ObjectiveTo investigate the therapeutic effect and the level of C-reactive protein of Edaravone on acute cerebral infarction after thrombolytic therapy in urokinase,and to discuss edaravone on brain tissue protective effect of thrombolytic. Methods41 patients with cerebral infarction were,divided into 2 groups randomly:treatment group(19cases) and control group(22 cases). Control group received thrombolytic therapy,and treatment group was treated with Edaravone at a dose of 30mg,twice a day,for 14 days. Clinical outcomes and neurological deficit score were evaluated before and after treatment. At the same time, serum CRP levels in patients were determend by immune turbidimetry. ResultsThe treatment group whose neurological deficit score better than the control group ( P ＜ 0.05),the total effective rate was significantly higher( P ＜ 0.01 ) ,compared with the control group, the treatment group CRP levels were significantly lower ( P ＜ 0. 05 ). ConclusionEdaravone on acute cerebral infarction in patients after thrombolysis can significantly improve the clinical efficacy, reduce serum CRP levels, recovery and prognosis of cerebral infarction is important, Edaravone can significantly improve neurological function in patients with acute cerebral infarction,protects against cerebral ischemia after thrombolysis in metabolic poisons further damage.

Objective:To study the 3D accuracy of 4 impression materials.Methods:A metal mould with a custom tray was prepared for the measurement.Impression samples of 2 alginate and 2 rubber impression materials were prepared in the metal mould(n =3)re-spectively.The length of three tagged lines,along X,Y,Z axis directions of the samples was measured with a 3D optical scanner.Re-sults:120 min after sampling the variable rates of the length along X,Y,Z axis of Zhermark alginate samples were 3.615%,3.037%and 2.836%,those of CAVEX alginate samples were 2.836%,3.358% and 4.276% respectively;8 h after sampling the variable rates of Impregum Penta polyether rubber samples were 0.039%,0.071% and 0.057%,those of ExpressXT Penta H addition-curing silicone rubber samples were 0.033%,0.088% and 0.084% respectively.Conclusion:The variable rates of the 4 impression materi-als are in accordance with ISO1563∶1990.The variable rate of alginate impression material is greater than that of rubber.

Objective　To investigate the effect of rapid fluid replacement on hemorheology in delayed resuscitation after burn. Methods　A total of 32 dogs inflicted with 40%TBSA full thickness scalding were randomly divided into 4 groups: scald control group(C group), delayed Gelofusion even replacement group (GE group), rapid fluid replacement group (GR group), and delayed plasma rapid fluid replacement group (PR group). The femoral arterial pressure, viscosity of blood and plasma, packed cell volume and aggregation of RBC were detected at the intervals of before and 2, 6, 8, 12, 24, 36 and 48 hours after scalding. Results　The viscosity of blood markedly increased at 2 hours after scalding, and the hemorheology parameters decreased after fluid resuscitation. The hemorheologic parameters were obviously lower in GR group than in GE group at 2 hours after rapid resuscitation, the viscosity of blood and RBC aggregation in GR group were obviously lower than those in PR group. Conclusion　Under the condition of delayed resuscitation after burn, rapid fluid replacement can quickly decreased the state of blood high viscosity and may play a role in improving microcirculation and treating burn shock.

Objective To construct the small interfering RNA (siRNA) eukaryotic expression vector specific to human MGMT gene(pRNATin-H1.2/Neo MGMT siRNA) to observe its silencing effect on MGMT gene in vitro.Methods The pRNATin-H1.2/Neo MGMT siRNA expression vector was constructed by gene recombination,then transfected into the cultured HelaS3 cells.Inhibitory effect of siRNAs was detected by semi-quantitative RT-PCR.Results The pRNATin-H1.2/Neo MGMT siRNA expression vector was successfully constructed.Cells transfected with pRNATin-H1.2/Neo MGMT siRNA could obviously inhibit the expression level of MGMT gene.Conclusion The pRNATin-H1.2/Neo MGMT siRNA expression vector could inhibit the MGMT gene expression.

