AIM: To investigate the efficacy and safety of liduofen in the treatment of patients with osteoarthritis of the knee joint. METHODS: A randomized, double-blind, parallel-controlled, multi-centre study was adapted to compare the efficacy and safety between liduofen and Olfen-75 in 136 patients. Patients were randomly assigned into two groups: liduofen group which was received 75 mg (diclofenac) Liduofen injection (n=69) once a day for seven days and Olfen group which was received 75 mg (diclofenac) Olfen-75 injection (n=67) once a day for seven days. RESULTS: After the treatment, similar improvements for rest pain, pain on exercise, swelling of joint, pressing pain of joint, knee joint bend extend function measured using a 10 cm visual analogue scale was performed in two groups. In Olfen-75 group, the rate of efficacy was 62.69% in the 3rd day, 95.76% in the 5th day and 97.88% in the 7th day. While in liduofen group, the rate of efficacy was 57.97% in the 3rd day, 93.87% in the 5th day and 96.94% in the 7th day. In liduofen and Olfen-75 groups, the progressive rates were 56.72% and 55.07%, respectively. The total improvement rates were 89.85% and 92.54% respectively (P＞0.05), and the rates of side effects were 0% and 1.49%, respectively (P＞0.05). CONCLUSION: Liduofen is an effective and safty drug inthe treatment of patients with osteoarthritis of the knee joint. There is no significant difference on efficacy and safety between the two groups using liduofen and Olfen-75.

Objective To investigate the effects of new compound traditional Chinese medicine prepatations Banxiao capsule on the mobilization of endothelial progenitor cells ( EPCs) .Methods After EPCs from mouse bone marrow treated with variable dose of Banxiao capsule, we detected the proliferation by CCK-8 assay, quantified the number of CD34/Flk-1 double positive cells by flow cytometric analysis, evaluated the migratory function of EPCs by transwell chamber assay, and studied the protein level of Bcl-2/Bax by Western blot analysis.Results Compared with untreated group, the proliferation potential of EPCs in Banxiao low dose (20μg/ml) and high dose (200μg/ml) group was increased(P<0.05), the number of CD34/Flk-1 double positive cells were increased (P<0.05), migrative activity of EPCs was increased (P<0.05),the level of Bcl-2 protein were increased, and the level of Bax protein were decreased.Con-clusion Banxiao capsule can enhance proliferation of EPCs through up-regulating of expression of Bcl-2/Bax.

0.05 ). CONCLUSION: Liduofen is an effective and safty drug inthe treatment of patients with osteoarthritis of the knee joint. There is no significant difference on efficacy and safety between the two groups using liduofen and Olfen 75.

Medication is still the main means for treating liver cirrhosis. However, micro-circulation obstacle in the liver and dynamic abnormality of portal vein flow suggest that liver protecting drugs via peripheral veins can hardly give full requirement for their effect. The authors reviewed a new method of percutaneous transhepatic portal vein implantation of port-catheter system for infusion of drugs in the treatment of liver cirrhosis, with theoretical basis and the outlook of application.

Objective To investigate the change of anxiety in patients undergoing coronary angiography. Methods The Spielberger State-Trait Anxiety Inventory was carried out in hundred and forty five patients, 104 men, 41 women, mean age (63?9) years, before and after the coronary angiography procedure respectively. Results The state anxiety score after the procedure was significantly decreased from 35.8?10.2 to 31.7?9.9(P

OBJECTIVE To introduce experiences of cosmetologic reparation after malignant nasal cutaneous tumor resections.METHODS Cutaneous flap was chosen to reconstruct 37 cases of defects after nasal malignant tumor resection according to their area,shape and site,in which basal cell carcinoma were 25 cases,squamous cell carcinoma were 12 cases,respectively.Nasal cutaneous defects rangeed from 0.8 cm?0.8 cm to 5.0 cm?4.0 cm.The flap came from diamond flap 9 cases,from nasolabial island flap 15 cases,from subcutaneous pedicel flap 13 cases,by which to supply directly pulling saturation of flap area. RESULTS All cutaneous flaps of 31 cases survived with primary healing;nasal appearances were good and no malignant tumor recurred after 1-18 months follow up.CONCLUSION Employ local cutaneous flap with color and texture matched to cosmetologic reconstruction of defects after nasal malignant tumor resections are capable of reach satisfied clinical effect.

