Abstract: To explore the mechanism of the intestinal microecology regulation by polysaccharide prebiotics, ELISA, histopathologic analysis, immunohistochemical analysis, 16S rRNA high-throughput sequencing, and gas chromatography-mass spectrometry were applied to investigate the effects of fermented polysaccharides on changes in the intestinal microbiota and short-chain fatty acids (SCFAs) in mice with dextran sulfate sodium (DSS)-induced colitis model and their relationship with the level of intestinal inflammation and barrier protein expression. It was found that fermented Lycium barbarum polysaccharides (FLBP) significantly reduced intestinal inflammation level, improved colonic tissue structure, up-regulated the expression of tight junction proteins Claudin-1 and ZO-1, and significantly increased the content of intestinal SCFAs in mice. Gut bacteria analyses showed that FLBP enriched intestinal Dubosiella and Akkermansia in mice and decreased the abundance of Turicibacter, Faecalibaculum, and Escherichia-Shigella. Results showed that remodeled Dubosiella activated by FLBP played a dominant role in ameliorating colitis by significantly increasing SCFAs content, improving intestinal barrier and reducing intestinal inflammation. The study aimed to provide a safer and better option for the amelioration of colitis and to provide a theoretical basis for the development of functional foods with FLBP.

Objective : To explore the influence and mechanism of different types of proteins on the corrosion resistance of alloy to provide a reference for the safe application and surface modification of nickel-titanium (Ni-Ti) and stainless steel bow wires in the clinic. Methods: The effects of fibrinogen, IgG and mucin on the electrochemical corrosion resistance of Ni-Ti and stainless steel arch wires were tested by the potentiodynamic polarization method, and the repair ability of passive films on surfaces treated with the three proteins were tested by the cyclic polarization method. Inductively coupled plasma optical emission spectrometry (ICP-OES) was used to determine the types of corrosion products, and the surface morphology after corrosion was analyzed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results : The addition of fibrinogen, IgG or mucin to an alloy has different effects on its corrosion resistance. Adding protein can reduce the corrosion resistance of stainless steel alloys and slow the corrosion process of Ni-Ti alloys. The addition of mucin can improve the corrosion resistance of Ni-Ti alloy and the repair ability of passive film. Compared with mucin and IgG, fibrinogen can reduce the pitting resistance of Ni-Ti and stainless steel alloys. Conclusion Different types of proteins interact differently with the arch wire, form different deposition morphologies on the surface, and participate differently in the corrosion process of the alloy.

To investigate the experience and efficacy of endoscopic thyroidectomy for papillary thyroid microcarcinoma (PTMC) through total areola approach.
 Methods: A total of 117 PTMC patients, who were diagnosed pathologically in Minimally Invasive Surgical Center, Second Xiangya Hospital, Central South University from June 2016 to December 2017, were divided into a endoscopic surgery group (n=72) and an open surgery group (n=45). The number of dissected central lymph nodes, blood loss, amount of drainage, occurrence of postoperative complication and recurrence were collected and compared.
 Results: Compared with the open surgery group, the blood loss was less and the operative time was longer in the endoscopic surgery group (P<0.05). There were no significant differences between the 2 groups in the number of dissected central lymph nodes, amount of drainage and occurrence of postoperative complication (all P>0.05). The mean follow-up time was more than 20 months, and there was no recurrence in the 2 groups. 
 Conclusion: Endoscopic thyroidectomy with central compartment neck dissection through total areola approach is safe and feasible in patients with PTMC. It has many advantages, such as no scar on neck, less blood loss, shorter hospital stay and more acceptable to young patients.
Carcinoma, Papillary ; surgery ; Endoscopy ; Humans ; Nipples ; Thyroid Neoplasms ; surgery ; Thyroidectomy