Aim Toinvestigatingtheinductionof CYPs in hepatocytes or HepG2 cells by triptolide(TP) andthepossiblemechanism.Methods AfterTPtreat-ment,the expression of CYPs in rat primary hepato-cytes or human HepG2 cells was detected by real-time PCR and Western blot assays.Specific inhibitors or gene knockdown method were employed to analyze the possiblemechanism.Results Theexpressionof CYP1A2,2C7,2C11,2C12,2D2,2E1 and 3A1 in rat primary hepatocytes was induced by TP.The fold was 19,2,31,3,21,88 and 34 at 50 nmol·L-1, respectively while at 100 nmol·L-1 it was 20,5,30,23,61,83 and 38,respectively.In HepG2 cells,the expression of human CYP1A1,2B6,2C9,2C19, 2D6,2E1 and 3A4 was also induced by TP.The ac-tivities of nuclear receptor PXR and CAR were inhibi-ted.TP upregulated p53 expression,and the induction of several CYPs caused by TP was blocked when p53 wasinhibited.Conclusions TPinducesCYPsexpres-sion in rat hepatocytes and HepG2 cells.Nuclear re-ceptors may not be involved in TP induced CYPs, while the mechanism may partly attribute to p53.

Objective To construct lentiviral expression plasmids of CYP2C9,CYP2C9 * 8 and CYP2C9 * 27,and to express CYP2C9,CYP2C9 * 8 and CYP2C9 * 27 stably in HEK2C9T cells.Methods CYP2C9 coding region was obtained by reverse transcription PCR from the human liver total RNA,and then cloned into the mammalian expression vector MigR1.CYP2C9 * 8 or CYP2C9 * 27 coding sequences were obtained from MigR1-CYP2C9 by overlapping PCR and then cloned into MigR1.The coding sequences were introduced to HEK293 cells by lentivirus,and 293T-2C9,293T-2C9 * 8 and 293T-2C9 * 27 were screened by flow cytometry and monoclonal picking.Cellular fluorescence,qRT-PCR,Western blot and the metabolic activity assays were used to identify the cells.Results MigR1-CYP2C9,MigR1-CYP2C9 * 8 and MigR1-CYP2C9 * 2293T-2C9 * 8 plasmids were constructed.All monoclonal cells 293T-2C9,293T-2C9 * 8 and 293T-2C9 * 27 cell lines expressed GFP after 30 passages.Both qRT-PCR and Western blot assay showed CYP2C9 expression in the cells.Microsomes of the three cell lines were capable of catalyzing diclofenac to 4-OH diclofenac.Conclusion 293T-2C9,293T-2C9 * 8 and293T-2C9 * 27 celllines stably expresse CYP2 C9,CYP2C9 * 8 and CYP2C9 * 27,respectively.The cell lines provide useful tools for the function investigation of CYP2C9 * 8 and CYP2C9 * 27.

Objective To construct lentiviral expression plasmids of CYP2C9,CYP2C9 * 8 and CYP2C9 * 27,and to express CYP2C9,CYP2C9 * 8 and CYP2C9 * 27 stably in HEK2C9T cells.Methods CYP2C9 coding region was obtained by reverse transcription PCR from the human liver total RNA,and then cloned into the mammalian expression vector MigR1.CYP2C9 * 8 or CYP2C9 * 27 coding sequences were obtained from MigR1-CYP2C9 by overlapping PCR and then cloned into MigR1.The coding sequences were introduced to HEK293 cells by lentivirus,and 293T-2C9,293T-2C9 * 8 and 293T-2C9 * 27 were screened by flow cytometry and monoclonal picking.Cellular fluorescence,qRT-PCR,Western blot and the metabolic activity assays were used to identify the cells.Results MigR1-CYP2C9,MigR1-CYP2C9 * 8 and MigR1-CYP2C9 * 2293T-2C9 * 8 plasmids were constructed.All monoclonal cells 293T-2C9,293T-2C9 * 8 and 293T-2C9 * 27 cell lines expressed GFP after 30 passages.Both qRT-PCR and Western blot assay showed CYP2C9 expression in the cells.Microsomes of the three cell lines were capable of catalyzing diclofenac to 4-OH diclofenac.Conclusion 293T-2C9,293T-2C9 * 8 and293T-2C9 * 27 celllines stably expresse CYP2 C9,CYP2C9 * 8 and CYP2C9 * 27,respectively.The cell lines provide useful tools for the function investigation of CYP2C9 * 8 and CYP2C9 * 27.

This study was designed to investigate the polymorphism of intron 22 (CA)n repeat within FVIII gene in Dai, Yi and Han populations of Yunnan province. PCR, DNA sequencing technique and PAGE were used in this study. The results showed that three different alleles corresponding to 24, 25 and 26 dinucleotides were found at this locus and the allele frequency ranged from 1.20% to 57.7% in Dai and from 16.43% to 63.00% in Yi populations. In Han population, five different alleles with 24, 25, 26, 27 and 28 (CA)s were found, the allele frequency ranged from 8.97% to 32.00%. Heterozygotes of intron 22 (CA) repeat within FVIII gene were not found in the three populations. In conclusion, it was obvious that the locus cannot be acted as DNA genetic marker of FVIII gene in the three populations in Yunnan.

Objective:To investigate the current status of medical record coders and their resignation tendency, and put forward suggestions for the training and team building of medical record coders.Methods:From April to May 2020, a questionnaire survey was conducted among the medical record coders in 27 DRG payment pilot hospitals in Anyang, Henan. Statistical analysis was conducted on the profile of the medical case department, basic personal information, professional quality, job satisfaction and resignation intention. Kendall coefficient was used to analyze the factors related to the resignation intention.Results:There were 98 coders in the surveyed hospitals, including 43 full-time coders and 55 part-time coders. Eighty-three valid questionnaires were recovered, and the effective recovery rate was 84.7%. Among them, 29(34.9%) had education level of junior college or below, 51(61.4%) had junior title or below, 15(18.1%) had medical information or case management as their major, 29(34.9%) coders had not obtained a coding certificate, 31(37.3%) were dissatisfied with the salary system, and 14(16.9%) had a tendency to leave. The medical record coders with lower professional titles, coding certificates and lower salary satisfaction were more likely to have the intention of resignation.Conclusions:Medical record coders in Anyang were challenged with such problems as shortage of manpower, low percentage of coding certificate holders and low salary satisfaction. Title, code certificate issuance and salary satisfaction were correlated with their resignation intention. Hospital managers should strengthen the importance of medical record coding and coders, and establish a reasonable salary, title evaluation and performance incentive system, so as to improve the attractiveness of coder position.