chain reaction (RT-PCR) from Shanxi Provincial Cancer Hospital between December 2018 and December 2019. miR-372-3p inhibitor was transfected in gastric cancer MGC-803 and SGC-7901 cells, and miR-372-3p NC (empty vector) was used as the control. The colony formation, proliferation and apoptosis of gastric cancer cells were detected by using the clone formation assay, CCK-8 method and flow cytometry, respectively. Dual-luciferase reporter gene assay was used to detect the expression correlation between miR-372-3p and RAB22A of MGC-803 cells. miRNA-21 (miR-21) was treated as the negative control, and Western blot was used to detect the expression of protein RAB22A in MGC-803 and SGC-7901 cells when miRNA-21 (miR-21) was treated as the negative control.Results:RT-PCR results showed that the mRNA relative expression level of miR-372-3p was up-regulated in gastric cancer tissues compared to their adjacent normal cancer tissues, and the difference was statistically significant (0.51±0.37 vs. 0.77±0.48, t = 1.98, P = 0.005). There were statistically significant differences in mRNA expression level of miR-372-3p in gastric cancer tissues with different tumor diameter and pathological grade (all P < 0.05). The assay in vitro showed that the low expression of miR-372-3p could inhibit the clone formation of MGC-803 and SGC-7901 cells [(211±4) vs. (410±5), t = 2.78, P = 0.001; (244±8) vs. (423±7), t = 2.76, P = 0.001], cell proliferation activity (absorbance value of MGC-803 cells for 48 h: 0.39±0.06 vs. 0.57±0.03, t = 3.18, P = 0.01; absorbance value of MGC-803 cells for 72 h: 0.50±0.05 vs. 0.81±0.06, t = 2.78, P < 0.01; absorbance value of SGC-7901 cells for 72 h: 0.50±0.09 vs. 0.79±0.09, t = 2.77, P = 0.01) and increase the early apoptosis rate of MGC-803 cells [(25.19±0.26) vs. (20.02±0.04), t = 4.30, P < 0.05]. Dual-luciferase reporter gene assay found that compared with the cotransfection of miR-372-3p NC and RAB22A wild type gene, the luciferase activity of MGC-803 cells was decreased after the cotransfection of miR-372-3p inhibitor and RAB22A wild type gene, and the difference was statistically significant [(1.00±0.04) vs. (0.53±0.06), t = 3.18, P = 0.01]. Western blot results showed that knockdown of miR-372-3p could inhibit the expressions of MGC-803, SGC-7901 cells and RAB22A protein. Conclusion:The expression of miR-372-3p in gastric cancer tissues is up-regulated, and miR-372-3p can promote the expression of RAB22A and regulate the occurrence and development of gastric cancer. It may be a potential therapeutic target.

Objective:To investigate the expression of miRNA-522-3p (miR-522-3p) in gastric cancer tissues/cells and its regulation of RSU1 expression and to analyze the effect of miR-522-3p on biological function of gastric cancer cells in vitro.Methods:miR-522-3p relative expression levels in tumor tissues and adjacent tissues of 50 patients clinically diagnosed as gastric cancer in Shanxi Bethune Hospital from May 2019 to June 2019 were detected by using real-time quantitative polymerase chain reaction (qRT-PCR). MGC-803 cells and BGC-823 cells in gastric cancer were divided into miR-522-3p inhibitor group (transfected with miR-522-3p inhibitor) and empty vector group (transfected with empty vector). The cell proliferation was detected by using CCK-8 assay, cell scratch assay was used to detect the scratch healing ability of cells, and flow cytometry was used to detect the apoptosis. The target correlation between miR-522-3p and RSU1 was detected by using double luciferase reporter gene assay, the change of RSU1 protein was detected by using Western blot.Results:qRT-PCR showed that compared with adjacent cancer tissues, the relative expression level of miR-522-3p was up-regulated, and the difference was statistically significant (0.84±0.31 vs. 0.48±0.22, t = 2.93, P < 0.05). There were no statistically significant differences in the relative expression level of miR-522-3p in gastric cancer tissues with different tumor diameter and pathological grade (all P < 0.05). In vitro experimental assay showed that the cell proliferation rates of MGC-803 and BGC-823 cells in miR-522-3p inhibitor group at 48 h and 72 h were decreased (all P < 0.05). Furthermore, MGC-803 and BGC-823 tranfected with miR-522-3p inhibitor could effectively inhibit the scratch healing ability of MGC-803 and BGC-823 cells. Dual-luciferase reporter gene assay verified that miR-522-3p targeted to RSU1 3'UTR and affected its fluorescence activity. Western blot results showed that miR-522-3p could promote RSU1 protein expression. Conclusions:miR-522-3p is involved in the progression of gastric cancer probably via the regulation of RSU1 expression, and it may be a potential therapeutic target.