1.Cortical reorganization for calculation in patients with left temporal lobe epilepsy
Zhongming LI ; Ziqian CHEN ; Zhimin WANG ; Yuntao WANG
Chinese Journal of Physical Medicine and Rehabilitation 2015;37(2):138-141
Objective To observe the brain regions activated during calculation in patients with left temporal lobe epilepsy (TLE) and in normal subjects.Methods Functional magnetic resonance imaging (fMRI) was applied.Sixteen right-handed persons with left TLE were selected as the left TLE group,and sixteen healthy volunteers were recruited as a control group.The fMRI data was collected as each subject performed simple additions,subtractions and abdication subtractions.Statistical parametric mapping was used to compare the activated brain regions between the two groups.Results The error rate in the calculations was significantly higher in the left TLE group,and their average reaction time was significantly longer.There were aslo significant differences between the two groups in terms of brain activation patterns.Compared with the control group,the left TLE group exhibited hypo-activity in regions such as the left paracentral lobule,the posterior central gyrus,the bilateral inferior parietal lobule,the left angular gyrus,the bilateral supramarginal gyrus,the left middle frontal gyrus,the left superior gyrus,and also in the bilateral posterior cingulate,insular lobule,superior and inferior temporal gyrus,right hippocampus,parahippocampal gyrus,thalamus and cerebellum.The TLE subjects exhibited hyper-activity in the bilateral superior parietal lobule,the bilateral anterior cingulate,as well as in the right,middle and inferior frontal gyrus.Conclusion Our results support a significant functional reorganization of calculation-related neuronal networks within and between the hemispheres in TLE patients.The frontal and parietal lobes may play a compensatory role in the reorganization of the calculation function.Task-related fMRI technology can provide useful information for non-invasive assessment of mathematical computing and cognitive function in TLE patients.
2.A Comparison of Antidotal Potency of DMAP & Am NO_2 against Inhaled HCN Intoxication in Dogs
Ziqian OUYANG ; Xiangde WEI ; Jiafeng WANG ; Xinzong LIN ; Shaohong MOU ;
Journal of Third Military Medical University 1983;0(04):-
The toxicity of HCN inhaled via the respiratory tract in dogs and the therapeutic effects of DMAP and AmNO2 for such form of HCN intoxication were studied. The LD50 of HCN when inhaled was 850.4?80.4?g/kg.When the dosage of 2?LD50 of HCN was given to the experimental animals, the signs of intoxication developed rapidly. Uneasiness, struggling, strident barking, and respiratory excitation were observed several seconds after the exposure. Subsequently there was spasm of the extensors and rigidity of the extremities. Finally general inhibition and paralysis ensued. Respiration and heart beat stopped 6 and 7 minutes after exposure. If DMAP (3.25 mg/kg intramuscularly) or AmNO2 (2 ampules through inhalation) was administered 15 seconds after exposure, the survival rate of the animals was 90% or 100% respectively.When the dosage of 4?LD50 of HCN was inhaled and the two drugs were administered 45 seconds after exposure, the survival rate of the animals treated with DMAP remained as high as 90% (9/10) .But the survival rate of those treated with two ampules of AmNO2 or even three ampules reduced to 20% or 40% respectively.The amount of HCN inhaled during respiratory intoxication, the problems of early diagnosis and the administration of DMAP at the site of accident were analyzed and discussed.
3.Research on reform of teaching Chinese for international clinical medical undergraduates in Chongqing Medical University
Feng LIU ; Ziqian YU ; Yaxin TAN ; Yan WANG
Chinese Journal of Medical Education Research 2013;(7):702-704
To adapt to the new training program and requirement of MOE , a reform in Chinese curricula, textbook selection and editing, and teaching evaluation method was carried out to strengthen medical Chinese teaching and improve the students' overall Chinese proficiency. Detailed measures in-cluded adjusting the class hour, formulating Chinese reading and writing syllabus, Chinese listening and speaking syllabus and graded vocabulary, evaluating the effect through Chinese proficiency test, learning evaluation and file cover evaluation. After the reform, attendance rate was improved obviously, plagiarism rate was declined obviously and students were highly praised by teacher during the internship and practice.