Background Package and tissue patch implantation are common methods for repair of filtering bleb leaking after anti-glaucoma surgery.But the scarring or re-leakage of filtering bleb probably occur again.Objective This study was to investigate the repair effect of acellular dermal matrix (ADM) on filtering bleb leaking in rabbit model and compare the effectiveness among ADM,amniotic membrane and conjunctival overlap.Methods Trabeculectomy was performed on 48 eyes of 24 New Zealand rabbits,and models of filtering bleb leaking were established.The models were randomized into ADM group,amniotic membrane group and conjunctival covering group based on randomized number table.ADM patches with 4 mm×4 mm were implanted across lamellar cornea and sclera at a bridge in the ADM group,and the same size of amniotic membranes were used in the amniotic membrane group,and conjunctiva was sutured to limbus in the conjunctiva overlap group.The intraocular pressure (IOP) was measured before surgery and 1 day,1 week,1 month,3 months and 6 months after surgery.The biocompatibility of materials above was assessed under the slit lamp microscope,and the status of filtering bleb was evaluated and compared with anterior segment optical coherent tomography (AS-OCT) 1 day,1 week,1 month,3 months and 6 months after surgery.Results Before surgery and 1 day,1 week,1 month,3 months and 6 months after surgery,the IOP was (26.9±4.3),(16.6±5.1),(22.1 ±6.2),(18.3±6.5),(22.7±2.5),(23.4±1.4) mmHg in the AMD group,(29.9±5.4),(14.9 ± 6.4),(21.6 ± 7.8),(26.3 ± 4.1),(26.0 ± 4.2) and (23.0 ± 5.3) mmHg in the amniotic membrane group,and (28.7 ±4.3),(15.7 ±7.0),(22.0±6.3),(28.2±4.1),(24.7 ±4.1),(23.0±2.7) mmHg in the conjunctival overlap group,showing significant differences among different groups and various time points (Fgroup =8.419,P=0.011 ;Ftme =15.543,P=0.000).The IOPs were significantly lower from 1 day through 3 months after operation than those before operation in the AMD group (P =0.000,0.000,0.006,0.045) ; while the IOPs were reduced only from 1 day through 1 week after operation in comparison with before operation in the amniotic membrane group and the conjunctival overlap group (P =0.000,0.001).One month after surgery,the IOPs were significantly declined in the ADM group compared with the amniotic membrane group and the conjunctival overlap group (P =0.001,0.000).The grafts were clear under the slit lamp microscope and exhibited the valid filtering bleb until 3 months after operation under the AS-OCT in the ADM group.However,the valid filtering bleb remained only 1 month after surgery in the amniotic membrane group and the conjunctival overlap group.Neovascularization on the filtering bleb was found 3 months in the AMD group but 1 month in the amniotic membrane group and the conjunctival overlap group.Conclusions Compared with amniotic membrane and conjunctival tissue,ADM patch for the repair of filtering bleb leakage can increase the survival duration of filtering bleb and remain lower IOP.

Objective To study the expression and clinical significance of ARHGAP4 in colorectal cancer Method Real?time PCR,Western blot and immunocytochemistry were used to detect the expression of ARHGAP4 in colorectal cancer tissues and cell lines ,and the correlation between its expression and clinical features of patients was analyzed Results ARHGAP4 is overexpressed in colorectal cancer tissues and cell lines and its overexpression is correlated with T stage, N stage, clinical stage, and metastasis. Conclusion ARHGAP4 may promote the progression of colorectal cancer ,and have the potential to be a novel prognosis marker.

Objective:To collect the imaging data and related materials of the patients with in-stent restenosis (ISR)after coronary artery stent operation with intravascular ultrasound (IVUS),and to analyze the risk factors of ISR,and to propose the reasonable intervention strategies.Methods:Fifty patients with ISR were divided into ISR ≤ 50% drug group (n = 14 )and ISR > 50% drug group (n = 36),including drug-coated balloon therapy group (n=16)and stent treatment group (n=20);IVUS virtual organization technology was used to compare the plaque area,location,tissue composition,thrombus and other factors of the patients in various groups after treatment;the data changes after 6 months of follow-up were analyzed.Results:The IVUS results showed the plaque areas and plaque loads of the ISR patients treated with intervention were significantly reduced compared with before operation (P <0.05);the plaque compression degree of the patients in drug-coated balloon therapy group was lower than that in stent treatment group (P <0.05),but the differences were not found between drug-coated balloon therapy group and stent treatment group in fibrous tissue components and calcified tissue proportion (P >0.05).Conclusion:The ISR rate is higher in the patients with high degree of fiber components,plaque composition heterogeneity and distribution of diffuse tortuous and calcified lesions.ISR has no significant correlation with the plaque wall thickness and lipid content and plaque instability and necrotic tissue proportion.

Objective:To investigate the understanding and application of the knowledge of premature infants in pediatricians participating in the Second Chinese Preterm Infant Academic Conference through questionnaires, and to carry out targeted continuing education for pediatricians in order to better guide the clinical diagnosis and treatment of grassroots pediatricians.Methods:The research was conducted in the form of a questionnaire survey.The survey objects were pediatricians participating in the " Second Chinese Preterm Infant Academic Conference" , and the results of the questionnaire were summarized and analyzed.The questionnaires were designed based on personal education background, employment time, current status of neonatal specialty in the hospital where they were, and knowledge of premature infant nutrition.Results:A total of 554 valid questionnaires were received.Among the pediatricians surveyed, 37.2% had a master's degree or higher; 344(62.1%) pediatricians came from Grade Ⅲ class A hospitals.According to the geographical distribution, age, education, working years, hospital level, hospital nature, annual delivery of obstetrics in hospitals, the number of beds in neonatal specialty wards, and the number of beds in NICU, the respondents were grouped, and the current status of premature births in China and the situation of using the growth curve(Fenton2013) of premature infants in work were compared.It was found that the proportion of those who used the Fenton curve of the 2013 version in Grade Ⅲ class A hospitals was higher than that in Grade Ⅲ class B hospitals and Grade Ⅱ hospitals(χ 2=47.663, P<0.05). The ratio was higher among women and children's health centers and general hospitals(χ 2=8.072, P<0.05). Conclusion:The academic annual conference for the purpose of continuing education, set up a pre-conference questionnaire for the participants during the preparation period, and set the content of the annual conference according to the needs of the target population, which can better solve the clinical problems of primary doctors and promote the improvement of primary doctors' diagnosis and treatment.

