1.Apoptosis of K562 Cells Induced by 3,7-Dinitrodibenzobromonium Salt
Qin WANG ; Dongling YANG ; Jianzhong LU ; Shuanglai CHENG ; Zijie HOU
Chinese Journal of Cancer Biotherapy 2000;7(4):291-293
Objective: To investigate the antitumor activities and apoptosis mechanisms of 3,7-dinitrodibenzobromonium salt (cBr) on human chronic myelogenous leukemia K562 cell line in vitro. Methods: The antitumor activities of cbr were tested by Trypanblau exclusion test, MTF coloretric assay and clonal forming test, and the mechanisms were studied by morphological analysis (microscopy, fluoreseencemicroscopy, electron microscopy) and flow cytometry analysis. Resuits: cBr could inhibit of the proliferation K562 cells and kill them significantly. The effects varied with different time and doses, and IC50 of cell colony forming rate was 0. 497 mmoL/L. Apoptosis rate tested by flow cytometry was 70.34%,and apoptotic characteristics could be found by microscopy and electron microscopy. Conclusion: cBr could inhibit K562 cell proliferation greatly, and could induce K562 cells into apoptosis.
2.Clinical experience of Professor HE Sai-ping:Proved cases of gynecological diseases treated by spleen and liver
Dongxu ZHANG ; Xueqin HOU ; Zijie YIN ; Saiping HE
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(08):-
This article introduced three proved cases of gynecological disease: amenorrhea, climacteric syndrome and breast nodules, which were treated by HE Sai-ping doctor. He insisted that the gynecological diseases could be treated based on spleen and liver, of which regulating qi-blood was the therapeutic core.