4.Experimental studies of formulated Chinese medicinal herbs as a vascular embolizing agent
Junjie SHEN ; Ziqian CHEN ; Shudong WANG ; Xiaogang ZHENG ; Jianmin CAO
Journal of Medical Postgraduates 2003;0(10):-
Objective: To study the efficacy,safety and relative characteristics of formulated Chinese medicinal herbs as a vascular embolizing agent. Methods: We prepared a mixture as a vascular embolizing agent from several Chinese medicinal herbs,they served as anticarcinogen and/or coagulant according to the Chinese Pharmacopeia.We studied the characteristics of the mixture through embolizing the hepatic artery in eight pigs with it. Results: The mixture was prepared as a suspension.It was easily injected through catheters and showed good histocompatibility and hemo-compatibility without pyrogenetic response and toxicity.The mixture mainly embolized peripheral arteries,maintaining occlusion for 5 weeks and without formation of collateral circulation.We found injuries of normal hepatic tissues as slight hepatocyte hydrops and focal endochyloma necrosis through optical and electronic microscope. Conclusion: This suspended mixture of Chinese medicinal herbs is safe and effective as a potential peripheral embolizing agent.
5.Advance and application of interventional embolic agents
Junjie SHEN ; Ziqian CHEN ; Jianmin CAO ; Shudong WANG ;
Journal of Medical Postgraduates 2003;0(05):-
The development and application of embolic agents is a very important step to vascular embolotherapy and many studies about it have been done at home and abroad.We must know their characteristics very well and choose situable embolic agents and interventional methods.Besides we also need to manufacture even ideal embolic agents.This text reviews the advance and application of many kinds embolic agents.
6.Evaluation of Nasopharyngeal Carcinoma Distant Interval Metastasis and Diagnostic Efficacy by ~(18)F-FDG PET/CT
Tianran LI ; Jiahe TIAN ; Chunlei ZHAO ; Ziqian CHEN ; Hui WANG
Journal of Medical Research 2006;0(11):-
Objective To summarize that the distant interval metastasis of nasopharyngeal carcinoma(NPC) patients who have been examined by 18F-FDG PET/CT and analysis the diagnostic efficacy of doctors using PET/CT device.Methods 75 patients were divided into three groups including primary patients without any treatment and patients in treating and patients after radiotherapied.The distant interval metastasis sits,incidence and their difference were analyzed.We adopt five grades to diagnose distant interval metastasis of NPC.And we choose the difference grade as cut off point to draw ROC curve and then decide the best diagnostic cut off point.Result There are 22patients in the first group and 2patients in the second group and 51patients in the third group in all 75paients.The incidence of distant interval metastasis in the first group is 59.1% and third 68.6% and there are statistical significance between groups(P=0.0001).The most frequent sites of distant interval metastasis is the distant interval lymph nodes(38.67%) and the second is skeleton(36%) and the third is liver(25.33%).ROC curve indicate that third one is the best cut off point because of doctors using PET/CT device can acquire the optimal sensitivity(86.36%)and specificity(88.24%) and accuracy(76.8%).Conclusion The incidence of distant interval metastasis is higher and the most frequent part is distant interval lymph nodes.And the doctors using PET/CT device diagnose the distant interval metastasis of NPC efficacy is higher.
7.Investigation of aquaporin-1 expression and diffusion weighted imaging with multiple b values in adrenal gland in a rat model of diabetes mellitus
Yu WANG ; Heng ZHANG ; Ruzhi ZHANG ; Ziqian XU ; Lei WANG ; Rongbo LIU ; Fabao GAO
Chinese Journal of Radiology 2017;51(8):616-620
Objective To investigate the character of aquaporin-1(AQP-1)expression in adrenal gland in diabetes mellitus and evaluate adrenal gland damage and function alterations by DWI with multiple b values. Methods Twenty male Sprague-Dawley rats were randomly selected by computer and randomized into 2 groups:untreated controls(n=10)and diabetes(DM)(n=10). Rats in diabetes group were fed with high-sucrose and high-fat diet, controls were fed with common diet. After fed with high-sucrose and high-fat diet for 4 weeks, rats in diabetes group were injected with streptozotocin(STZ). Forty days after diabetes induction with streptozotocin(STZ), MR imaging was performed in a 7.0 T scanner. Venous blood from the tails was collected before MRI scan to measure blood glucose, blood glucose more than 16.7 mmol/L wasregarded as diabetic status. All the rats underwent DWI with 18 b values(0 to 4500 s/mm2). Maps of pure diffusion coefficients(D), pseudo-diffusion coefficients(D*)and ultra-high ADC(ADCuh)were acquired. Rats were sacrificed after MRI scan for adrenal gland histopathology, AQP-1 immunohistochemistry analysis and AQP-1 optical density(OD)measurements. Student t test was used to compare the difference of D*, D, ADCuh and OD of AQP-1 between two groups. Results Eight diabetic animals developed hyperglycemia(two rats died during the modeling process). MRI scan was performed in all of the 18 rats. Signal intensity of D*map, D map and ADCuh map decreased gradually. ADCuh increased significantly in DM animals(0.24 ± 0.06) × 10-3mm2/s compared with control animals(0.18 ± 0.07) × 10-3 mm2/s(P<0.05), whereas there was no significant difference found between the two groups in their respective D*and D values(P>0.05). There was a noticeable increase in the AQP-1 labeling in the adrenal cell membrane and cytoplasm in DM animals compared with control animals. DM rats showed an increased OD value of AQP-1 in adrenal gland compared with the control animals(P<0.05). Conclusions We found significantly higher AQP-1 expression in adrenal gland in DM animals compared with controls. Ultra-high b-Values DWI may work as a useful way for noninvasive evaluation the change of adrenal function in DM.