Objective:To investigate the relationship of the expression of VEGF-C and the metastasis of the lymphoid nodes in laryngeal cancer.Methods:Using immunohistochemistry method, the expression of VEGF-C in the tumor tissues and the density of lymphatic in adjacent tissue of laryngeal carcinoma were detected.Results:The expression of VEGF-C in laryngeal carcinoma was higher than in vocal cord polyp and in lymphoid node-positive was higher than in node-negative. Density of lymphatic capillary in adjacent tissue of laryngeal carcinoma was much higher than in normal laryngeal tissue and in lymphoid node-positive was higher than in node-negative.Conclusion:VEGF-C protein expression is close to lymphatic metastasis.

BACKGROUND:Recent studies have found that long non-coding RNAs (lncRNAs) can regulate stem cel proliferation and differentiation. But it is unclear that how lncRNA NR_033474 regulate stem cel proliferation and cel cycle. OBJECTIVE:To investigate the effect of lncRNA NR_033474 on the proliferation and cel cycle regulation in C3H10T1/2 mesenchymal stem cel s after the NR_033474 overexpressed by lentivirus, and to study the possible regulation mechanism of NR_033474 on mesenchymal stem cel s. METHODS:LncRNA NR_033474 was cloned into a lentivirus vector. Lentivirus particles were infected into C3H10T1/2 cel s to upregulate the expression of NR_033474. The NR_033474 expression level was detected by real-time PCR. Compared with the empty lentivirus vector, the proliferation of C3H10T1/2 cel s which overexpressed NR_033474 was detected by cel counting assay and cel cycle was detected using flow cytometry. The expression of cel cycle-associated proteins such as CDK1, Cyclin B1, Cyclin D1 and P53 were detected by western blot assay. RESULTS AND CONCLUSION:Compared with the control group, lncRNA NR_033474 in C3H10T1/2 cel s which overexpressed NR_033474 was increased by about 100 times (P<0.01), and the proliferation of C3H10T1/2 cel s was significantly inhibited after NR_033474 overexpression by lentivirus (P<0.05). In addition, flow cytometry showed that C3H10T1/2 cel s overexpressing NR_033474 were arrested in G2/M phase compared to the control group. Western blot showed that the expression levels of CDK1 and Cyclin B1 were downregulated, while there were no changes in Cyclin D1 and P53 expression. To conclude, these findings suggest that the NR_033474 overexpression significantly inhibits the cel growth of C3H10T1/2 cel s, at least in part, through induction of cel cycle arrest at G2/M phase.

Objective: To investigate the effects of hyaluronidase (HAase) and hyaluronan (HA) on proliferation of vascular endothelial cells and its mechanism. Methods: The cultured bovine aortic endothelial cells (BAEC) were treated with HAase or HA. Cell proliferation rate was detected by MTT assay. The expression of CD44 and DNA content of the cells were measured by flow cytometry (FCM). Results: HAase (50 ?g/ml) stimulated cell proliferation [(50.10? 1.23)% vs control, P

Objective: To investigate the influence of mental stress on platelet function in patients received coronary angiography(CAG). Methods: With flow cytometry the expressions of platelet membrane glycoproteinⅡb Ⅲa complex ? subunit(CD41) and P selectin(CD62p) were measured in 24 CAG patients before,immediately after and 10 min after mental stress, respectively. Results: Compared with that before mental stress, the positive rate of CD41 [from(74.15?22.24)% to (87.41? 9.24)%, P