Objective:To construct and validate a prognostic model for bladder cancer based on single-cell RNA sequencing (scRNA-seq) bioinformatics analysis of prognosis-related differential expression genes.Methods:The bladder cancer scRNA-seq datasets like GSE135337 and GSE129845 were downloaded from Gene Expression Omnibus (GEO) database, and the data were updated in 2022 and 2019; the expression profile and the survival data of 165 bladder cancer samples in the conventional transcriptome dataset GSE13507 (the data were updated in 2020) were downloaded. Expression profile data of 414 bladder cancer samples and 19 paracancerous samples and clinical information of 405 bladder cancer patients were downloaded from The Cancer Genome Atlas (TCGA) database. R 4.1.2 software was applied in the quality control and downscaling clustering of 10 bladder cancer single-cell samples selected from the GEO database and the cell annotation was made. The cellular communication of single cell data in the GEO database was analyzed by using CellChat. Univariate Cox proportion hazards model was used to analyze the differential expression genes related to prognosis of bladder cancer. The prognostic risk model was constructed by using LASSO-Cox regression analysis and the risk score was calculated. According to the median risk score, the bladder cancer patients in TCGA database were treated as the training set and all patients were divided into high‐risk group and low‐risk group. GSE13507 dataset in GEO database was used as the validation set, and the Kaplan-Meier method was used to compare the overall survival of the two groups in the TCGA training set and the GEO validation set; the time-dependent receiver operating characteristic (ROC) curves were used to evaluate the predictive efficacy of the prognostic risk model. R 4.1.2 software was used to construct the nomogram for predicting the 1-, 3- and 5-year overall survival rates of patients. Correlation analysis of risk score and clinical characteristics of bladder cancer patients in TCGA dataset was performed. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and gene set enrichment analysis (GSEA) were performed.Results:In GSE135337 and GSE129845 datasets, a total of 50 263 cells were obtained after the filtration of quality control, including 43 519 uroepithelial cells. More interaction between uroepithelial cells and fibroblast could be found in the microenvironment of bladder cancer. Uroepithelial cells sent signals mainly through the midkine signaling pathway. Finally, 9 prognosis-related differential expression genes (SPINK1, FN1, EFEMP1, ELN, PCOLCE2, TUBA1A, COL14A1, TCF4, and TM4SF1) were screened and the prognostic risk model was constructed. The risk score was calculated as -0.019×SPINK1+0.028×FN1+0.025×EFEMP1+0.023×ELN+0.098×PCOLCE2+0.004×TUBA1A+0.047×COL14A1+ 0.004×TCF4+0.096×TM4SF1. Based on the median risk score (1.350), the overall survival of the high-risk group (≥1.350) was worse than that of the low-risk group (<1.350) in the training set and the valiation set. ROC curve analysis showed that the area under the curve (AUC) of 1-, 3- and 5-year overall survival rates in the training set and the validation set were larger than 0.65. Based on the age, staging and prognostic model risk score, a nomogram was constructed to predict the 1-, 3- and 5-year overall survival rates of patients, and its calibration curve was close to the ideal curve. The risk scores were elevated in patients aged more than 60 years old, M 1 in M staging, N 1, N 2 and N 3 in N staging, and stage Ⅲ and Ⅳ in TNM staging, and the differences were statistically significant (all P < 0.05) . Enrichment analysis showed that several significantly-enriched genes were associated with functions and pathways such as humoral immune response, granulocyte chemotaxis, cytokine-cytokine receptor interactions, and B-cell-mediated immunity. Conclusions:The stable prognostic prediction model for bladder cancer constructedbased on scRNA-seq data can provide a reference for clinical assessment of patients' prognosis.

Based on the Pharmaceutical Comprehensive Knowledge and Skills Outline in the year of 2003, 2007, 2011 and 2015,the changes and the trend in the chapters and contents of Pharmaceutical Comprehensive Knowledge and Skills were analyzed and discussed,the meaning of versions was elaborated,and the shortcomings were discussed as well. The catalog of the chapters and contents conformed to the economic and social development in China,which is moving toward the direction of clinical pharmacy service.