8.Clincal study of treatment for refractory diabetic wound
Zhenqiang SONG ; Runxiu WANG ; Yuan LIN ; Daen LIU ; Ziqian LIANG ; Liming ZHANG ; Qingwen NONG ; Shuliang LU
Chinese Journal of Trauma 2010;26(8):731-733
Objective To explore the repair method for refractory diabetic wound. Methods A total of 206 patients with refractory diabetic foot ulcers were treated with proper surgical treatments.Results Of all, 106 patients were treated by skin flap (51.5 % ), with one stage wound healing rate of 85.8%; 122 patients were repaired with split-thickness skin graft ( 59.2% ), with survival rate of the graft for 79.5%. Simple toe amputation was made in 34 patients (46 toes). The high level amputation was performed in 56 patients (27.2%). Of all, 132 patients were followed up for 6-18 months, which showed that ulcer recurred in 12 patients (9.1%). Conclusion Timely and effective treatment as well as flap and skin graft repair could reduce high level amputation rate of diabetic foot ulcer and promote the quality of life.
9.Laparoscopic total gastrectomy for gastric cancer
Feng QIAN ; Peiwu YU ; Ziqian WANG ; Bo TANG ; Yan SHI ; Yongliang ZHAO ; Huaxing LUO ; Gang SUN
Chinese Journal of General Surgery 2008;23(4):262-264
Objective To investigate the feasibility,method and result of laparoscopic total gastrectomy for gastric cancer. Methods Clinical data of 63 cases of gastric cancer treated with laparoseopic total gastrectomy were analyzed retrospectively. Results In this study,52 cases underwent laparoseopic radical total gastrectomy and 5 cases did laparoseopic palliative total gastrectomy.The procedure Was hand assisted in tlle first 45 cases for fashoning esophagojejunostomy through a small incision.In six cases the procedure was converted to open surgery.The operative time was(312±35)min,the blood loss was(190±50)ml,the number of lymph nodes dissected Was(32±7).It began to pass flatus(4.0±1.2)days postoperatively.It was(4.5±1.5)days to start oral liquids.Patients were up and about on(4.0±1.5)days postoperatively. Minor postoperative complications occurred in 5 cases. Conclusion Laparoscopic total gastrectomy for gastric cancer is safe,feasible,less traumatic and of fast postoperative recovery.
10.Statistical parametric mapping-based interhemispheric asymmetry analysis of 18F-FDG PET brain imaging in localization of unilateral temporal lobe epileptic foci
Xiaoyang WANG ; Chunlei ZHAO ; Hui LI ; Liyuan FU ; Dandan LIN ; Ziqian CHEN
Chinese Journal of Nuclear Medicine and Molecular Imaging 2017;37(9):538-543
Objective To evaluate the diagnostic value of the SPM-based interhemispheric asymmetry analysis (SIAA) of 18F-FDG PET brain imaging in localization of temporal lobe epileptic foci.Methods A total of 73 patients (42 males,31 females,average age: (27.10±9.78) years) diagnosed as temporal lobe epilepsy (TLE) and 30 healthy volunteers (17 males,13 females,average age: (33.79±5.46) years) from March 2012 to July 2015 were enrolled in this retrospective study.18F-FDG PET brain imaging was performed on all subjects to localize temporal lobe epileptic foci.The results of 18F-FDG-PET were analyzed by SIAA,visual analysis and asymmetry index (AI) analysis.The accuracy of the three methods was evaluated according to the postoperative pathological results and curative effect.χ2 test and partition of χ2 test were used to analyze the data.Results The accuracies of localization with SIAA,visual analysis and AI analysis were 87.67%(64/73),75.34%(55/73) and 69.86%(51/73),respectively (χ2=6.993,P<0.05).The accuracy of SIAA was higher than that of visual analysis and AI analysis (χ2 values: 5.818 and 8.481,both P<0.017),while there was no significant difference between AI analysis and visual analysis (χ2=0.500,P>0.017).Conclusion The SIAA of 18F-FDG PET brain imaging may be a more objective and accurate method in localization of TLE foci.